Campylobacter fetus subspecies venerealis Surface Array Protein from Bovine Isolates in Brazil

Abstract

The electrophoretic patterns of 31 Campylobacter fetus subspecies venerealis capsular Surface Array Protein (SAP) isolated from bovines in reproduction from different regions of Brazil were analyzed. The persistence of the bacteria in the reproductive tract of naturally infected bovines and the dynamic of SAP expression were also evaluated. Cervical mucous and prepucial aspirates from five animals naturally infected were cultured for isolation of Campylobacter fetus and the SAPs extracted from the bacteria isolated were analyzed by SDS-PAGE. Ten different patterns of SAP expression were demonstrated by the identification of proteins with molecular mass of 97, 100, 127, and 149 kDa, respectively. The most prevalent identified protein had a molecular mass of 100 kDa (41.9%). Taking into consideration the time during which the five animals were evaluated, it was possible to conclude that one of these animals persisted with the etiological agent up to 171 days. The five naturally infected bovines analyzed presented variation on their surface protein pattern during the period of this study. C. fetus subspecies venerealis persisted in the reproductive tract of naturally infected animals. In natural condition of infection C. fetus subspecies venerealis persisted in an intermittent condition and an alteration of the protein surface was shown.

Epidemiology of Bovine Venereal Campylobacteriosis: Geographic Distribution and Recent Advances in Molecular Diagnostic Techniques

“…C. fetus has been isolated from various samples submitted for laboratory diagnosis in Canada (Brooks et al 2004); it was isolated in 2% of 1060 preputial samples screened routinely for this organism (Devenish et al 2005). BVC is considered as an important cause of reproductive failure in cattle in Brazil (Figueiredo et al 2002;Vargas et al 2002Vargas et al , 2003. In one study, Andrade et al (1986) tested 1685 bovine samples from 72 dairy herds by vaginal mucus agglutination test (VMAT) in 21 sites in Goia´s and reported a prevalence of 22.4%.…”

“…BVC is considered as an important cause of reproductive failure in cattle in Brazil (Figueiredo et al. 2002; Vargas et al. 2002, 2003).…”

The use of enzyme-linked immunosorbent assay and immunoblotting for the detection of Campylobacter fetus immunoglobulins in the cervico-vaginal mucus of female cattle

“…venerealis seems to be related to changes in SAP proteins, which migrate around 97 kDa, as suggested by several authors (Garcia et al 1995, GrogonoThomas et al 2000, Vargas et al 2002, Tu et al 2004, and that were strongly recognized in the immunoblotting assay performed. Therefore, these proteins may be an interesting alternative for testing, as they allow the identification of females with persistent C. fetus subsp.…”

“…In bovine isolates of Campylobacter fetus from bulls, cows and aborted fetus of brazilian herds, ten different patterns of SAP expression were demonstrated by identification of proteins with molecular mass of 97, 100, 127, and 149 kDa, obtained by an acid (glicin) method of extraction (Vargas et al 2002).Experiments using mouse and bovine models sugest that S-layer proteins is a dominant virulence factor enabling persistence in the genital tract (Garcia et al 1995) by inversion events resulting in expression of antigenically variant S-layers (Thompson 2002, Vargas et al 2002 .S-layer proteins conferes resistance to systemic defenses (Pei & Blaser 1990, Blaser & Pei 1993 preventing the binding of complement factor C3b to the C. fetus surface (Blaser 1988).…”

Bovine genital campylobacteriosis: main features and perspectives for diagnosis and control

“…In Brazil, BGC is among the most important causes of reproductive failure in beef and dairy farms which use natural breeding (VARGAS et al, 2002;LEAL et al, 2012;MIRANDA, 2005;OLIVEIRA et al, 2015;STYNEN et al, 2003). As shown in table 1, over the last 23 years, BGC has been reported in all regions of Brazil.…”