Callistemon citrinus bioactive metabolites as new inhibitors of methicillin-resistant Staphylococcus aureus biofilm formation

Shehabeldine, Amr M.; Ashour, Rehab M.; Okba, Mona M.; Saber, Fatema R.

doi:10.1016/j.jep.2020.112669

Cited by 52 publications

(36 citation statements)

References 47 publications

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“…The antimicrobial and antibiofilm activity of Pimenta spp. E.Os presented herein, is in agreement with recent studies reporting the potential of myrtaceous plants as efficient inhibitors of bacterial quorum sensing and to possess pronounced antimicrobial and antibiofilm activities [ 18 , 22 ]. A study performed by Vasavi et al [ 23 ] revealed a strong antiquorum sensing activity for the ethyl acetate fraction of ethanolic extract of P. dioica, this fraction could inhibit AHL-mediated violacein pigment production in Chromobacterium violaceum, ATCC12472, at dose-dependent manner, the authors related this effect to polyphenolic compounds like the E.O components of the tested extract.…”

Section: Resultssupporting

confidence: 92%

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

et al. 2020

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Bacterial biofilm contributes to antibiotic resistance. Developing antibiofilm agents, more favored from natural origin, is a potential method for treatment of highly virulent multidrug resistant (MDR) bacterial strains; The potential of Pimenta dioica and Pimenta racemosa essential oils (E.Os) antibacterial and antibiofilm activities in relation to their chemical composition, in addition to their ability to treat Acinetobacter baumannii wound infection in mice model were investigated; P. dioica leaf E.O at 0.05 µg·mL−1 efficiently inhibited and eradicated biofilm formed by A. baumannii by 85% and 34%, respectively. Both P. diocia and P. racemosa leaf E.Os showed a bactericidal action against A. baumanii within 6h at 2.08 µg·mL−1. In addition, a significant reduction of A. baumannii microbial load in mice wound infection model was found. Furthermore, gas chromatography mass spectrometry analysis revealed qualitative and quantitative differences among P. racemosa and P. dioica leaf and berry E.Os. Monoterpene hydrocarbons, oxygenated monoterpenes, and phenolics were the major detected classes. β-Myrcene, limonene, 1,8-cineole, and eugenol were the most abundant volatiles. While, sesquiterpenes were found as minor components in Pimenta berries E.O; Our finding suggests the potential antimicrobial activity of Pimenta leaf E.O against MDR A. baumannii wound infections and their underlying mechanism and to be further tested clinically as treatment for MDR A. baumannii infections.

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Section: Resultssupporting

confidence: 92%

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

et al. 2020

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“…As a part of our continuing effort to examine plants of Myrtaceae family for their bioactive principles 14,15 , the present study was designed to further investigate the chemical constituents of Feijoa leaves. Additionally, different in vitro assays (antioxidant and enzyme inhibition) were conducted to provide some additional insights and perspectives into the possible health benefits of F. sellowiana in management of different human diseases and possible mechanistic insights by performing the same assays for the major encompassed chemical constituents.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical profile, enzyme inhibition activity and molecular docking analysis ofFeijoa sellowianaO. Berg

Saber

Ashour

El-Halawany

et al. 2021

Journal of Enzyme Inhibition and Medicinal Chemistry

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Feijoa sellowiana leaves and fruits have been investigated as a source of diverse bioactive metabolites. Extract and eight metabolites isolated from F. sellowiana leaves were evaluated for their enzymatic inhibitory activity against a-glucosidase, amylase, tyrosinase, acetylcholinestrerase and butyrylcholinesterase both in vitro and in silico. Feijoa leaves' extract showed strong antioxidant activity and variable levels of inhibitions against target enzymes with a strong anti-tyrosinase activity (115.85 mg Kojic acid equivalent/g). Additionally, a-tocopherol emerged as a potent inhibitor of AChE and BChE (5.40 & 10.38 mmol galantamine equivalent/g, respectively). Which was further investigated through molecular docking and found to develop key enzymatic interactions in AChE and BChE active sites. Also, primetin showed good anti BChE (11.70 mmol galantamine equivalent/g) and anti-tyrosinase inhibition (90.06 mmol Kojic acid equivalent/g) which was also investigated by molecular docking studies. HIGHLIGHTS Isolation of eight bioactive constituents from Feijoa sellowiana leaves. In vitro assays using different enzymatic drug targets were investigated. In silico study was performed to define compound interactions with target proteins. Feijoa leaf is an excellent source of anti-AChE and antityrosinase bioactives.

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“…The covers were carefully cleaned using 0.01 M PBS to eliminate the non-adhered Candida cells and colored with 500 μL of SYTO 9 (gray fluorescent with viable cells) and propidium iodide (red green fluorescent with dead cells) mixed dye solution after the incubation period. The Live/Dead ® BacLightTM Bacterial Viability Kits were used for 15 min in dark and ambient conditions [22]. Subsequently, a ZEISS LSM 710 confocal laser scanning microscope (CLSM; Carl Zeiss, Jena, Germany) was used for imaging [23].…”

Section: Visualization Of Biofilm Inhibition By Confocal Laser Scannimentioning

confidence: 99%

Enhancing the Antifungal Activity of Griseofulvin by Incorporation a Green Biopolymer-Based Nanocomposite

et al. 2021

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Fungal biofilms have caused several medical problems, resulting in significant morbidity and mortality as well as poor response to antifungal drugs. The current study was designed to evaluate the enhancement of antifungal and anti-biofilm activity of Griseofulvin-loaded green nanocomposite-based biopolymers (Ge-Nco) of glycogen and gelatin against different strains of pathogenic Candida species. The prepared Ge-Nco was characterized using Fourier-transform infrared (FT-IR), X-ray diffraction pattern (XRD), scanning electron microscopy-energy dispersive X-ray (SEM-EDX) and transmission electron microscope (TEM). In addition, the morphology of the mature biofilm and the inhibition of biofilm was monitored and visualized using confocal laser scanning microscopy (CLSM). The minimal inhibitory concentrations (MIC) and (IC50) of Griseofulvin alone and the prepared Ge-Nco against three different strains of Candida sp. were determined according to Clinical and Laboratory Standards Institute (CLSI) method. The effects of Griseofulvin alone and Ge-Nco on the tested Candida sp. biofilm formation were determined by the crystal-violet staining protocol. The biofilm inhibition potential of Ge-Nco against the tested Candida sp. was detected and depicted under CLSM (2.5 D view). The findings depicted that Ge-Nco was prepared in nanometer size (10–23 nm). The observed minimum inhibitory concentration (MIC) of Griseofulvin alone and Ge-Nco against three different Candida sp. were found to be in range 49.9–99.8 μg/mL and 6.24–12.48 μg/mL, respectively. These results provide evidence for implementing efficient antivirulence approaches against three different Candida sp. that would be less likely to foster the emergence of resistance.

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Callistemon citrinus bioactive metabolites as new inhibitors of methicillin-resistant Staphylococcus aureus biofilm formation

Cited by 52 publications

References 47 publications

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

Phytochemical profile, enzyme inhibition activity and molecular docking analysis ofFeijoa sellowianaO. Berg

Enhancing the Antifungal Activity of Griseofulvin by Incorporation a Green Biopolymer-Based Nanocomposite

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