“…25 A potential drawback to using a relative nonspecific enzyme, such as alkaline phosphatase, is that it can also enzymatically degrade other phosphate-containing compounds, such as ADP, an important platelet agonist. And finally, the isolated polyP binding domain of E coli exopolyphosphatase has been used successfully as a probe to localize polyP in yeast cells 107 and in acidocalcisomes in eggs of sea urchins 108 and insects. 109 For a variety of experiments, it would be desirable to be able to covalently attach biotin, epitope tags, dyes, fluorophores, etc, to polyP, and also to covalently immobilize polyP onto solid supports, such as magnetic beads and multiwell plates.…”