Cadmium induced BEAS-2B cells apoptosis and mitochondria damage via MAPK signaling pathway

Cao, Xiangyu; Fu, Mingyang; Bi, Ruochen; Zheng, Xiaoliang; Fu, Baorong; Tian, Siqi; Liu, Chengying; Li, Qijiu; Liu, Jianli

doi:10.1016/j.chemosphere.2020.128346

Cited by 160 publications

(84 citation statements)

References 57 publications

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“…Among them, the problem of Cd exceeding the standard is a major problem affecting the food safety of A. brasiliensis industry. Heavy metal stress may be caused by oxidative stress, DNA damage, inhibition of enzyme function and destruction of protein functions that regulate cell proliferation, differentiation or apoptosis 25 . A series of changes in agronomic characters of A. brasiliensis were observed under Cd stress, which indicated that low concentration of Cd could promote the proliferation and differentiation of A. brasiliensis cells, and when the concentration was high, the cell apoptosis would be inhibited 26 .…”

Section: Discussionmentioning

confidence: 99%

Effects of cadmium stress on physiological indexes and fruiting body nutritions of Agaricus brasiliensis

Liu

Cai

Yuan

et al. 2021

Sci Rep

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In this study, 0, 0.5, 1, 1.5, 2, 4, 6 and 8 mg·kg−1 of cadmium were added to the cultivation materials. In order to study the effects of different concentrations of Cd stress on J1 and J77, the contents of antioxidant enzymes, proline and malondialdehyde, Cd content, agronomic traits and yield of fruiting bodies of Agaricus brasiliensis were determined, and the nutritional components such as polysaccharide, triterpene, protein, total sugar and total amino acid were determined. The results showed that the physiological indexes of strain J1 and J77 changed regularly under different concentrations of Cd stress. J1 was a high absorption and low tolerance variety, while J77 was a low absorption and high tolerance variety. Low concentration of Cd promoted the growth of strain J1, and higher concentration of Cd promoted the growth of strain J77. The contents of protein and total amino acids in the two strains changed greatly, followed by polysaccharides, which indicated that Cd stress had the greatest impact on the three nutrients, and other nutrients were not sensitive to Cd stress.

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Section: Discussionmentioning

confidence: 99%

Effects of cadmium stress on physiological indexes and fruiting body nutritions of Agaricus brasiliensis

Liu

Cai

Yuan

et al. 2021

Sci Rep

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“…In the present study we used Nthy-ori-3-1 cells, widely used as preclinical model of non-tumorigenic human thyrocytes, to analyze the molecular alterations caused by CdCl 2 involved in its toxic activity, focusing our attention on the alterations of cellular redox status regulation. Our findings demonstrate that CdCl 2 suppresses cell growth in a dose-and time-dependent manner and is associated with a decrease in the levels of phospho-ERK, suggesting the involvement of the MAPKmediated pathway, a well-established transduction pathway of regulation of thyrocyte proliferation [19], and indicated as a target of Cd toxic action even in other cell types [20,21]. In addition, in our experimental model, CdCl 2 elicited an increase in ROS levels, lipid peroxidation and ER stress, suggesting that the toxic effect of Cd in thyroid cells may be determined by the production of free radicals which, by stimulating lipid peroxidation in biomembranes, lead to cell damage, as also documented by the up-regulation of GRP78, a regulator of ER stress.…”

