Cadmium speciation in Arabidopsis thaliana as a strategy to study metal accumulation system in plants

Pawlak, Katarzyna; Ruzik, Lena; Abramski, Krzysztof M.; Ciurzynska, M; Gawrońska, H.

doi:10.1016/j.aca.2004.10.048

Cited by 63 publications

(50 citation statements)

References 27 publications

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“…The mobile phase is determined by assessing sample-solvent solubility and its chemical compatibility with the stationary phase [87]. As shown in Table 1, mobile phases commonly employed in SEC techniques are Tris-HCL buffer [9], Tris buffer with NaCl [89], ammonium acetate [4; 90; 91] and sodium borohydride [92].…”

Section: Size Exclusion Chromatography (Sec)mentioning

confidence: 99%

“…Cadmium commonly enters the environment from the burning of fossil fuels and municipal wastes [114] and is carcinogenic, teratogenic and mutagenic in high concentrations [89]. Its speciation in plants has been investigated both in terms of analytical method development [9] and translocation mechanisms in the plant itself [115].…”

Section: Cadmiummentioning

confidence: 99%

“…Cysteine and gluthathione metal complexes were below detection limits. A study by Połed-Pawlak et al [89] found that 89 % of cadmium accumulated in the roots and inhibited growth of root hairs. Furthermore, this study found that the majority of the accumulated cadmium was found within the water-soluble peptide fraction [89].…”

Section: Cadmiummentioning

confidence: 99%

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Metal and metalloid speciation in plants: Overview, instrumentation, approaches and commonly assessed elements

Kroukamp

Wondimu

Forbes

2016

TrAC Trends in Analytical Chemistry

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The ability of plants to hyper-accumulate metals and metalloids from the surrounding environment may pose a significant health risk to both humans and animals since plants form a substantial component of diet. This attribute, however, has also been identified as a useful tool in bioremediation and biomonitoring studies; where assimilated metal(loid)s in plants often correlate to environmental exposure. Since the bioavailability and toxicity of these elements depends upon their chemical form, speciation studies are essential in determining mobility and metabolic pathways. This can be done in a number of ways where sampling, pre-treatment and storage are all important factors affecting speciation. Appropriate analytical techniques for speciation studies can either be direct methods such as XAS, or indirect methods which require species separation prior to analysis. Separation techniques can be in the form of sequential extractions or column separation and analyte detection often utilises instrumentation such as ESI-MS, ICP-MS and ICP-OES.

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Section: Size Exclusion Chromatography (Sec)mentioning

confidence: 99%

Section: Cadmiummentioning

confidence: 99%

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Metal and metalloid speciation in plants: Overview, instrumentation, approaches and commonly assessed elements

Kroukamp

Wondimu

Forbes

2016

TrAC Trends in Analytical Chemistry

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“…Cadmium is a heavy metal widely released to the environment by power stations, heating systems, metal working industries, waste incinerators, urban traffic, cement factories, phosphate fertilizer factories and other sources [6]. Since cadmium is a relatively immobile element (except in acidic soil), it can accumulate in soil and become dangerous to living systems.…”

Section: Introductionmentioning

confidence: 99%

An HPLC-ICP-MS technique for determination of cadmium–phytochelatins in genetically modified Arabidopsis thaliana

