Calcium and oestrogen interactions upon the rat thymic lymphocyte plasma membrane

Moran, Jill; Bramhall, John; Britten, A. Z.; Perris, A. D.

doi:10.1016/s0006-291x(76)80091-5

Cited by 10 publications

(2 citation statements)

References 16 publications

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“…The suppression of the response to elevated Ca 2÷ of female lymphocytes has been attributed to the presence, in vivo, of estradiol-17~, a steroid that also blocks calcium induced mitogenesis in vitro (Morgan and Perris, 1975). Morgan et al (1976) showed that the membrane potential hyperpolarized upon the addition of extracellular Ca 2., with the maximal effect occurring at about 1.8 mM. (Magnesium strontium, zinc, manganese, and tin had no effect.)…”

Section: Lymphocytes and Plateletsmentioning

confidence: 99%

Optical measurement of membrane potential

Cohen

Salzberg

1978

Reviews of Physiology, Biochemistry and Pharmacology

483

285

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Section: Lymphocytes and Plateletsmentioning

confidence: 99%

Optical measurement of membrane potential

Cohen

Salzberg

1978

Reviews of Physiology, Biochemistry and Pharmacology

483

285

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“…Sims et al (60) studied the spectra and the cellular uptake and distribution of a series of symmetric cyanine dyes which have been used extensively (5, 6, 27,31,36,37,42,44,50,55) for measurement of membrane potential in suspensions of cells, organelles, and liposomes. These authors also introduced the shorthand notation "DiYC,(2m + 1)" for the symmetric cyanine dye structure shown in Figure 1.…”

Section: Measurement Of Membrane Potentialmentioning

confidence: 99%

Flow cytometric probes of early events in cell activation

Shapiro

1981

Cytometry

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By now, it's well established that the surface of a cell Contains transmembrane proteins which, on binding ligands, tell The biochemical computer inside what to do Without a need for any of the ligands coming through. Receptor‐triggered cytoplasmic “interrupt routines” May lead the nucleus to activate new sets of genes, Or otherwise induce a cell, which never reasons why, To twitch, secrete, endocytose, or reproduce, or die. By merely watching labeled ligands bind, we cannot know Down which of many paths the cells thus tagged are apt to go, But other changes, shortly after binding, may predict Which of its several options any given cell has picked. Flow cytometric methods are described here which allow Membrane potential to be estimated, to show how Potential changes may occur in seconds after binding Of ligand to a functional receptor, or for finding No change when cells don't have the right receptor, or can't see The ligand for antagonists of like affinity. With chlorotetracycline fluorescence, one can find Release of membrane calcium soon after ligands bind; Both this and the potential measurement can help support Decisions in an instrument about which cells to sort, Though narrowing the distributions which these methods yield Would greatly help to broaden applications in this field.

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