Section: Discussionmentioning

confidence: 64%

Quercetin Protects Human Thyroid Cells against Cadmium Toxicity

Capriglione

Maiuolo

Celano

et al. 2021

IJMS

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Various natural compounds have been successfully tested for preventing or counteracting the toxic effects of exposure to heavy metals. In this study, we analyzed the effects of cadmium chloride (CdCl2) on immortalized, non-tumorigenic thyroid cells Nthy-ori-3-1. We investigated the molecular mechanism underlying its toxic action as well as the potential protective effect of quercetin against CdCl2-induced damage. CdCl2 suppressed cell growth in a dose- and time-dependent manner (IC50 value ~10 μM) associated with a decrease in levels of phospho-ERK. In addition, CdCl2 elicited an increase in reactive oxygen species (ROS) production and lipid peroxidation. A significant increase in GRP78, an endoplasmic reticulum (ER) stress-related protein, was also observed. Supplementation of quercetin counteracted the growth-inhibiting action of CdCl2 by recovering ERK protein phosphorylation levels, attenuating ROS overproduction, decreasing MDA content and reducing the expression of GRP78 in cells exposed to CdCl2. Thus, in addition to revealing the molecular effects involved in cadmium-induced toxicity, the present study demonstrated, for the first time, a protective effect of quercetin against cadmium-induced damages to normal thyroid cells.

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“…The MAPK signaling pathway is closely related to oxidative stress and promotes the process of cell apoptosis, containing the ERK, JNK and p38 MAPK (Ebegboni et al 2019). ROS accumulation can active MAPK pathway and induce oxidative stress (Cao et al 2021). The phosphorylation of ERK, JNK and p38 was upregulated signi cantly after COFs treatment, suggesting that the MAPK signaling pathway in BEAS-2B cells were activated, which was in consistence with the mechanism about the toxicity of copper in mice (Guo et al 2021b).…”

Section: Discussionmentioning

confidence: 99%

“…The proportion of pro-apoptotic members to the level of Bax in mitochondria is closely related to cell survival or apoptosis (Ingo Schmitz 2000). The ratio of Bcl-2 to Bax determines the cell's life and death, Bcl-2 family proteins transfer from the cytoplasm to the mitochondrial membrane and mediate the activation of caspase, mediating the mitochondrial endogenous apoptosis pathway (Cao et al 2021;Yang et al 2019). It has been reported that crotonaldehyde could induce Beas-2B cells apoptosis by regulating the expression of caspase family proteins (Wang et al 2019).…”

Section: Discussionmentioning

confidence: 99%

The Toxicity of Cooking Oil Fumes on Human Bronchial Epithelial Cells Through ROS-Mediated MAPK, NF-κB Signaling Pathways and NLRP3 Inflammasome

Cao¹,

Fu²,

Miao³

et al. 2021

Preprint

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Cooking oil fumes (COFs) are main pollutants in kitchen and indoor air, which threaten human health. Exposure to COFs can cause respiratory diseases and impair pulmonary function. To investigate the toxicity of COFs on human bronchial epithelial cells (Beas-2B) and explore the underlying mechanisms, MTT assay was conducted to detect the viability of Beas-2B. Intracellular reactive oxygen species (ROS) levels and nitric oxide (NO) levels were determined with DCFH-DA assay and DAF-FM assay. The expression of genes involved in inflammation were measured with quantitative real-time PCR (qRT-PCR). The phosphorylation and the expression of proteins related to Mitogen-activated protein kinase (MAPK), NF-κB signaling pathways were measured with western blot. Our results revealed that COFs decreased Beas-2B cells viability, increased the ROS levels and NO levels in cells and induced apoptosis in Beas-2B cells. The results of qRT-PCR and western blot showed that the expression of NLRP3, p65, iNOS, IL-1β, and the factors related to oxidative stress and inflammation increased, NF-κB signaling pathway and MAPK signaling pathway were activated. This study provided some useful information to alleviate the toxicity of COFs and revealed the possible mechanism for the damage on respiratory system induced by COFs.

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Cadmium induced BEAS-2B cells apoptosis and mitochondria damage via MAPK signaling pathway

Cited by 160 publications

References 57 publications

Effects of cadmium stress on physiological indexes and fruiting body nutritions of Agaricus brasiliensis

Effects of cadmium stress on physiological indexes and fruiting body nutritions of Agaricus brasiliensis

Quercetin Protects Human Thyroid Cells against Cadmium Toxicity

The Toxicity of Cooking Oil Fumes on Human Bronchial Epithelial Cells Through ROS-Mediated MAPK, NF-κB Signaling Pathways and NLRP3 Inflammasome

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