Callus induction and plant regeneration via anther culture in mutant tomato (Lycopersicon esculentum Mill.) lines with anther abnormalities

Abstract

Anthers from two tomato mutants, solanifolia (sf) and trifoliate (tf), characterized by anther abnormalities, 4 male sterile tomato mutants (ms 15 26 , ms 30, ms 32, and ms 33), and their isogenic fertile lines were cultured in vitro, using three modifications of MS media. The frequency of callus induction in the mutant anthers was significantly higher than in the wild type ones in all genotypes, a result independent of the medium. Pseudofruits surrounded by well-developed calli appeared from the anthers of San Marzano ms 15, Van's Early ms 26, San Marzano ms 30, and VF 11 Pearson ms 33. The higher callus induction in the mutant anthers correlated with higher frequency of organogenesis.The fact that, in addition to the ms-mutants, solanifolia and trifoliate also affected callus frequency, suggested that in tomato, process/es leading to, or resulting from anther abnormalities might be favorable for enhancing anther callus induction and subsequent regeneration.Abbreviations: GA 3 -gibberellic acid; IAA-indole-3-acetic acid; IBA-indole-3-butyric acid; IL/NIL-isogenic/near isogenic line; ms-male sterility; MS-Murashige and Skoog medium; NAA-1-naphthaleneacetic acid; sf-solanifolia; tf-trifoliate; 2 iP-6-(γ, γ-dimethylallylamino)purine

Research on in vitro haploidization of heterozygotic breeding lines of tomato (Solanum lycopersicum L.)

“…In the presented studies, callus was obtained in 9 breeding lines, which is a half of the 18 lines used in our experiments. Shtereva and Atanassova (2001) were able to induce embryogenesis in all wild mutants of tomato, but the efficiency of this process was different depending on the genotype. Similarly to our studies, 253.94 callus structures per 100 anthers were obtained in the most embryogenic line no.…”

Aspectos básicos y aplicados de la inducción de embriogénesis en microsporas de pimiento y colza

“…La especie en la que más se ha estudiado esta ruta ha sido el tomate (Solanum lycopersicum). A pesar de conseguir la regeneración de plantas completas, la eficiencia fue muy baja y los regenerantes presentaron un elevado porcentaje de mixoploidía (Shtereva y Atanassova, 2001;Zagorska et al, 2004;Seguí-Simarro y Nuez, 2007;Corral-Martínez et al, 2011). Pese a la importancia de esta especie, los resultados obtenidos hasta ahora son insuficientes para ser trasladados a las empresas productoras de semilla híbrida de tomate.…”