(CAC)<sub>5</sub>, a very informative oligonucleotide probe for DNA fingerprinting

Schäfer, Renate; Zischler, Hans; Epplen, Jörg T.

doi:10.1093/nar/16.11.5196

Cited by 70 publications

(25 citation statements)

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“…Epplen's group showed that the oligonucleotide (CAC), is a highly informative probe for D N A fingerprinting [ 15, 161. Even though (CAC), has been mostly discussed in the context of GATA/GACA simple quadruplet repeats [ 15,16,351 it is relevant to point out that it hybridizes to GTGGTGGTGGTGGTG, a sequence which is clearly homologous to the other CHI-related fingerprinting probes already discussed above. This demonstration that oligonucleotide probes can be used for D N A fingerprinting had great appeal to us for two reasons.…”

Section: The (Cac) Oligonucleotidementioning

confidence: 99%

DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probes

et al. 1991

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The discovery of multilocal DNA fingerprinting represented a revolution in criminal identification and paternity testing. However, for routine use in clinical laboratories, the standard DNA fingerprint methodology is too complex. We have been successful in the development of a simplified DNA nonisotopic fingerprinting system using biotin-labeled probes which we have called DNA bioprints. To achieve this we explored three main technical features: utilization of biotinylated nonradioactive probes as a simpler substitute for 32P-labeled probes, utilization of oligonucleotide probes as a simpler substitute for recombinant probes, and direct hybridization in the dried agarose gel as a simpler substitute for Southern blots. In this article we review our results in the development of DNA bioprints.

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Section: The (Cac) Oligonucleotidementioning

confidence: 99%

DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probes

et al. 1991

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“…The technique targets a large number of hypervariable loci that are considered to be distributed over the entire genome. In DNA fingerprinting, simple sequence repeats, such as (CAC)s (Schafer et al, 1988), and minisatellite sequences, such as Jeffreys' 33.6 and 33.15 (Jeffreys et al, 1985), have been used as probes that hybridize to a large number of restriction fragments of genomic DNA. Because allelic variation in the repeat unit number and mutation in genomic DNA alter the profiles of DNA restriction fragments, the band patterns of DNA fingerprinting display a high degree of polymorphism (Jeffreys, 1987).…”

Section: Introductionmentioning

confidence: 99%

Measurement of Genetic Parameters Within and Between Turkey Lines Using DNA Fingerprinting

et al. 1996

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An experiment was conducted to estimate genetic parameters in six experimental and five commercial primary breeding turkey lines using DNA fingerprinting. Eighteen individual DNA samples per line were digested with an HaeIII restriction enzyme and hybridized with Jeffreys' 33.6 probe. The DNA fingerprints were analyzed with computer programs to measure band sharing (BS) and band frequencies. Within lines, BS ranged from 0.39 to 0.62 and reflected the history of the experimental lines. Among lines, BS ranged from 0.21 to 0.33 with an average of 0.26. The BS among the experimental lines reflected known relationships. All lines were subdivided based on indices of population subdivision. About 26 hypervariable loci were estimated from band frequencies. Average heterozygosity and genetic variability estimated from band frequencies were significantly different among lines and displayed a result very similar to the BS among lines. Genetic distance indices among lines were also significantly different and reflected known relationships between the experimental lines. The experimental selected lines displayed lower genetic diversity than did the other lines. The parameters measuring genetic diversity within lines had higher correlation coefficients among them than did the parameters between lines. The computer program used in this study made DNA fingerprinting easier to use in population analysis.

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“…The pedigree shown almost certainly underestimates the level of inbreeding that has occurred in this family, since detailed records were only available on males in the family; moreover, inbreeding had probably occurred for many generations before the pedigree begins. Four multi-locus probes, 33.6 and 33.15 (Jeffreys et ah 1985a), (CAC)s (Schafer et al 1988) and M13 (Vassart et ah 1987) were used to produce D N A fingerprints of all or part of the Gaza family. British families were used as outbred controls.…”

Section: Introductionmentioning

confidence: 99%

Increased band sharing in DNA fingerprints of an inbred human population

et al. 1991

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We have compared band sharing between the DNA fingerprints of members of an inbred human population with band sharing between members of an outbred population. It had not previously been determined whether the high rate of mutation at minisatellite loci is sufficient to prevent an increase in band sharing in moderately inbred populations. We have found that there is an increase in band sharing in the 2-kb to 9-kb size range, but not in the greater than 9-kb size range, in the inbred population. The difference was consistently observed using four different multi-locus probes, viz. 33.6, 33.15, (CAC)5 and M13. Thus, we have demonstrated that moderate but prolonged inbreeding can lead to increased similarity in human DNA fingerprints. This should be considered when analysing DNA fingerprints in forensic or paternity cases involving members of an inbred community.

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(CAC)₅, a very informative oligonucleotide probe for DNA fingerprinting

Cited by 70 publications

References 2 publications

DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probes

DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probes

Measurement of Genetic Parameters Within and Between Turkey Lines Using DNA Fingerprinting

Increased band sharing in DNA fingerprints of an inbred human population

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(CAC)5, a very informative oligonucleotide probe for DNA fingerprinting

Cited by 70 publications

References 2 publications

DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probes

DNA bioprints: Simple nonisotopic DNA fingerprints with biotinylated probes

Measurement of Genetic Parameters Within and Between Turkey Lines Using DNA Fingerprinting

Increased band sharing in DNA fingerprints of an inbred human population

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Resources

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(CAC)₅, a very informative oligonucleotide probe for DNA fingerprinting