Ca2+ response of rat mesangial cells to ATP analogues

Gutiérrez, Antonio; Lou, Xiaofen; Erik, A.; Persson, Gunnar; Ring, Avi

doi:10.1016/s0014-2999(99)00032-1

Cited by 25 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, it seems that ATP-induced Ca 2ϩ responses were induced by activation of P2Y 2 -purinergic receptors. This finding is in good agreement with previous studies (25)(26)(27). The concentration-response curve (Fig.…”

Section: Resultssupporting

confidence: 93%

Macula densa cell signaling involves ATP release through a maxi anion channel

Bell

Lapointe

Sabirov

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

256

282

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 93%

Macula densa cell signaling involves ATP release through a maxi anion channel

Bell

Lapointe

Sabirov

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

256

282

View full text Add to dashboard Cite

show abstract

“…In their studies (20,33), it is possible that the A1 receptor agonist directly affected the arteriolar smooth muscle cells. As recently reported (23,34), freshly dissected renal arterioles but not cultured mesangial cells respond to adenosine with an increase in [Ca 2ϩ ] i . Alternatively, it is possible that macula densa cells do possess adenosine receptors but that these receptors are not wired to the intracellular Ca 2ϩ messenger system.…”

Section: Discussionsupporting

confidence: 61%

“…The order of the efficacy of the nucleotides in macula densa cells was essentially the same as that found for P2Y 2 and/or P2Y 4 receptors (23,(41)(42)(43)(44); however, this assertion should be viewed with caution. It should be kept in mind that there are complicating factors inherent in functional methods used in establishing purinergic receptor subtypes (45).…”

Section: Discussionsupporting

confidence: 53%

Purinergic Receptor Signaling at the Basolateral Membrane of Macula Densa Cells

Liu¹,

Bell²,

Peti‐Peterdi³

et al. 2002

Journal of the American Society of Nephrology

View full text Add to dashboard Cite

Abstract. Purinergic receptors are important in the regulation of renal hemodynamics; therefore, this study sought to determine if such receptors influence macula densa cell function. Isolated glomeruli containing macula densa cells, with and without the cortical thick ascending limb, were loaded with the Ca 2ϩ sensitive indicators, Fura Red (confocal microscopy) or fura 2 (conventional video image analysis). Studies were performed on an inverted microscope in a chamber with a flowthrough perfusion system. Changes in cytosolic calcium concentration ([Ca 2ϩ ] i ) from exposed macula densa plaques were assessed upon addition of adenosine, ATP, UTP, ADP, or 2-methylthio-ATP (2-MeS-ATP) for 2 min added to the bathing solution. The tubuloglomerular feedback (TGF) mechanism is a very important regulator of renal hemodynamics. This mechanism operates as a negative feedback loop, sensing changes in distal nephron fluid flow rate by detecting flow-dependent alterations in luminal sodium chloride concentration ([NaCl]) at the macula densa. Signals are then sent by the macula densa cells, which contract the afferent arteriole, thereby adjusting the level of GFR and renal blood flow (1-3). Furthermore, macula densa cells can also influence release of renin from the granular cells in the afferent arteriole (4). The primary mechanism by which the macula densa cells detect changes in [NaCl] is through an apically located Na:2Cl:K cotransport mechanism (5,6). It has also been found that the sensitivity of this TGF mechanism can be reset by a large number of different factors. For instance, renal interstitial pressure and angiotensin II are potent regulators of TGF sensitivity (7,8). Nitric oxide, prostaglandin, thromboxanes, and many other local factors and hormones can also influence the sensitivity of the TGF mechanism (9). The site(s) at which all these factors exert their influence is not well understood. One possibility is that modulation of TGF can occur at the level of the macula densa cells, and it is therefore of particular interest to investigate the receptors expressed by macula densa cells and what effects occur in these cells with receptor activation. For instance, it has recently been reported that macula densa cells possess AT 1 receptors for angiotensin II and that activation of these receptors increases sodium-proton exchange of the apical NHE2 isoform (10 -12).Purinergic receptors have been identified as playing a large role within the juxtaglomerular apparatus (13). These receptors are activated by extracellular purines (adenosine, ADP, and ATP) and pyrimidines (UDP and UTP) and are important signaling molecules that mediate various biologic effects in the kidney. They may serve as paracrine regulators of renal microvascular resistance (14,15) and may modulate mesangial cell contraction, alter epithelial ion transport, and influence the TGF mechanism (16 -20) via cell surface receptors for purines.There are two main families of purine receptors: adenosine or P1 receptors and P2 receptors, the latter recogni...

show abstract

“…15 The mitogenic effects via P2Y receptors are linked to stimulation of phospholipase C and Ca 2ϩ release from inositol-phosphate-sensitive intracellular stores, and they are synergistic with those induced by conventional polypeptide growth factors. 16 The signal transduction includes nucleotide-stimulated inositol trisphosphate and 1,2-diacylglycerol formation 17,18 and the subsequent Ca 2ϩ mobilization, 19,20 protein kinase C activation, 21 and prostaglandin E 2 synthesis, 17 the stimulation of phospholipase D, 22 and the activation of the P42/P44 mitogen-activated protein kinase (MAPK) pathway and subsequent cell proliferation. [23][24][25] Furthermore, ERKs can be activated via P2Y receptors and the upstream activator MEKs such as MEK1-3; the intracellular signaling cascades vary between receptor subtypes.…”

Section: Discussionmentioning

confidence: 99%

Increased Uridine Adenosine Tetraphosphate Concentrations in Plasma of Juvenile Hypertensives

Jankowski

Meyer

Schlattmann

et al. 2007

ATVB

View full text Add to dashboard Cite

Background-Uridine adenosine tetraphosphate (Up 4 A) was been recently characterized as a potent vasoconstrictor. Up 4 A occurs in plasma from healthy subjects at concentrations sufficient to cause strong vasoconstrictive effects. In this study, Up 4 A concentrations in plasma from juvenile hypertensives and normotensives were determined. Methods and Results-Up 4 A was purified to homogeneity by preparative reverse phase high performance liquid-chromatography (HPLC), affinity chromatography HPLC, and analytic reverse phase HPLC from deproteinized plasma of juvenile hypertensives and normotensives.

show abstract

Ca2+ response of rat mesangial cells to ATP analogues

Cited by 25 publications

References 22 publications

Macula densa cell signaling involves ATP release through a maxi anion channel

Macula densa cell signaling involves ATP release through a maxi anion channel

Purinergic Receptor Signaling at the Basolateral Membrane of Macula Densa Cells

Increased Uridine Adenosine Tetraphosphate Concentrations in Plasma of Juvenile Hypertensives

Contact Info

Product

Resources

About