Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),M(2+)-АТРase in myometrial cells

Veklich, Т. O.; Skrabak, O. A.; Nikonishyna, Yu. V.; Rodik, Roman V.; Kаlchеnkо, Vitaly I.; Костерін, С. О.

doi:10.15407/ubj90.05.034

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References 33 publications

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“…It should be noted that the PM of uterine myocytes was found to also contain Са 2+ -АТРase, the properties of which differed from Са 2+ ,Mg 2+ -АТРase, since its activity was manifested in the presence of millimolar concentrations of Са 2+ and АТР in the incubation medium against the background of Mg ions [29,30]. Са 2+ -АТРase was of low affinity to the activating cation, the constant of activation with Ca ions (K Са ) was 1 mM [30].…”

Section: Palladin Institute Of Biochemistry National Academy Of Scien...mentioning

confidence: 99%

Thiacalix[4]arene С-1087 is the selective inhibitor of the calcium pump of smooth muscle cells plasma membrane

Veklich,

Rodik,

Tsymbalyuk

et al. 2023

Ukr.Biochem.J.

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The enzymatic and kinetic analyses were used to demonstrate that 5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)methylamino-25,27-dihexyloxy-26,28-dihydroxythiacalix[4]arene С-1087 effectively inhibited the Са2+,Mg2+-АТРase activity of the rat myometrium cells plasma membrane (І0.5 = 9.4 ± 0.6 µM) with no effect on the relative activity of other membrane ATPases. With the use of confocal microscopy and Ca2+-sensitive fluorescent probe fluo-4, it was shown that the application of thiacalix[4]arene С-1087 to the immobilized uterus myocytes increased the cytosolic concentration of Ca2+. Tenzometric studies of rat uterus smooth muscles with the subsequent mechanokinetic analysis revealed that thiacalix[4]arene С-1087 considerably decreased the maximal velocity of the relaxation of both spontaneous contractile response and contraction induced by hyperpotassium solution. Keywords: contraction-relaxation mechanokinetics, cytosolic Ca(2+), myometrium, plasma membrane Са(2+)‚Mg(2+)-АТРase, smooth muscle cell, thiacalix[4]arene

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Section: Palladin Institute Of Biochemistry National Academy Of Scien...mentioning

confidence: 99%

Thiacalix[4]arene С-1087 is the selective inhibitor of the calcium pump of smooth muscle cells plasma membrane

Veklich,

Rodik,

Tsymbalyuk

et al. 2023

Ukr.Biochem.J.

Self Cite

View full text Add to dashboard Cite

show abstract

Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),M(2+)-АТРase in myometrial cells

Cited by 1 publication

References 33 publications

Thiacalix[4]arene С-1087 is the selective inhibitor of the calcium pump of smooth muscle cells plasma membrane

Thiacalix[4]arene С-1087 is the selective inhibitor of the calcium pump of smooth muscle cells plasma membrane

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