Calli induction in leaf explants of coffee elite genotypes

Rezende, Juliana Costa de; Carvalho, C. H. S.; Pasqual, Moacir; Santos, Ana Carolina Ramia; Carvalho, Stephan Malfitano

doi:10.1590/s0103-84782011000300004

Cited by 6 publications

(4 citation statements)

References 13 publications

(13 reference statements)

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“…Catuaí from 250 mg of fresh callus weight (5.6 times) grown in 125 mL Erlenmeyer flasks with 25 mL of liquid medium, for four weeks in darkness. Likewise, de Rezende et al [ 25 ] observed that the calli of Clone 3 [Siriema ( C. racemose × C. arabica )] grown in MS salts at 50%, 1.1 mg L −1 2,4-D, 1.0 mg L −1 indole butyric acid (IBA), and 2.0 mg L −1 2-isopentenyladenine (2iP) for 63 days (with a subculture every 21 days), increased their fresh weight by 7 times, while those of Clone 28 (cross of Red Catuaí IAC 44 × Timor Hybrid CIFC 2750) increased their biomass by 9.8 times, under the same growing conditions.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Mass Propagation of Coffee Plants (Coffea arabica L. var. Colombia) through Indirect Somatic Embryogenesis

Avila-Victor

Chaparro

Arjona-Suárez

et al. 2023

Plants

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Coffea arabica is one of the two most consumed coffee species in the world. Micropropagation through somatic embryogenesis has allowed the large-scale propagation of different coffee varieties. However, the regeneration of plants using this technique depends on the genotype. This study aimed to develop a protocol for the regeneration of C. arabica L. var. Colombia by somatic embryogenesis for its mass propagation. Foliar explants were cultured on Murashige and Skoog (MS) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), and phytagel for inducing somatic embryogenesis. In total, 90% of the explants formed embryogenic calli with a culture medium containing 2 mg L−1 of 2,4-D, 0.2 mg L−1 BAP, and 2.3 g L−1 phytagel. The highest number of embryos per gram of callus (118.74) was obtained in a culture medium containing 0.5 mg L−1 2,4-D, 1.1 mg L−1 BAP, and 5.0 g L−1 phytagel. In total, 51% of the globular embryos reached the cotyledonary stage when they were cultured on the growth medium. This medium contained 0.25 mg L−1 BAP, 0.25 mg L−1 indoleacetic acid (IAA), and 5.0 g L−1 of phytagel. The mixture of vermiculite:perlite (3:1) allowed 21% of embryos to become plants.

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Section: Discussionmentioning

confidence: 99%

In Vitro Mass Propagation of Coffee Plants (Coffea arabica L. var. Colombia) through Indirect Somatic Embryogenesis

Avila-Victor

Chaparro

Arjona-Suárez

et al. 2023

Plants

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“…However, the embryogenic sectors multiplication as well as regeneration and maturation of somatic embryos to plantlets conversion was only possible by adapting protocols (Zamarripa et al 1991;Van Boxtel and Berthouly 1996;Teixeira et al 2004;Maciel et al 2016). It is well known that the genotype can influence tissue culture, in the case of somatic embryogenesis the formation of embryogenic sectors can be altered depending on the cultivar used (Quiroz-Figueroa Teixeira et al 2004;Gatica et al 2007;Rezende et al 2011;Sané et al 2012;Beena et al 2014;Verma et al 2016). Too many different protocols exist and cultivars display distinct outcomes to the same protocol in coffee (Campos et al 2017).…”

Section: Discussionmentioning

confidence: 99%

In silico and in vivo analysis of ABI3 and VAL2 genes during somatic embryogenesis of Coffea arabica: competence acquisition and developmental marker genes

Freitas

Barreto

Torres

et al. 2019

Plant Cell Tiss Organ Cult

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The employment of biotechnology-based approaches such as somatic embryogenesis has been applied to several plants including Coffea sp. Despite the economic importance of this genus, few information about the role of key regulatory genes in somatic embryogenesis in coffee is available. This work provides information about ABI3 and VAL2 genes performance by RT-qPCR during indirect somatic embryogenesis of Coffea arabica. To achieve this, bioinformatics analysis was performed to identify the genes of the B3 superfamily in the coffee genome. The cell suspensions lines presented similar histological and regeneration patterns, yielding of up to 6.6 embryos per 1 mg of embryogenic aggregates at 7 months. We have identified possible orthologs for VAL2 (Cc06g00410) and ABI3 (Cc01g17380) as well as the other members belonging to superfamily B3. The CaABI3 expression was higher in dedifferentiated competent cells for somatic embryogenesis as compared with non-embryogenic calli. Whereas the expression of VAL2 gene is more active in cotyledonary embryos and plantlets, showing its clear performance in the embryogenesis late stages. The present study suggests that CaABI3 gene could be potentially used as a biomarker for embryogenic process improvement. The good plantlets development obtained from the protocol used may be a reflection of the high expression of CaVAL2 in cotyledonary embryos and plantlets. Key message The activity of CaABI3 is correlated to embryogenic potential with highly expressed in embryogenic masses and expression of the VAL2 gene is increased at the end of the embryogenic process.

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“…Genotypic differences play a significant role in callus formation, as exhibited in coffee (REZENDE et al, 2011). These differences correspond to the variation in endogenous levels of growth regulators of explants associated to culture conditions that influence the formation and development of the callus (SMITH, 2013).…”

Section: Calli Induction and Growth Curve From Nodal Explantsmentioning

confidence: 99%

Induction and growth curve of calli from leaf and nodal explants of genipap

Oliveira¹,

Machado²,

Oliveira³

et al. 2018

Biosci. J.

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ASBTRACT: The aim of this study was to determine the effect of the auxin 2,4-D (2,4-dichlorophenoxyacetic) in calli formation from leaf and nodal segments of genipap and to characterize its growth curve. Explants obtained from shoots previously established from in vitro seedlings were used for calli induction. The experimental design was completely randomized in a 3x5x2 factorial with three accessions (NB, SA, SAL), five concentrations of 2,4-D (0.0; 2.0; 4.0, 6.0 or 8.0 mg L -1 ) and two times of measurement for calli fresh weight (30 and 60 days). There was callus formation in all treatments tested. It was observed that the best response for callus induction from leaf segments was with 2.0 mg L -1 of 2,4-D. For the nodal segment, the response among the accessions was different due to 2,4-D concentrations. The growth curve was plotted according to the fresh weight of callus obtained at intervals of 10 days up to 60 days. Through the established growth curve, the nodal-derived calli from accession SA should be transferred to a new medium, after 40 days of culture.

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Calli induction in leaf explants of coffee elite genotypes

Cited by 6 publications

References 13 publications

In Vitro Mass Propagation of Coffee Plants (Coffea arabica L. var. Colombia) through Indirect Somatic Embryogenesis

In Vitro Mass Propagation of Coffee Plants (Coffea arabica L. var. Colombia) through Indirect Somatic Embryogenesis

In silico and in vivo analysis of ABI3 and VAL2 genes during somatic embryogenesis of Coffea arabica: competence acquisition and developmental marker genes

Induction and growth curve of calli from leaf and nodal explants of genipap

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