Cadmium-Induced Testicular Toxicity in Mice: Subacute and Subchronic Route-Dependent Effects

Mouro, Viviane Gorete Silveira; Siman, V. A.; Silva, Janaína; Dias, Fernanda Carolina Ribeiro; Damasceno, E. M.; Cupertino, Marli do Carmo; Melo, Fabiana Cristina Silveira Alves de; Matta, Sérgio Luís Pinto da

doi:10.1007/s12011-019-01731-5

Cited by 37 publications

(19 citation statements)

References 68 publications

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“…3B) compared with the control group, these results in accordance with the previous results of Saha et al (2019). Cd testicular impairment shown in the current study may result from the excess generation of ROS and the reduction of many minerals essential for the occurrence and maintenance of spermatogenesis (Cupertino et al, 2017;Mouro et al, 2019). Among the minerals reduced due to Cd exposure is Zn, which is essential for the production of testosterone (Baltaci et al, 2019).…”

Section: Resultssupporting

confidence: 92%

Protective Effect of Graviola (Annona muricata) Leaves Extract Against Cadmium-Induced Testicular Toxicity In Male Albino Rats

El-Monem

Elwakeel

2020

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Graviola (Annona muricata), an edible plant belonging to the Annonaceae family, has a long history in traditional medicine due to its wide range of therapeutic effects on many ailments. This study was planned to assess the protective potentials of Graviola leaves extract (GLE) on cadmiuminduced testicular damage in male albino rats. The study was conducted on 32 male adult Wistar rats randomly divided into four groups (n=8/each). A (control group); B (GLE, 100 mg/kg); C (cadmium chloride (CdCl 2), 5 mg/kg) and D (GLE, 100 mg / kg + CdCl 2 5 mg/kg). All administrations were performed by daily gavage for 8 weeks. Blood, testes, and epididymis were harvested for biochemical and histological investigations. In addition to sperm analysis, sperm DNA comet assay, and apoptotic indices. Results revealed that GLE significantly reversed (p<0.05) testicular oxidative stress induced by CdCl 2 , maintained male sex hormones levels, and restored testis architecture via increasing the testicular antioxidant status. GLE co-treatment significantly increased (p<0.05) sperm count, motility, viability, and DNA integrity along with significantly reducing sperm DNA damage and sperm head abnormality in comparison with CdCl 2 group. Additionally, GLE protected germ cells from Cd-induced apoptosis. In conclusion, results of the present study confirmed that GLE has potent antioxidant potentials and cytoprotective capacity against the deleterious effects of CdCl 2 on testicular tissue by reducing cellular oxidative stress prompted by the generation of the reactive oxygen species.

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Section: Resultssupporting

confidence: 92%

Protective Effect of Graviola (Annona muricata) Leaves Extract Against Cadmium-Induced Testicular Toxicity In Male Albino Rats

El-Monem

Elwakeel

2020

Egyptian Academic Journal of Biological Sciences, B. Zoology

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“…Then, the samples were diluted in deionized water in a 10 mL volumetric flask and filtered using filter papers. The concentrations of cadmium (Cd), zinc (Zn), calcium (Ca), magnesium (Mn), manganese (Mg), copper (Cu), and iron (Fe) were determined using an atomic absorption spectrophotometer (SpectrAA 220FS Varian) [14].…”

Section: Methodsmentioning

confidence: 99%

“…The area results were expressed as square millimeter (mm 2 ). The tubule epithelium ratio (TER) was obtained by dividing the STAr by the EAr [14].…”

Section: Methodsmentioning

confidence: 99%

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Subacute Testicular Toxicity to Cadmium Exposure Intraperitoneally and Orally

Mouro

Martins

Silva

et al. 2019

Oxidative Medicine and Cellular Longevity

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The toxic effects of cadmium (Cd) on reproductive parameters are widely described in the literature. Experimental models often make use of the intraperitoneal route (i.p.), although human intoxication occurs preferentially by the oral route and can be continuous. However, little is known about the effect of Cd administration routes on the testicular structure. Thus, this study investigated the testicular impact of Cd exposure comparing both i.p. and oral routes, both single dose (SD), in addition to the oral route in fractional doses (FD). Swiss adult male mice received CdCl2 1.5 mg/kg i.p., 30 mg/kg oral SD, and 4.28 mg/kg oral FD for 7 consecutive days. The Cd bioaccumulation was observed in all routes, mainly in the oral FD route. The concentrations of testicular Ca and Cu decreased in all animals exposed to Cd, while Zn and Mn decreased only in the i.p. route. Testicular SOD activity was reduced in both routes of oral administration, while CAT increased in the i.p. route, and GST increased in all animals exposed to Cd. Changes in the tubular parameters and cell viability were observed in both routes of Cd administration but were more intense in the oral route, mainly in the FD. Serum testosterone concentration was reduced in both routes of oral administration. Tubular damage, such as the vacuolization of the seminiferous epithelium, germ cell detachment, and seminiferous tubule degeneration, occurred in all groups exposed to Cd. Therefore, the oral Cd administration presented greater potential to promote testicular damage, mainly when the metal was given in a fractionated way.

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“…Efforts have been made to understand the exact mechanisms of toxicity of Cd and available body of knowledge suggests oxidative stress as one of the critical mechanism of toxicity of this metal, albeit the metal is a Fenton’s metal [ [6] , [7] , [8] ]. Other possible mechanisms of toxicity include binding to oxygen, nitrogen, and sulphur ligands, which may affect numerous enzymes and proteins; interaction with bio-elements; inhibition of apoptosis and changes in DNA structure and the inhibition of damaged DNA repair, which may lead to aberrant gene expression [ 2 , 9 , 10 ]. Additionally, Cd has been shown to exert toxicity in the hippocampus of the brain [ 11 ], prostate, pancreas, endocrine system and the heart causing memory defects, cardiovascular dysfunctions, immune and reproductive system damages [ [12] , [13] , [14] , [15] ].…”

Section: Introductionmentioning

confidence: 99%

Hesperidin ameliorates hepatic dysfunction and dyslipidemia in male Wistar rats exposed to cadmium chloride

et al. 2020

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Cadmium-Induced Testicular Toxicity in Mice: Subacute and Subchronic Route-Dependent Effects

Cited by 37 publications

References 68 publications

Protective Effect of Graviola (Annona muricata) Leaves Extract Against Cadmium-Induced Testicular Toxicity In Male Albino Rats

Protective Effect of Graviola (Annona muricata) Leaves Extract Against Cadmium-Induced Testicular Toxicity In Male Albino Rats

Subacute Testicular Toxicity to Cadmium Exposure Intraperitoneally and Orally

Hesperidin ameliorates hepatic dysfunction and dyslipidemia in male Wistar rats exposed to cadmium chloride

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