Caffeic acid product from the highly copper-tolerant plant Elsholtzia splendens post-phytoremediation: its extraction, purification, and identification

Abstract

In the current study, caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens. Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction, followed by purification using a macroporous resin (D101 type) column and silica gel chromatography. The faint-yellow caffeic acid product was yielded with a purity of 98.46%, and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance ( 1 H NMR)/carbon nuclear magnetic resonance ( 13 C NMR), and electrospray ionization mass spectrometry (ESI-MS). Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.

Caffeic acid production enhancement by engineering a phenylalanine over‐producing Escherichia coli strain

“…Despite its established market and hopeful expansion of applications, current production approaches of caffeic acid from plant extract have been regarded as energy‐intensive and environmentally unfriendly (Xing et al, ). And it is desirable to develop more efficient innovative producing strategies to meet the growing market and to lower the cost.…”

A voltammetric determination of caffeic acid in red wines based on the nitrogen doped carbon modified glassy carbon electrode

“…Moreover, the determination of CA in wines received a considerable importance in quality control analysis. Hitherto, various analytical methods have been developed to determine the CA, including capillary gas chromatographic method8, liquid chromatography–electro spray ionization mass spectrometry9, high pressure liquid chromatography (HPLC)1011, capillary electrophoresis12 and voltammetric method13. These reported methods need sophisticated facilities and highly skilled technicians to operate those instruments, furthermore, they are time consuming process.…”

Heterologous production of caffeic acid from tyrosine in Escherichia coli

“…ethyl acetate), thus being energy-intensive and environmentally unfriendly [7]. Moreover, 72 the yields obtained are low because it accumulates at low levels in plant cells.…”