C60 fullerene affects elastic properties and osmoregulation reactions of human lymphocytes

Abstract

The influence of aqueous solution of pristine C60 fullerene (C60FAS) on functional activity of lymphocytes from a healthy person was studied for the first time. By means of atomic force microscopy, it was found that C60FAS in a concentration of 0.1 mg/ml increases the stiffness of the lymphocyte membrane by 41% (p < 0.05) and lowers the functional activity of the plasmalemma surface, thereby constraining the use of its membrane material in physiological reactions using a hypotonic model in vitro. However, a cell retains the ability to regulate its volume and demonstrates relative resistance to hypo-osmotic stress. The resistance of lymphocytes in hypo-osmotic medium is facilitated by activation of the nucleus by C60 fullerene particles, which regulates the implementation of two consistent phases of an increase and decrease of cell volume, thereby retaining cell viability. All these indicate the impact of C60 fullerene on the cellular nucleus.

Comparative Analysis of the Antineoplastic Activity of C60 Fullerene with 5-Fluorouracil and Pyrrole Derivative In Vivo

“…The absence of specific peculiarities on the curve suggests that the particles are polydisperse in size. It is in a good agreement with probe microscopy data [40, 41]. At sufficiently small q values ( qR g  < 1), the scattered intensity can be described by the Guinier approximation

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Gene Delivery with Organic Electronic Biomaterials

“…Unmodified bucky balls are hydrophobic rendering them poorly soluble in aqueous media, and interesting fundamental studies show that they interact with amphiphilic cell membranes prior to uptake [22][23][24][25], they are relatively quickly cleared from blood (accumulating in the liver and spleen [25]), and they exhibit dose-dependent toxicity in vivo (low toxicity at low concentrations in mice, and high toxicity at high concentrations with potential for mutagenic/teratogenic activity) [25].…”

Effect of C60 nanoparticles on elasticity of small unilamellar vesicles composed of DPPC bilayers*

“…[30] Meanwhile, Skorkina et al found that the aqueous solution with the C 60 concentration of 0.1 mg/ml can increase the stiffness of the lymphocyte membrane by 41%. [31] In addition, Zhou et al revealed that the incorporation of C 60 with a molar concentration of 0.8% can increase Young's modulus of gel phase lipid bilayers, but barely changes that of fluid phase bilayers. [32] On the other hand, molecular dynamics (MD) simulations showed that C 60 molecules are capable of translocating to the internal region of lipid bilayers spontaneously, affecting the structures and mechanic properties of plane membranes as well as inducing the dynamic interactions.…”