Calcium phosphate formation in aqueous suspensions of multilamellar liposomes

Eanes, E. D.; Hailer, A. W.; Costa, Jonathan L.

doi:10.1007/bf02405354

Cited by 44 publications

(25 citation statements)

References 32 publications

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“…There have been previous attempts to simulate MV mineralization using multilamellar liposomal systems (Eanes et al 1984;Eanes andHailer 1985, 1987;Eanes 1989). In addition, liposomes containing various proportions of anionic and neutral phospholipids and cholesterol were used to study the ability of annexin V to facilitate Ca 2+ uptake (Kirsch et al 1997).…”

Section: New Perspectives For Applications Of Proteoliposomesmentioning

confidence: 99%

Proteoliposomes in nanobiotechnology

et al. 2012

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Proteoliposomes are systems that mimic lipid membranes (liposomes) to which a protein has been incorporated or inserted. During the last decade, these systems have gained prominence as tools for biophysical studies on lipid-protein interactions as well as for their biotechnological applications. Proteoliposomes have a major advantage when compared with natural membrane systems, since they can be obtained with a smaller number of lipidic (and protein) components, facilitating the design and interpretation of certain experiments. However, they have the disadvantage of requiring methodological standardization for incorporation of each specific protein, and the need to verify that the reconstitution procedure has yielded the correct orientation of the protein in the proteoliposome system with recovery of its functional activity. In this review, we chose two proteins under study in our laboratory to exemplify the steps necessary for the standardization of the reconstitution of membrane proteins in liposome systems: (1) alkaline phosphatase, a protein with a glycosylphosphatidylinositol anchor, and (2) Na,K-ATPase, an integral membrane protein. In these examples, we focus on the production of the specific proteoliposomes, as well as on their biochemical and biophysical characterization, with emphasis on studies of lipid-protein interactions. We conclude the chapter by highlighting current prospects of this technology for biotechnological applications, including the construction of nanosensors and of a multiprotein nanovesicular biomimetic to study the processes of initiation of skeletal mineralization.

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Section: New Perspectives For Applications Of Proteoliposomesmentioning

confidence: 99%

Proteoliposomes in nanobiotechnology

et al. 2012

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“…The proteoliposome system provides a means of reconstituting lipid vesicles that will function like MVs. There have been previous attempts to simulate MV mineralization using multilamellar liposomal systems (44,45,56,57). More recently, liposomes containing various proportions of anionic and neutral phospholipids and cholesterol were used to study the ability of annexin V to facilitate Ca 2+ uptake (26).…”

Section: Liposome Systemsmentioning

confidence: 99%

Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization

Simão

Yadav

Ciancaglini

et al. 2010

Braz J Med Biol Res

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix by promoting the formation of hydroxyapatite (HA) seed crystals in the sheltered interior of membrane-limited matrix vesicles (MVs). Ion transporters control the availability of phosphate and calcium needed for HA deposition. The lipidic microenvironment in which MV-associated enzymes and transporters function plays a crucial physiological role and must be taken into account when attempting to elucidate their interplay during the initiation of biomineralization. In this short mini-review, we discuss the potential use of proteoliposome systems as chondrocyte- and osteoblast-derived MVs biomimetics, as a means of reconstituting a phospholipid microenvironment in a manner that recapitulates the native functional MV microenvironment. Such a system can be used to elucidate the interplay of MV enzymes during catalysis of biomineralization substrates and in modulating in vitro calcification. As such, the enzymatic defects associated with disease-causing mutations in MV enzymes could be studied in an artificial vesicular environment that better mimics their in vivo biological milieu. These artificial systems could also be used for the screening of small molecule compounds able to modulate the activity of MV enzymes for potential therapeutic uses. Such a nanovesicular system could also prove useful for the repair/treatment of craniofacial and other skeletal defects and to facilitate the mineralization of titanium-based tooth implants.

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“…In studying the effect of membrane-bound acidic phospholipids on calcium phosphate (CapI) precipitation, we have used as our basic membrane model liposomes prepared from mixtures of phosphatidylcholine, dicetyl phosphate, and cholesterol, usually in the molar ratios of 7:l-2:l (Eanes et al, 1984). Although dissimilar in composition to matrix vesicle membranes, this specific mixture was chosen because the liposomes formed stable aqueous suspensions that did not readily fuse in the presence of solution Ca2+.…”

Section: Liposome Preparationmentioning

confidence: 99%

“…As detailed elsewhere (Eanes et al, 1984), the first step in the preparation of the liposomes was rotatory evaporating from chloroform the primary lipid mixture as a thin film on the inner surface of a round bottom flask. Where appropriate, the primary mixture was augmented with up to 20 mole % of one of the following acidic phospholipids: phosphatidic acid, phosphatidylglycerol, phosphatidylinositol, or phosphatidylserine.…”

Section: Liposome Preparationmentioning

confidence: 99%

“…As stated above, phosphatidylcho1ine:dicetyl phosphate:cholesterol liposomes did not induce precipitation when suspended in metastable CapI solutions. However, precipitates readily formed when the aqueous interiors of the liposomes were filled with buffered 25-50 mM phosphate solutions and the membrane made permeable to external Ca2+ ions with cationophores such as lasalocid acid (X-537A) (Eanes et al, 1984) (Fig. 1).…”

Section: Calcium Phosphate Precipitation In Phosphatidylch0line:dlcetmentioning

confidence: 99%

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Biophysical aspects of lipid interaction with mineral: Liposome model studies

Eanes

1989

Anat. Rec.

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The present paper reviews the use of liposomes as synthetic models for studying various biophysical aspects of matrix vesicle calcification, especially the involvement of acidic phospholipids in the nucleation and growth processes which occur during the initial stages of mineral formation in and around these membrane-bound structures. Recent results showed that acidic phospholipids incorporated into phosphatidylcholine-rich anionic liposome membranes were ineffective in initiating extraliposomal calcium phosphate precipitation from metastable solutions at physiological pH. On the contrary, certain acidic phospholipids such as phosphatidic acid and phosphatidylserine retarded the development of such precipitation when the latter was endogenously induced. The extent of inhibition correlated with the strength of the electrostatic interaction between the polar head group of the acidic phospholipid and the surface of the mineral phase. The results suggest that acidic phospholipids may play an important role in controlling the rate of early mineral development in matrix vesicle calcification.

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Calcium phosphate formation in aqueous suspensions of multilamellar liposomes

Cited by 44 publications

References 32 publications

Proteoliposomes in nanobiotechnology

Proteoliposomes in nanobiotechnology

Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization

Biophysical aspects of lipid interaction with mineral: Liposome model studies

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