Ca2+-Induced Mitochondrial ROS Regulate the Early Embryonic Cell Cycle

Han, Yue; Ishibashi, Shoko; Iglesias-Gonzalez, Javier; Chen, Yaoyao; Love, Nick R.; Amaya, Enrique

doi:10.1016/j.celrep.2017.12.042

Cited by 86 publications

(93 citation statements)

References 60 publications

(69 reference statements)

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“…Using a previously established transgenic line, HyPer (Love et al, 2013;, which allows the assessment of ROS levels in vivo, we recently showed that fertilisation triggers a burst of ROS production in Xenopus, which plays a critical role in the regulation of the early embryonic cell cycle (Han et al, 2017). Here we extend that work by showing that the fertilisation induced rise in ROS levels are sustained throughout early embryonic development.…”

Section: Introductionsupporting

confidence: 61%

“…Transgenic F1 Xenopus laevis females expressing HyPer maternally were used (Love et al, 2011). Imaging was performed as previously described (Han et al, 2017).…”

Section: Imaging and Detection Of H 2 Omentioning

confidence: 99%

“…We have established a method for visualizing H 2 O 2 in Xenopus oocytes and early embryos in vivo by employing a transgenic reporter line, which expresses a genetic sensor H 2 O 2 , named Hyper, maternally (Han et al, 2017;Love et al, 2013;.…”

Section: An Oxidative Burst Of H 2 O 2 Induced At Fertilisation Is Sumentioning

confidence: 99%

“…Using this resource, we have previously shown that the H 2 O 2 levels increased following fertilisation (Han et al, 2017). We next sought to determine whether the fertilisationinduced H 2 O 2 production was transient or sustained throughout development.…”

Section: An Oxidative Burst Of H 2 O 2 Induced At Fertilisation Is Sumentioning

confidence: 99%

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Elevated and sustained reactive oxygen species levels facilitate mesoderm formation during earlyXenopusdevelopment

Han

Chen

et al. 2017

Preprint

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Fertilisation triggers embryonic development culminating with the activation of a number of highly co-ordinated and evolutionarily conserved signalling pathways, which induce and pattern the mesoderm of the developing embryo. Previous studies in invertebrates have shown that hydrogen peroxide (H 2 O 2 ), a reactive oxygen species (ROS), can act as a signalling molecule for axis specification during early development.Using a HyPer transgenic Xenopus laevis line that expresses a H 2 O 2 -sensitive fluorescent protein sensor maternally, we recently found that fertilisation triggers a rapid increase in ROS production. Here we show that this increase in ROS levels is sustained throughout early embryogenesis, lasting until the tailbud stages. In addition we show that lowering ROS levels from the blastula stage through the gastrula stages via antioxidant treatments disrupts mesoderm formation. Furthermore, we show that attenuating ROS levels during the blastula / gastrula stages affects some, but not all, growth factor signalling pathways involved in mesoderm induction and patterning, including the PI3K/Akt, TGF-β/Nodal, and Wnt/β-catenin signalling pathways. These data suggest that sustained elevated ROS levels during the blastula and gastrula stages are essential for early vertebrate embryonic development, at least partly, through their roles in promoting growth factor signalling.

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Section: Introductionsupporting

confidence: 61%

“…Transgenic F1 Xenopus laevis females expressing HyPer maternally were used (Love et al, 2011). Imaging was performed as previously described (Han et al, 2017).…”

Section: Imaging and Detection Of H 2 Omentioning

confidence: 99%

Section: An Oxidative Burst Of H 2 O 2 Induced At Fertilisation Is Sumentioning

confidence: 99%

Section: An Oxidative Burst Of H 2 O 2 Induced At Fertilisation Is Sumentioning

confidence: 99%

See 2 more Smart Citations

Elevated and sustained reactive oxygen species levels facilitate mesoderm formation during earlyXenopusdevelopment

Han

Chen

et al. 2017

Preprint

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“…These gap junctions could allow for diffusion of small molecules, such as calcium, which could in turn affect the cell cycle. For example, it has been recently suggested that calcium‐induced mitochondrial reactive‐oxygen species could control the cell cycle by regulating Cdc25C activity . Future experiments blocking gap junctions might reveal important insights on the mechanisms of mitotic waves.…”

Section: The Embryonic Cell Cycle: a Bistable Switch Within A Non‐linmentioning

confidence: 99%

Chemical Waves in Embryonic Cell Cycles

Ferree

Talia

2018

Israel Journal of Chemistry

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In most metazoans, early embryonic development is characterized by a rapid series of cleavage divisions. At the core of the coordination of these divisions is the oscillatory dynamic of Cyclin‐dependent kinase 1 (Cdk1), which arises through the integration of a negative feedback with delay and positive feedback. The regulation of this oscillator in large embryos is emerging as an ideal model for quantitative studies of how spatiotemporal coordination is achieved in large complex tissues. Recent work has demonstrated that the reaction‐diffusion dynamics regulating the cell cycle can generate traveling waves of enzymatic activity, which ensure the synchronization of cell division processes. Here, we will review how the mechanisms of cell cycle regulation can give rise to chemical waves, and highlight recent experiments on the coordination of cell division through traveling waves.

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The critical role of cardiolipin in metazoan differentiation, development, and maturation

Olivar-Villanueva

Ren

Schlame

et al. 2023

Developmental Dynamics

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Cardiolipins are phospholipids that are central to proper mitochondrial functioning. Because mitochondria play crucial roles in differentiation, development, and maturation, we would also expect cardiolipin to play major roles in these processes. Indeed, cardiolipin has been implicated in the mechanism of three human diseases that affect young infants, implying developmental abnormalities. In this review, we will: (1) Review the biology of cardiolipin; (2) Outline the evidence for essential roles of cardiolipin during organismal development, including embryogenesis and cell maturation in vertebrate organisms; (3) Place the role(s) of cardiolipin during embryogenesis within the larger context of the roles of mitochondria in development; and (4) Suggest avenues for future research.

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Ca2+-Induced Mitochondrial ROS Regulate the Early Embryonic Cell Cycle

Cited by 86 publications

References 60 publications

Elevated and sustained reactive oxygen species levels facilitate mesoderm formation during earlyXenopusdevelopment

Elevated and sustained reactive oxygen species levels facilitate mesoderm formation during earlyXenopusdevelopment

Chemical Waves in Embryonic Cell Cycles

The critical role of cardiolipin in metazoan differentiation, development, and maturation

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