Ca2+-dependent successive phosphorylation of vacuolar transporter MTP8 by CBL2/3-CIPK3/9/26 and CPK5 is critical for manganese homeostasis in Arabidopsis

Ju, Chuanfeng; Zhang, Zhenqian; Deng, Jinping; Miao, Cuicui; Wang, Zhangqing; Wallrad, Lukas; Javed, Laiba; Fu, Dali; Zhang, Ting; Kudla, Jörg; Gong, Zhizhong; Wang, Cun

doi:10.1016/j.molp.2021.11.012

Cited by 36 publications

(36 citation statements)

References 78 publications

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“…A typical CBL-CIPK signaling module is activated by elevation in free cytosolic Ca 2+ that binds to CBLs through their EF hand motifs and trigger conformational changes. Activated CBLs interact with CIPKs that transphosphorylate and modulate the activity of downstream targets such as ion transporters 16,[27][28][29][30][31][32][33][34] . In the case of low-K + response, K + deficiency has been shown to triggers distinct Ca 2+ changes in Arabidopsis roots 35 , which may activate CBL-CIPK signaling pathways that in turn promote K + -uptake and/or vacuolar remobilization.…”

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confidence: 99%

Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis

Tang

Wang

et al. 2023

Nat Commun

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Under low-potassium (K+) stress, a Ca2+ signaling network consisting of calcineurin B-like proteins (CBLs) and CBL-interacting kinases (CIPKs) play essential roles. Specifically, the plasma membrane CBL1/9-CIPK pathway and the tonoplast CBL2/3-CIPK pathway promotes K+ uptake and remobilization, respectively, by activating a series of K+ channels. While the dual CBL-CIPK pathways enable plants to cope with low-K+ stress, little is known about the early events that link external K+ levels to the CBL-CIPK proteins. Here we show that K+ status regulates the protein abundance and phosphorylation of the CBL-CIPK-channel modules. Further analysis revealed low K+-induced activation of VM-CBL2/3 happened earlier and was required for full activation of PM-CBL1/9 pathway. Moreover, we identified CIPK9/23 kinases to be responsible for phosphorylation of CBL1/9/2/3 in plant response to low-K+ stress and the HAB1/ABI1/ABI2/PP2CA phosphatases to be responsible for CBL2/3-CIPK9 dephosphorylation upon K+-repletion. Further genetic analysis showed that HAB1/ABI1/ABI2/PP2CA phosphatases are negative regulators for plant growth under low-K+, countering the CBL-CIPK network in plant response and adaptation to low-K+ stress.

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confidence: 99%

Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis

Tang

Wang

et al. 2023

Nat Commun

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“…A semi‐ vivo protein kinase assay was conducted according to a previously published method (Ju et al ., 2022). The 7‐d‐old CPK21 OE and CPK23 OE transgenic seedlings growing in ½MS medium were transferred to 80 μM CdCl 2 stress medium (½MS + 80 μM CdCl 2 ) for the indicated times (0, 3, 6, 24, and 36 h), and the total proteins were extracted and purified for semi‐ vivo phosphorylation assays.…”

Section: Methodsmentioning

confidence: 99%

“…The bimolecular fluorescence complementation (BiFC) assay was conducted according to a previously published method (Ju et al ., 2022). CPKs‐nYFP and NRAMP6‐cYFP constructs were transiently expressed in N. benthamiana leaves.…”

Section: Methodsmentioning

confidence: 99%

Plasma membrane‐associated calcium signaling modulates cadmium transport

et al. 2023

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Summary Cadmium (Cd) is a toxic heavy element for plant growth and development, and plants have evolved many strategies to cope with Cd stress. However, the mechanisms how plants sense Cd stress and regulate the function of transporters remain very rudimentary. Here, we found that Cd stress induces obvious Ca2+ signals in Arabidopsis roots. Furthermore, we identified the calcium‐dependent protein kinases CPK21 and CPK23 that interacted with the Cd transporter NRAMP6 through a variety of protein interaction techniques. Then, we confirmed that the cpk21 23 double mutants significantly enhanced the sensitive phenotype of cpk23 single mutant under Cd stress, while the overexpression and continuous activation of CPK21 and CPK23 enhanced plants tolerance to Cd stress. Multiple biochemical and physiological analyses in yeast and plants demonstrated that CPK21/23 phosphorylate NRAMP6 primarily at Ser489 and Thr505 to inhibit the Cd transport activity of NRAMP6, thereby improving the Cd tolerance of plants. Taken together, we found a plasma membrane‐associated calcium signaling that modulates Cd tolerance. These results provide new insights into the molecular breeding of crop tolerance to Cd stress.

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“…Instead, we will need to gain a non‐preceded understanding of the temporal and spatial resolution in which these processes proceed in certain cells and cell types of the plant. The most recent discovery of a temporally two‐tiered but functionally antagonistic regulation of the vacuolar Mn transporter MTP8 by CPKs and CIPKs, respectively, may provide a guiding example in this direction (Ju et al, 2021). In this work, it was reported that sequential phosphorylation of MTP8 initially at Ser31/32 by the calcium‐dependent protein kinases CPK5 and subsequently at Ser35 by CIPK26 provides an activation/deactivation fine‐tuning mechanism for differential Mn transport regulation.…”

Section: Getting the Message: Components And Mechanisms Decoding Stre...mentioning

confidence: 99%

Ca²⁺ signaling in plant responses to abiotic stresses

Dong¹,

Wallrad²,

Almutairi

et al. 2022

JIPB

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Adverse variations of abiotic environmental cues that deviate from an optimal range impose stresses to plants. Abiotic stresses severely impede plant physiology and development. Consequently, such stresses dramatically reduce crop yield and negatively impact on ecosystem stability and composition. Physical components of abiotic stresses can be, for example, suboptimal temperature and osmotic perturbations, while representative chemical facets of abiotic stresses can be toxic ions or suboptimal nutrient availability. The sheer complexity of abiotic stresses causes a multitude of diverse components and mechanisms for their sensing and signal transduction. Ca 2+ , as a versatile second messenger, plays multifaceted roles in almost all abiotic stress responses in that, for a certain abiotic stress, Ca 2+ is not only reciprocally connected with its perception, but also multifunctionally ensures subsequent signal transduction. Here, we will focus on salt/osmotic stress and responses to altered nutrient availability as model cases to detail novel insights into the identity of components that link stress perception to Ca 2+ signal formation as well as on new insights into mechanisms of Ca 2+ signal implementation. Finally, we will deduce emerging conceptual consequences of these novel insights and outline arising avenues of future research on the role of Ca 2+ signaling in abiotic stress responses in plants.

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Ca2+-dependent successive phosphorylation of vacuolar transporter MTP8 by CBL2/3-CIPK3/9/26 and CPK5 is critical for manganese homeostasis in Arabidopsis

Cited by 36 publications

References 78 publications

Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis

Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis

Plasma membrane‐associated calcium signaling modulates cadmium transport

Ca²⁺ signaling in plant responses to abiotic stresses

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Ca2+-dependent successive phosphorylation of vacuolar transporter MTP8 by CBL2/3-CIPK3/9/26 and CPK5 is critical for manganese homeostasis in Arabidopsis

Cited by 36 publications

References 78 publications

Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis

Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis

Plasma membrane‐associated calcium signaling modulates cadmium transport

Ca2+ signaling in plant responses to abiotic stresses

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Ca²⁺ signaling in plant responses to abiotic stresses