Calorie Restriction Attenuates Terminal Differentiation of Immune Cells

White, Matthew; Beaver, Charlotte; Goodier, Martin R.; Bottomley, Christian; Nielsen, Carolyn M.; Wolf, Asia-Sophia; Boldrin, Luisa; Whitmore, C.; Morgan, Jennifer E.; Pearce, Daniel J.; Riley, Eleanor M.

doi:10.3389/fimmu.2016.00667

Cited by 26 publications

(23 citation statements)

References 69 publications

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“…Previous work has shown that immune responses can be strongly affected by the amount and/or balance of nutrients in the diet e.g. (Fernandes et al, 1976; Ingram et al, 1995; Kristan, 2008; Le Couteur et al, 2015; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; Ritz and Gardner, 2006; Wallace et al, 1999; White et al, 2017). However, most of these studies covered only a relatively small region of nutrient space (Fernandes et al, 1976; Ingram et al, 1995; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; Ritz and Gardner, 2006; White et al, 2017) and/or only tested innate responses (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…(Fernandes et al, 1976; Ingram et al, 1995; Kristan, 2008; Le Couteur et al, 2015; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; Ritz and Gardner, 2006; Wallace et al, 1999; White et al, 2017). However, most of these studies covered only a relatively small region of nutrient space (Fernandes et al, 1976; Ingram et al, 1995; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; Ritz and Gardner, 2006; White et al, 2017) and/or only tested innate responses (e.g. (Fernandes et al, 1976; Ingram et al, 1995; Le Couteur et al, 2015; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; White et al, 2017) or the response to an artificial pathogen or immune stimulant (Cotter et al, 2011b).…”

Section: Discussionmentioning

confidence: 99%

“…However, most of these studies covered only a relatively small region of nutrient space (Fernandes et al, 1976; Ingram et al, 1995; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; Ritz and Gardner, 2006; White et al, 2017) and/or only tested innate responses (e.g. (Fernandes et al, 1976; Ingram et al, 1995; Le Couteur et al, 2015; Lee et al, 2006; Nayak et al, 2009; Povey et al, 2009; White et al, 2017) or the response to an artificial pathogen or immune stimulant (Cotter et al, 2011b). Here we addressed this gap by looking at both gene expression, functional immune responses and survival after both dead and live pathogen challenges over a broad region of nutrient space.…”

Section: Discussionmentioning

confidence: 99%

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Diet modulates the relationship between immune gene expression and functional immune responses

Cotter

Reavey

Tummala

et al. 2019

Insect Biochemistry and Molecular Biology

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Nutrition is vital to health and the availability of resources has long been acknowledged as a key factor in the ability to fight off parasites, as investing in the immune system is costly. Resources have typically been considered as something of a “black box”, with the quantity of available food being used as a proxy for resource limitation. However, food is a complex mixture of macro- and micronutrients, the precise balance of which determines an animal's fitness. Here we use a state-space modelling approach, the Geometric Framework for Nutrition (GFN), to assess for the first time, how the balance and amount of nutrients affects an animal's ability to mount an immune response to a pathogenic infection. Spodoptera littoralis caterpillars were assigned to one of 20 diets that varied in the ratio of macronutrients (protein and carbohydrate) and their calorie content to cover a large region of nutrient space. Caterpillars were then handled or injected with either live or dead Xenorhabdus nematophila bacterial cells. The expression of nine genes (5 immune, 4 non-immune) was measured 20 h post immune challenge. For two of the immune genes (PPO and Lysozyme) we also measured the relevant functional immune response in the hemolymph. Gene expression and functional immune responses were then mapped against nutritional intake. The expression of all immune genes was up-regulated by injection with dead bacteria, but only those in the IMD pathway (Moricin and Relish) were substantially up-regulated by both dead and live bacterial challenge. Functional immune responses increased with the protein content of the diet but the expression of immune genes was much less predictable. Our results indicate that diet does play an important role in the ability of an animal to mount an adequate immune response, with the availability of protein being the most important predictor of the functional (physiological) immune response. Importantly, however, immune gene expression responds quite differently to functional immunity and we would caution against using gene expression as a proxy for immune investment, as it is unlikely to be reliable indicator of the immune response, except under specific dietary conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diet modulates the relationship between immune gene expression and functional immune responses

Cotter

Reavey

Tummala

et al. 2019

Insect Biochemistry and Molecular Biology

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“…Moreover, metabolic status could affect the functions of immune cells. Caloric restriction has exhibited systemic antiinflammatory effects, along with attenuated terminal differentiation of immune cells (111). Given the energy sensing properties of AMPK, the AMPK-ULK1-TBK1 axis may also function in immune cells to mediate anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Adipocyte Metabolic and Immune Functions Through TBK1

Zhao

Saltiel

2020

Front. Immunol.

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Adipocytes and adipose tissue play critical roles in the regulation of metabolic homeostasis. In obesity and obesity-associated metabolic diseases, immune cells infiltrate into adipose tissues. Interaction between adipocytes and immune cells reshapes both metabolic and immune properties of adipose tissue and dramatically changes metabolic set points. Both the expression and activity of the non-canonical IKK family member TBK1 are induced in adipose tissues during diet-induced obesity. TBK1 plays important roles in the regulation of both metabolism and inflammation in adipose tissue and thus affects glucose and energy metabolism. Here we review the regulation and functions of TBK1 and the molecular mechanisms by which TBK1 regulates both metabolism and inflammation in adipose tissue. Finally, we discuss the potential of a TBK1/IKKe inhibitor as a new therapy for metabolic diseases.

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“…The thymus is one of the major lymphoid organs in the immune system, and several studies have reported that calorie restriction (CR) potentiates thymic function [ 2 , 28 , 60 ]. RNA were isolated from thymus tissue of ad libitum, chronic calorie restriction, and intermittent calorie restriction diet MMTV-TGF- α mice from 10 weeks of age to 17 weeks of age or 18 weeks of age.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Thymus in Short-Term Calorie-Restricted Mice Using RNA-seq

Ulu

Doğan

et al. 2018

International Journal of Genomics

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Calorie restriction (CR), which is a factor that expands lifespan and an important player in immune response, is an effective protective method against cancer development. Thymus, which plays a critical role in the development of the immune system, reacts to nutrition deficiency quickly. RNA-seq-based transcriptome sequencing was performed to thymus tissues of MMTV-TGF-α mice subjected to ad libitum (AL), chronic calorie restriction (CCR), and intermittent calorie restriction (ICR) diets in this study. Three cDNA libraries were sequenced using Illumina HiSeq™ 4000 to produce 100 base pair-end reads. On average, 105 million clean reads were mapped and in total 6091 significantly differentially expressed genes (DEGs) were identified (p < 0.05). These DEGs were clustered into Gene Ontology (GO) categories. The expression pattern revealed by RNA-seq was validated by quantitative real-time PCR (qPCR) analysis of four important genes, which are leptin, ghrelin, Igf1, and adinopectin. RNA-seq data has been deposited in NCBI Gene Expression Omnibus (GEO) database (GSE95371). We report the use of RNA sequencing to find DEGs that are affected by different feeding regimes in the thymus.

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Calorie Restriction Attenuates Terminal Differentiation of Immune Cells

Cited by 26 publications

References 69 publications

Diet modulates the relationship between immune gene expression and functional immune responses

Diet modulates the relationship between immune gene expression and functional immune responses

Interaction of Adipocyte Metabolic and Immune Functions Through TBK1

Transcriptome Analysis of the Thymus in Short-Term Calorie-Restricted Mice Using RNA-seq

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