2020
DOI: 10.1007/s00204-020-02697-3
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Disruption of sensitive stages of ovary development during fetal and perinatal life can have severe and lifelong consequences for a woman's reproductive life. Exposure to endocrine disrupting chemicals may affect ovarian development, leading to subsequent reproductive disorders. Here, we investigated the effect of early life exposure to defined mixtures of humanrelevant endocrine disrupting chemicals on the rat ovary. We aimed to identify molecular events involved in pathogenesis of ovarian dysgenesis syndrome… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
3
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
5

Relationship

3
2

Authors

Journals

citations
Cited by 7 publications
(3 citation statements)
references
References 41 publications
0
3
0
Order By: Relevance
“…Correspondingly, Calb2 is expressed by androgen-producing Leydig cells of the testis (Altobelli et al 2017 ; Strauss et al 1994 ), where it has been suggested to be involved in the regulation of steroidogenesis (Xu et al 2018 ). We recently identified Calb2 as a putative biomarker for female reproductive toxicity by performing a proteomics screen on rat ovaries exposed during development to a mixture of environmental chemicals (Johansson et al 2020 ). Subsequently, we have observed dysregulated Calb2 expression in fetal rat testis exposed to flusilazole (Draskau et al 2021 ).…”
Section: Discussionmentioning
confidence: 99%
“…Correspondingly, Calb2 is expressed by androgen-producing Leydig cells of the testis (Altobelli et al 2017 ; Strauss et al 1994 ), where it has been suggested to be involved in the regulation of steroidogenesis (Xu et al 2018 ). We recently identified Calb2 as a putative biomarker for female reproductive toxicity by performing a proteomics screen on rat ovaries exposed during development to a mixture of environmental chemicals (Johansson et al 2020 ). Subsequently, we have observed dysregulated Calb2 expression in fetal rat testis exposed to flusilazole (Draskau et al 2021 ).…”
Section: Discussionmentioning
confidence: 99%
“…The cell debris containing cell walls was washed 5 times in 1 M NaCl to remove cytoplasmic contamination, resuspended in buffer (500 mM Tris-HCl buffer [pH 7.5], 2% [w/v] SDS, 0.3 M β-mercaptoethanol, and 1 mM EDTA), boiled 3 times at 100 ºC for 10 min and freeze-dried. The pellets were digested with trypsin according to the PRIME-XS protocol [ 38 ]. Mass spectrometry was performed using a Q Exactive Plus (Thermo Fisher Scientific) and tryptic peptides were identified using the MASCOT search engine (Matrix Science) [ 39 ].…”
Section: Methodsmentioning
confidence: 99%
“…The possibility of including more female KEs and AOs and thereby enhancing some of the in vivo Test Guidelines could be a way forward in the shorter term. So too, the discovery of robust biomarkers for female reproductive disorders, for instance the recently suggested CALB2 (Johansson et al 2020 ), could prove very valuable additions to testing strategies as well as for clinical purposes.…”
Section: Perspectivesmentioning
confidence: 99%