Sadi

Vonderheide

Gong

et al. 2008

Journal of Chromatography B

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A reversed-phase high-performance liquid chromatographic technique was developed to separate cadmium-phytochelatin complexes (Cd-PC 2 , Cd-PC 3 , and Cd-PC 4 ) of interest in the plant Arapidopsis thaliana. High-performance liquid chromatography (HPLC) was coupled to an inductively coupled plasma mass spectrometric (ICP-MS) system with some modification to the interface. This was done in order to sustain the plasma with optimum sensitivity for cadmium detection in the presence of the high methanol loads used in the gradient elution of the reversedphase separation. The detection limits were found to be 91.8 ng l −1 , 77.2 ng l −1 and 49.2 ng l −1 for Cd-PC 2 , Cd-PC 3 , and Cd-PC 4 respectively. The regression coefficients (r 2 ) for Cd-PC 2 to Cd-PC 4 detection ranged from 0.998 to 0.999. The method was then used to investigate the occurrence and effect of cadmium-phytochelatin complexes in wild-type Arabidopsis and a phytochelatin-deficient mutant cad1-3 that had been genetically modified to ectopically express the wheat TaPCS1 phytochelatin synthase enzyme. The primary complex found in both wild-type and transgenic plants was Cd-PC 2 . In both lines, higher levels of Cd-PC 2 were found in shoots than in roots, showing that phytochelatin synthases contribute to the accumulation of cadmium in shoots, in the Cd-PC 2 form. Genetic modification did, however, impact the overall accumulation of Cd. Transgenic plants contained almost two times more cadmium in the form of Cd-PC 2 in their roots than did the corresponding wild-type plants. Similarly, the shoot samples of the modified species also contained more (by 1.6 times) cadmium in the form of Cd-PC 2 than the wild type. The enhanced role of PC 2 in the transgenic Arabidopsis correlates with data showing long-distance transport of Cd in transgenic plants. Targeted transgenic expression of non-native phytochelatin synthases may contribute to improving the efficiency of plants for phytoremediation.

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“…Aliquots of 400 mg (n = 3) of each composite plant sample were used for organic complexes extraction in three sequential steps, according to Połéc-Pawlak et al 15 The extraction procedure consisted basically of three steps: (i) (E1) -extraction with 10 mmol L -1 TRIS-HCl (pH 7.4) for aqueous complexes removal located at cytoplasm and vacuoles; (ii) (E2) -extraction with 1% (m/v) sodium dodecil Vol. 22, No.…”

Section: Extraction Procedures and Efficiencymentioning

confidence: 99%

Determination of arsenic species in P. calomelanos and N. biserrata

Melendez¹,

Silva-Filho²,

Miekeley³

et al. 2011

J. Braz. Chem. Soc.

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O sítio industrial abandonado na Baía de Sepetiba (SE-Brasil) está entre os maiores passivos ambientais contaminados com Zn no mundo e o As também se encontra em concentrações alarmantes. A vegetação no local é escassa e das espécies que sobrevivem no local Pityrogramma calomelanos e Nephrolepis biserrata são as mais abundantes. Concentrações de As de até 2271 mg kg -1 foram detectadas na espécie Pityrogramma calomelanos, sendo que essa espécie foi mais eficiente que N. Bisserata no acúmulo desse elemento.Utilizou-se cromatografia de exclusão por tamanho (SEC) com detecção UV-Vis e espectrometria de massas com plasma indutivamente acoplado (ICP-MS) em sistema "on line" para monitorar a distribuição de As na fração orgânica. As maiores concentrações de As foram determinadas na fração extraída com TRIS-HCl, pH 7,4 (entre 60-100% da concentração total de As) mostrando uma evidente associação desse elemento com bioligantes, entre eles, fitoquelatinas e glutationa.The abandoned industrial site at the Sepetiba Bay (SE-Brazil) is one of the most heavily contaminated sites on the earth's surface concerned to Zn, but also presents As in alarming concentrations. Vegetation on this site is scarce and under the surviving species Pityrogramma calomelanos and Nephrolepis biserrata are the most abundant ones. Total As concentrations up to 2271 mg kg -1 in P. calomelanos were determined, being this specie more efficient in arsenic accumulation than N. bisserata. The size exclusion chromatography (SEC) with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (ICP-MS) was used to screen the distribution of As between different molar mass fractions. Highest concentration of As was found after extraction with TRIS-HCl, pH 7.4 (about 60-100% of total As) characterizing an association of this element with bioligands including phytochelatins and glutathione.

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Cadmium speciation in Arabidopsis thaliana as a strategy to study metal accumulation system in plants

Cited by 63 publications

References 27 publications

Metal and metalloid speciation in plants: Overview, instrumentation, approaches and commonly assessed elements

Metal and metalloid speciation in plants: Overview, instrumentation, approaches and commonly assessed elements

An HPLC-ICP-MS technique for determination of cadmium–phytochelatins in genetically modified Arabidopsis thaliana

Determination of arsenic species in P. calomelanos and N. biserrata

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