Caged ceramide 1-phosphate (C1P) analogs: Novel tools for studying C1P biology

Gómez-Muñoz, Antonio; Gangoiti, Patricia; Rivera, Io-Guané; Presa, Natalia; Gómez-Larrauri, Ana; Ordoñez, Marta

doi:10.1016/j.chemphyslip.2015.07.019

Cited by 15 publications

(6 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Light induced cleavage of the protective group liberates the active lipid to achieve its necessary intracellular concentration. While a variety of caged lipid analogs have been synthesized in several major classes (PA, PIs, PEs, sphingolipids, ceramides and ceramide 1-phosphate, triacylglycerols), , no caged CLs have been reported so far. It is tempting to speculate that caged CLs and CLox could be instrumental to the identification of the mechanisms through which they facilitate the execution of apoptotic cell death.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial Redox Opto-Lipidomics Reveals Mono-Oxygenated Cardiolipins as Pro-Apoptotic Death Signals

Mao¹,

Qu²,

Croix³

et al. 2016

ACS Chem. Biol.

View full text Add to dashboard Cite

While opto-genetics has proven to have tremendous value in revealing the functions of the macromolecular machinery in cells, it is not amenable to exploration of small molecules such as phospholipids (PLs). Here, we describe a redox opto-lipidomics approach based on a combination of high affinity light-sensitive ligands to specific PLs in mitochondria with LC-MS based redox lipidomics/bioinformatics analysis for the characterization of pro-apoptotic lipid signals. We identified the formation of mono-oxygenated derivatives of C18:2-containing cardiolipins (CLs) in mitochondria after the exposure of 10-nonylacridine orange bromide (NAO)-loaded cells to light. We ascertained that these signals emerge as an immediate opto-lipidomics response, but they decay long before the commencement of apoptotic cell death. We found that a protonophoric uncoupler caused depolarization of mitochondria and prevented the mitochondrial accumulation of NAO, inhibited the formation of C18:2-CL oxidation product,s and protected cells from death. Redox opto-lipidomics extends the power of opto-biologic protocols to studies of small PL molecules resilient to opto-genetic manipulations.

show abstract

Section: Resultsmentioning

confidence: 99%

Mitochondrial Redox Opto-Lipidomics Reveals Mono-Oxygenated Cardiolipins as Pro-Apoptotic Death Signals

Mao¹,

Qu²,

Croix³

et al. 2016

ACS Chem. Biol.

View full text Add to dashboard Cite

show abstract

“…On the contrary, several studies performed in regions characterized by mild to moderate iodine deficiency or even sufficient iodine intake showed a positive correlation between TSH level and blood pressure values (106, 119). The differences observed in the results, however, may be partially due to the higher prevalence of hypertension in Far East Asia compared to Europe and the alternate screening and treatment methods used in these regions (120).…”

Section: Blood Pressure Changes In Subclinical Hypothyroidismmentioning

confidence: 99%

Hypertension in Thyroid Disorders

et al. 2019

View full text Add to dashboard Cite

Arterial hypertension represents a major global health concern; more than one fourth of the population is affected by high blood pressure. Albeit the underlying cause of the disease remains unclear in the vast majority of the cases, ~10% are of secondary origin. Endocrine disorders are common illnesses and some of them may lead to elevated blood pressure, among which thyroid diseases are of high prevalence and often overlooked, especially in mild cases. Overt and subclinical hyper- and hypothyroidism can both lead to (mostly mild) hypertension; however, the underlying mechanisms are only partially understood. The results of clinical studies are often controversial. During the past decades, some genetic mutations in the hypothalamus-pituitary-thyroid axis with cardiovascular consequences were revealed. Atherosclerotic changes resulting from lipid abnormalities due to thyroid dysfunction also affect the vasculature and can cause elevated blood pressure. The review gives a synopsis of our knowledge how thyroid hormone metabolism and functional thyroid diseases affect the cardiovascular system, their negative impact and causative role in the development of hypertension.

show abstract

“…Furthermore, we have also searched for a number of other signalling lipids, including sphingolipid metabolites, monoacylglycerols (MAGs), and dihydroxy-derivates of docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA). Of the sphingolipid derivates, both sphingosine-1-phosphate (S1P) as an intercellular messenger28 and ceramide-1-phosphate (CerP), whether extracellular or intracellular29, have been implicated in regulation of neuronal damage3031. The endocannabinoid, 2-arachidonoylglycerol (2-AG), is an important neuromodulator in the brain in both normal physiology32 as well as during brain injury33, but also as a precursor for eicosanoids during neuroinflammation34.…”

mentioning

confidence: 99%

Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia

Nielsen

Lambertsen

Clausen

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively.

show abstract

Caged ceramide 1-phosphate (C1P) analogs: Novel tools for studying C1P biology

Cited by 15 publications

References 57 publications

Mitochondrial Redox Opto-Lipidomics Reveals Mono-Oxygenated Cardiolipins as Pro-Apoptotic Death Signals

Mitochondrial Redox Opto-Lipidomics Reveals Mono-Oxygenated Cardiolipins as Pro-Apoptotic Death Signals

Hypertension in Thyroid Disorders

Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia

Contact Info

Product

Resources

About