Callogenesis optimization and cell suspension culture establishment of Dracocephalum polychaetum Bornm. and Dracocephalum kotschyi Boiss.: An in vitro approach for secondary metabolite production

Taghizadeh, Marzieh; Nasibi, Fatemeh; Kalantari, Khosrow Manouchehri; Benakashani, Fatemeh

doi:10.1016/j.sajb.2020.04.015

Cited by 10 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These ndings suggest that light modulates I. amara traits in vitro by physiological changes. Similar observations with 16-h light/8-h dark photoperiod for improving callus induction and callus growth rate were previously recorded for D. polychaetum and D. kotschyi (Taghizadeh et al 2020).…”

Section: Optimum Callus Induction Mediumsupporting

confidence: 88%

“…MDA is a product of lipid peroxidation that indicates free-radical accumulations and oxidative stress. Improved membrane permeability and disrupted membrane integrity were possible reasons for the induction of oxidative stress (Taghizadeh et al 2020). Further, the content of MDA increased remarkably in the cells that were elicited with 100 and 200 ppm chitosan compared with the control (Fig.…”

Section: Lipid Peroxidationmentioning

confidence: 87%

“…Chitosan affects plant cells via inducing oxidative stress and increasing activity, lifetime, and concentrations of free radicals. The possible causing factor for the induction of oxidative stress disrupted membrane integrity, improved lipid peroxidation and cell toxicity, and then caused cell death (Taghizadeh et al 2020). In other words, these compounds have possibly a vital role as free radical scavenging in I. amara cells subjected to chitosan elicitation.…”

Section: Trait Correlationsmentioning

confidence: 99%

“…Regarding diverse responses to hormones, it can be concluded that PGRs are critical factors that are responsible for I. amara suspension establishment in cell culture. From previous reports, the advisable concentration of these hormones is dependent on explant type, genotype, and explant origin(Mathur and Shekhawat 2013;Taghizadeh et al 2020).…”

mentioning

confidence: 92%

See 3 more Smart Citations

Enhancement Production of Phenolic Compounds in The Cell Suspension Culture of Iberis Amara L.: The Effect of Chitosan Elicitation

Taghizadeh

Nekonan²,

Setorki

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Iberis amara L. medicinal herb is well-known for having pharmacological values although its use has been challenged by the low levels of secondary metabolites. To address this issue, this study focused on evaluating the effect of explant, photoperiod, and plant growth regulators (PGRs) to find the optimum medium for inducing callus and establishing cell suspension in I. amara, followed by investigating the chitosan effect on some secondary metabolites. From our observations, the optimum condition for induced callus was achieved from the leaf explants in Murashige and Skoog (MS) media completed with 3 mg L− 1 6-benzylaminopurine(BAP) and 1 mgL− 1 1-naphthalene acetic acid(NAA) under 16-h light/8-h dark photoperiod. The MS enhanced with 3 mgL− 1BAP, 1 mg L− 1NAA, and 2% (w/v) sucrose appeared to be optimum conditions for suspension establishment. Thus, the cells were exposed to different concentrations of chitosan (200, 100, 50, and 0 mg L− 1) in their exponential growth stage from day 8 to 12 and day 12 to 16 following sub-cultures (T1) and sub-cultures (T2), respectively. The results showed that the 50 mgL− 1 chitosan significantly improved the total phenol, flavonoid, flavonol, and anthocyanin content in the I. amara in a dose-dependent manner. The highest malondialdehyde (MDA) amount, as a result of lipid peroxidation, was observed under the 200 ppm chitosan elicitation. Overall, these novel findings demonstrated the possibility of applying the cell suspension of I. amara treated with chitosan as a helpful approach for improving synthesizing phenolic compounds under controlled and sterile conditions without genetic modifications in medicinal herbs.

show abstract

Section: Optimum Callus Induction Mediumsupporting

confidence: 88%

Section: Lipid Peroxidationmentioning

confidence: 87%

Section: Trait Correlationsmentioning

confidence: 99%

mentioning

confidence: 92%

See 2 more Smart Citations

Enhancement Production of Phenolic Compounds in The Cell Suspension Culture of Iberis Amara L.: The Effect of Chitosan Elicitation

Taghizadeh

Nekonan²,

Setorki

2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Alternatively, the biotechnological production of SMs through in vitro plant cell, tissue, and organ culture is an efficient and faster method that guarantees stable and controlled conditions. These in vitro techniques have been widely used for the production of several SMs, notably taxol [ 31 , 32 , 33 ], podophyllotoxin [ 34 , 35 , 36 ], withanolides [ 37 ], centellosides [ 38 , 39 ], and rosmarinic acid (RA) [ 40 , 41 , 42 , 43 ], as well as for mass propagation [ 44 ], in vitro cloning [ 45 , 46 , 47 ], and polyploidization [ 48 , 49 ]. Many RA-producing biotechnological platforms have been established, based on shoots [ 50 , 51 , 52 ], callus [ 53 ], cells [ 41 , 42 , 54 ], and hairy root cultures [ 55 , 56 , 57 , 58 ] of numerous species of the Boraginaceae, Anthocerotaceae, and Lamiaceae.…”

Section: Introductionmentioning

confidence: 99%

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

et al. 2020

View full text Add to dashboard Cite

Modern lifestyle factors, such as physical inactivity, obesity, smoking, and exposure to environmental pollution, induce excessive generation of free radicals and reactive oxygen species (ROS) in the body. These by-products of oxygen metabolism play a key role in the development of various human diseases such as cancer, diabetes, heart failure, brain damage, muscle problems, premature aging, eye injuries, and a weakened immune system. Synthetic and natural antioxidants, which act as free radical scavengers, are widely used in the food and beverage industries. The toxicity and carcinogenic effects of some synthetic antioxidants have generated interest in natural alternatives, especially plant-derived polyphenols (e.g., phenolic acids, flavonoids, stilbenes, tannins, coumarins, lignins, lignans, quinines, curcuminoids, chalcones, and essential oil terpenoids). This review focuses on the well-known phenolic antioxidant rosmarinic acid (RA), an ester of caffeic acid and (R)-(+)-3-(3,4-dihydroxyphenyl) lactic acid, describing its wide distribution in thirty-nine plant families and the potential productivity of plant sources. A botanical and phytochemical description is provided of a new rich source of RA, Satureja khuzistanica Jamzad (Lamiaceae). Recently reported approaches to the biotechnological production of RA are summarized, highlighting the establishment of cell suspension cultures of S. khuzistanica as an RA chemical biofactory.

show abstract

In Vitro Production of Phenolic Compound

Giri

Singh

Bhatt

2022

Nutraceuticals Production From Plant Cell Factory

View full text Add to dashboard Cite

Callogenesis optimization and cell suspension culture establishment of Dracocephalum polychaetum Bornm. and Dracocephalum kotschyi Boiss.: An in vitro approach for secondary metabolite production

Cited by 10 publications

References 35 publications

Enhancement Production of Phenolic Compounds in The Cell Suspension Culture of Iberis Amara L.: The Effect of Chitosan Elicitation

Enhancement Production of Phenolic Compounds in The Cell Suspension Culture of Iberis Amara L.: The Effect of Chitosan Elicitation

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

In Vitro Production of Phenolic Compound

Contact Info

Product

Resources

About