Cadmium effect on hydrogen peroxide, gluthatione and phytochelatins levels in potato tuber

Stroiński, Andrzej; Zielezińska, M.

doi:10.1007/s11738-997-0029-2

Cited by 24 publications

(17 citation statements)

References 38 publications

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“…This is most likely due to metal-induced disturbance of AOS-generating metabolism and deactivation of the processes required for AOS destruction (Dietz et al, 1999). Accumulation of hydrogen peroxide (H 2 O 2 ) is a typical response of many plant species to toxic concentrations of Cd (Romero-Puertas et al, 1999;Schützendübel et al, 2001;Stroinski and Zielezinska, 1997). Cd has been found to trigger oxidative burst in tobacco cells within minutes of exposure (Piqueras et al, 1999), possibly signaling the first defenses against heavy metals at the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens

Boominathan

Doran

2003

Biotech & Bioengineering

187

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Plant species capable of hyperaccumulating heavy metals are of considerable interest for phytoremediation and phytomining. This work aims to identify the role of antioxidative metabolism in heavy metal tolerance in the Cd hyperaccumulator, Thlaspi caerulescens. Hairy roots of T. caerulescens and the non-hyperaccumulator, Nicotiana tabacum (tobacco), were used to test the effects of high Cd environments. In the absence of Cd, endogenous activities of catalase were two to three orders of magnitude higher in T. caerulescens than in N. tabacum. T. caerulescens roots also contained significantly higher endogenous superoxide dismutase activity and glutathione concentrations. Exposure to 20 ppm (178 microM) Cd prevented growth of N. tabacum roots and increased hydrogen peroxide (H(2)O(2)) levels by a factor of five relative to cultures without Cd. In contrast, growth was maintained in T. caerulescens, and H(2)O(2) concentrations were controlled to low, nontoxic levels in association with a strong catalase induction response. Treatment of roots with the glutathione synthesis inhibitor, buthionine sulfoximine (BSO), exacerbated H(2)O(2) accumulation in Cd-treated N. tabacum, but had a relatively minor effect on H(2)O(2) levels and did not reduce Cd tolerance in T. caerulescens. Lipid peroxidation was increased by Cd treatment in both the hyperaccumulator and non-hyperaccumulator roots. This work demonstrates that metal-induced oxidative stress occurs in hyperaccumulator tissues even though growth is unaffected by the presence of heavy metals. It also suggests that superior antioxidative defenses, particularly catalase activity, may play an important role in the hyperaccumulator phenotype of T. caerulescens.

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Section: Introductionmentioning

confidence: 99%

Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens

Boominathan

Doran

2003

Biotech & Bioengineering

187

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“…PCs contribute to Cd tolerance in many plants (Steffens et al 1986;Gupta and Goldsbrough 1991;Stroinski and Zielezinska 1997). In the CW and CCdl cells, the PC synthesis was enhanced under Cd exposure (Fig.…”

Section: Discussionmentioning

confidence: 96%

“…Hence, another mechanism of Cd detoxification is present in the CCdl cells. According to some reports, Cd induces the activity of a catalase (Stroinski and Koz10wska 1997;Stroinski and Zielezinska 1997) and guaiacol and ascorbate peroxidases (Reddy and Prasad 1992;Chaoui et al 1997) in plants. We have recently observed that the antioxidative defense system is also enhanced by Cd in carrot cells.…”

Section: Discussionmentioning

confidence: 99%

Characterization of cadmium-tolerant carrot cells in response to cadmium stress

Kim

Takeuchi

Hara

et al. 2000

Soil Science and Plant Nutrition

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“…PCs were isolated and separated according to Stroiński & Zielezińska (1997), using the method presented by Tukendorf & Rauser (1990). Frozen leaves (0.5 g) were homogenized with an Art-Miccra homogenizer in 2 ml of 0.5 mmol dm -3 HCl.…”

Section: Thiols Content and Phytochelatin Synthase Activitymentioning

confidence: 99%

Induction of phytochelatins and flavonoids in cadmium polluted Berberis thunbergii

Chadzinikolau¹,

Kozłowska²,

Mleczek³

2017

Dendrobiology

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Cadmium tolerance has been widely studied in various plant species. The study was undertaken to assess the response of Berberis thunbergii, commonly growing under urban conditions, to increasing concentrations of cadmium (1, 4 and 16 mg of Cd kg -1 soil). Experiments were conducted from June to August in a greenhouse. Accumulation of cadmium ions in barberry leaves was observed, at a simultaneous occurrence of mechanisms reducing its harmful effects by an increased synthesis of phytochelatins (PCs). Generation of PCs -putative heavy metal chelators was related to the phytochelatin synthase activity (PCS) and the level of metal accumulation. Increased contents of anthocyanins and flavonols in cadmium polluted barberry, considering that it is a coloured-leaf plant seems important in aspect of their multifunctional role in plant-environment interactions. Besides the capacity to chelate heavy metals, flavonoids are antioxidants and efficiently scavenge free radicals. Moreover, the content of chloroplast pigments was determined, but the changes were non-significant.

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Cadmium effect on hydrogen peroxide, gluthatione and phytochelatins levels in potato tuber

Cited by 24 publications

References 38 publications

Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens

Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens

Characterization of cadmium-tolerant carrot cells in response to cadmium stress

Induction of phytochelatins and flavonoids in cadmium polluted Berberis thunbergii

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