Calves born after open pulled straw vitrification of immature bovine oocytes

Vieira, Arnaldo Diniz; Mezzalira, Alceu; Barbieri, D. P.; Lehmkuhl, R.C; Rubin, M. I. B.; Vajta, Gábor

doi:10.1016/s0011-2240(02)00109-8

Cited by 117 publications

(90 citation statements)

References 10 publications

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“…Such as observed in our work, a low correlation between cleavage and further embryonic development has been previously stated [9,36]. Nevertheless, cleavage rates higher than 45% for oocytes vitrified both as immature and matured have been reported by other researchers [9,16]. In our work, blastocyst rates produced from vitrified, mature oocytes are lower than those presented in previous reports [5,9,10,15,16,35] and comparable to those published by Men et al [16] using immature oocytes.…”

Section: Discussionsupporting

confidence: 87%

“…In our work, blastocyst rates produced from vitrified, mature oocytes are lower than those presented in previous reports [5,9,10,15,16,35] and comparable to those published by Men et al [16] using immature oocytes. Live calves have been obtained from vitrified/ warmed immature oocytes [9].…”

Section: Discussionsupporting

confidence: 83%

“…Degeneration percentages are lower and cleavage rates are higher that those obtained by other authors [15,32,35], although development up to the morula and blastocyst stage is compromised. Such as observed in our work, a low correlation between cleavage and further embryonic development has been previously stated [9,36]. Nevertheless, cleavage rates higher than 45% for oocytes vitrified both as immature and matured have been reported by other researchers [9,16].…”

Section: Discussionsupporting

confidence: 79%

“…In our work, the COCs were submitted to a gentle vortexing for eliminating those layers of cumulus cells. This fact could be responsible for the greater sensitivity we found in vitrified/warmed oocytes and the lower developmental rates obtained after IVF, compared with the data reported by those authors [5,9]. The threedimensional COC is likely to be particularly prone to physical disruption caused by ice crystal formation [47].…”

Section: Discussionmentioning

confidence: 61%

“…Classical freezing protocols have been replaced by the most recent vitrification methods, including several modifications, such as vitrification by the open pulled straw method (OPS) [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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The nuclear stage at which oocytes are cryopreserved influences further development ability and cryopreservation affects ultrastructure of both cumulus cells and the oocyte. In this work, we analyze the effects of vitrification at different nuclear and cytoplasmic maturation stages on the oocyte ultrastructure and developmental ability. Culture in TCM199 + PVA with roscovitine 25 M during 24 h led to meiotic arrest (MA) in cumulus-oocyte complexes (COCs), while permissive in vitro maturation (IVM) was performed in TCM199, 10% FCS, FSH-LH and 17b-estradiol for 24 h. Oocytes were vitrified using the open pulled straw method (OPS) with minor modifications. Fresh and vitrified/warmed COCs were fixed as immature, after IVM, after meiotic arrest (MA) and after MA + IVM.Vitrification combined with MA followed by IVM produced the highest rates of degeneration, regardless of the vitrification time. As a consequence, lower proportions of embryos cleaved in these groups, although differences were eliminated at the five-eight cell stage. Development rates up to day 8 were similar in all experimental groups, being significantly lower than those in fresh controls. Only oocytes vitrified after IVM were able to give blastociysts. The morphological alterations observed can be responsible for compromised development. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming. #

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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Cryopreservation of Mammalian Oocytes

Anzar

2017

Animal Models and Human Reproduction

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Vitrification of pronuclear‐stage mouse embryos on solid surface (SSV) versus in cryotube: Comparison of the effect of equilibration time and different sugars in the vitrification solution

Bağış¹,

Sağırkaya²,

Mercan³

et al. 2003

Molecular Reproduction Devel

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The cryopreservation of pronuclear‐stage embryos has particular importance in transgenic technology and human assisted reproductive technology (ART). The objective of this study was to improve the efficiency of cryopreservation of pronuclear‐stage mouse embryos. Two vitrification methods (solid surface vitrification (SSV) vs. vitrification in cryotube) have been compared with special emphasis on the effect of the exposure of the embryos to the solutions for various times and the sugar content (trehalose, sucrose, or raffinose) of the vitrification solutions. Pronuclear‐stage embryos were either exposed to 1 M dimethyl sulfoxide (DMSO) + 1 M propylene‐glycol (PG) solution for 2, 5, 10, or 15 min or not exposed to this “equilibration” solution. The vitrification solutions consisted of 2.75 M DMSO and 2.75 M PG in M2 medium supplemented with 1 M trehalose (DPT), 1 M sucrose (DPS), or 1 M raffinose (DPR). In the cryotube method, groups of 15–25 embryos were transferred into a 1.8 ml cryotube containing 30 μl of DPT, DPS, or DPR. After 30 sec, the cryotubes were directly plunged into liquid nitrogen (LN2) and stored for 1 day to 1 month. Vitrified samples were warmed by immersing the cryotubes in a 40°C water bath and then immediately diluted with 300 μl of 0.3 M trehalose, sucrose, or raffinose in M2. In the SSV method, after equilibration 15–20 embryos were exposed to DPT, DPS, or DPR solutions for around 20 sec before being dropped in 2‐μl drops onto a pre‐cooled (−150 to −180°C) metal surface. Vitrified droplets were stored in cryovials in LN2. Warming was performed by transferring the vitrified droplets into 0.3 M solutions of trehalose, sucrose, or raffinose at 37°C, respectively. Results showed that both SSV and cryotube vitrification methods can result in high rates of in vitro blastocyst development (up to 58.3 and 68.5% with DPR, respectively), not statistically different from that of the controls (58.3 and 64.4%). Even without the equilibration step prior to vitrification, relatively high‐survival rates have been achieved, except for the DPS solution. In conclusion, vitrification of pronuclear‐stage mouse embryos can result in high rates of in vitro development to blastocyst, and the use of raffinose in the vitrification solution is advantageous to improve cryosurvival. Mol. Reprod. Dev. 67:186–192, 2004. © 2004 Wiley‐Liss, Inc.

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Calves born after open pulled straw vitrification of immature bovine oocytes

Cited by 117 publications

References 10 publications

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Cryopreservation of Mammalian Oocytes

Vitrification of pronuclear‐stage mouse embryos on solid surface (SSV) versus in cryotube: Comparison of the effect of equilibration time and different sugars in the vitrification solution

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