Calcium carbonate prevents Botryococcus braunii growth inhibition caused by medium acidification

Miura, Ryoji; Furuhashi, Kenichi; Hasegawa, Fumio; Kaizu, Yutaka; Imou, Kenji

doi:10.1007/s10811-021-02622-w

Cited by 3 publications

(5 citation statements)

References 31 publications

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“…For the insoluble IC (MgCO 3 or CaCO 3 ) groups, the increased pH also suggested the partial solubilization of carbonate into the medium in the form of soluble bicarbonate as a carbon source for Tetraselmis subcordiformis. Similarly, the leach of Ca 2+ into the medium was recorded in the Botryococcus braunii culture with CaCO 3 addition for the photoautotrophic NH 4 + -N removal [17]. The release of H + during the NH 4 + -N assimilation could be the trigger for this solubilization.…”

Section: Ph Variation Photosynthetic Performance and Cell Growthmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

“…Aside from the soluble bicarbonate NaHCO 3 , insoluble carbonates such as CaCO 3 have been shown to efficiently regulate pH for microalgae cultivated in NH 4 + -rich wastewaters [17,18]. The insoluble nature of CaCO 3 restricted the unnecessary increase in pH [17]. However, since Ca 2+ released into the water with the NH 4 + removal process can be inhibitory to microalgae [19], and the insoluble particles can shelter the microalgae from being exposed to light that is crucial for photosynthesis, the ultimate performance of the insoluble carbonate needs to be evaluated for comparison with traditional NaHCO 3 .…”

Section: Introductionmentioning

confidence: 99%

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CO2-Inorganic Carbon Auto-Buffering System for Efficient Ammonium Reclamation Coupled with Valuable Biomass Production in a Euryhaline Microalga Tetraselmis subcordiformis

Shen

Liao

et al. 2023

Water

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The performance of microalgae-based wastewater treatment processes for ammonium-N (NH4+-N) removal depends on the maintenance of a favorable pH that is critical for minimizing nitrogen escape in the form of free ammonia (NH3) and preventing high-NH3 or extreme-pH stress. This study developed a CO2-inorganic carbon (CO2-IC) buffering system that automatically stabilized pH with the supply of a carbon source for efficient photosynthetic reclamation of NH4+-N by a euryhaline microalga Tetraselmis subcordiformis. The soluble (NaHCO3) and insoluble (CaCO3 and MgCO3) ICs were compared for this purpose. The pH was well controlled in the range of 6.5~8.5 in the CO2-IC system, which was suitable for the photosynthetic growth of T. subcordiformis. The NH4+-N (100 mg/L) was almost completely removed in three days, with the maximum removal rate of 60.13 mg N/L/day and minimal N escape of 19.65% obtained in the CO2-NaHCO3 system. The CO2-IC system also restricted the release of extracellular organic matter by preventing stress conditions. The CO2-NaHCO3 system enabled the highest “normal” starch production suitable for fermentation, while the CO2-CaCO3/MgCO3 system facilitated high-amylose starch accumulation that was conducive to producing bio-based materials and health-promoting ingredients. The proteins accumulated in T. subcordiformis were of good quality for animal feeds.

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Section: Ph Variation Photosynthetic Performance and Cell Growthmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CO2-Inorganic Carbon Auto-Buffering System for Efficient Ammonium Reclamation Coupled with Valuable Biomass Production in a Euryhaline Microalga Tetraselmis subcordiformis

Shen

Liao

et al. 2023

Water

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“…Therefore, in the CE medium, decreasing pH and growth were observed due to excess H+ release in the assimilation of NH4 + 12) . This pH decrease can be easily adjusted by using crushed calcium carbonate 15) .…”

Section: Application Of Compost Extract As Liquid Fertilizer For Leaf...mentioning

confidence: 99%

“…In our previous research, we developed a method to avoid growth inhibition of microalgae due to acidification, which can be easily introduced in large-scale culture by adding calcium carbonate into the medium 15) . Among the microalgae with the potential to be used as biofuel and health food materials, the green colonial alga Botryococcus braunii (B race) is known to produce and accumulate more than 30% of C30-34 hydrocarbons per dry matter in its extracellular hydrophobic structures as it grows 16) 17) .…”

Section: Introductionmentioning

confidence: 99%

Production and Application of High-nitrogen Compost Extract as Liquid Fertilizer for Leafy Vegetable and Microalgae

Furuhashi

Hasegawa

Tanaka

et al. 2022

J. Jpn. Inst. Energy

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This study aimed to produce high-nitrogen compost extract and applicate it to land plants and microalgae, a candidate crop for biofuel and high value-added material production, as a part of low-salinity compost production system. For this purpose, we selected komatsuna, a leafy vegetable, and Botryococcus braunii, a microalga, as the test species. Nitrogen-added compost, which was used as a deodorizing material for ammonia released from composting, had a higher amount of inorganic nitrogen than potassium, unlike general dairy cattle manure compost.The extraction efficiency of the fertilizer elements from nitrogen-added compost did not increase by extending the extraction time (5 min) or increasing the solvent ratio to 4, except for phosphoric acid. That of phosphoric acid increased with the solvent ratio up to 100. The moisture content discharged from screw press tended to be lower than that of the dairy manure slurry. In the culture of komatsuna, compost extract could be used as an alternative to chemical fertilizers by supplementing the deficient of phosphoric acid. In the microalgae, compost extracts could be also used as an alternative to chemical fertilizers although phosphorus deficiency was observed in the late stage.

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Integration of pre-precipitation optimizing performance of culture medium prepared from salvaged cyanobacterial slurry

et al. 2023

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Calcium carbonate prevents Botryococcus braunii growth inhibition caused by medium acidification

Cited by 3 publications

References 31 publications

CO2-Inorganic Carbon Auto-Buffering System for Efficient Ammonium Reclamation Coupled with Valuable Biomass Production in a Euryhaline Microalga Tetraselmis subcordiformis

CO2-Inorganic Carbon Auto-Buffering System for Efficient Ammonium Reclamation Coupled with Valuable Biomass Production in a Euryhaline Microalga Tetraselmis subcordiformis

Production and Application of High-nitrogen Compost Extract as Liquid Fertilizer for Leafy Vegetable and Microalgae

Integration of pre-precipitation optimizing performance of culture medium prepared from salvaged cyanobacterial slurry

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