2016
DOI: 10.1016/j.mad.2016.01.004
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Abstract: Moderate stress can increase lifespan by hormesis, a beneficial low-level induction of stress response pathways. 5’-fluorodeoxyuridine (FUdR) is commonly used to sterilize Caenorhabditis elegans in aging experiments. However, FUdR alters lifespan in some genotypes and induces resistance to thermal and proteotoxic stress. We report that hypertonic stress in combination with FUdR treatment or inhibition of the FUdR target thymidylate synthase, TYMS-1, extends C. elegans lifespan by up to 30%. By contrast, in the… Show more

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Cited by 64 publications
(56 citation statements)
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“…Curiously, although Drosophila Dicer proteins cleave these VSV snapback particles, the resulting siRNAs are, for unknown reasons, not loaded into Argonautes [39]. It has been postulated that these interfering particles may serve as a RNA decoy, allowing VSV evasion of RNAi machinery [39, 40]. It will be interesting to determine if VSV produces defective interfering particles in C. elegans and whether small RNAs targeting these particles are loaded into RDE-1.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Curiously, although Drosophila Dicer proteins cleave these VSV snapback particles, the resulting siRNAs are, for unknown reasons, not loaded into Argonautes [39]. It has been postulated that these interfering particles may serve as a RNA decoy, allowing VSV evasion of RNAi machinery [39, 40]. It will be interesting to determine if VSV produces defective interfering particles in C. elegans and whether small RNAs targeting these particles are loaded into RDE-1.…”
Section: Discussionmentioning
confidence: 99%
“…How this DNA synthesis inhibitor might compromise germline immunity to infection is unknown. However, FUdR treatment can modulate resistance to thermal, hypertonic, and proteotoxic stresses, likely through alteration of gene expression [40]. Therefore, FUdR-induced inhibition of germline gene expression programs and/or stress responses that normally serve antiviral roles may promote germline infection and vertical transmission.…”
Section: Discussionmentioning
confidence: 99%
“…Beyond this specific developmental stage, C22 can be used in studies such as aging assays, where it could serve as an alternative to FUDR treatment to block reproduction (Gandhi et al, 1980;Mitchell et al, 1979). FUDR can be problematic as it has been shown to artificially enhance lifespan in several mutant backgrounds (Aitlhadj and Stürzenbaum, 2010;Anderson et al, 2016;Davies et al, 2012;Van Raamsdonk and Hekimi, 2011). C22 has the added advantage of not altering rates of germ cell proliferation, which can also affect lifespan.…”
Section: Discussionmentioning
confidence: 99%
“…spe-9(hc88) strains are widely used as an alternative to 5-fluoro-2′-deoxyuridine (FUDR) sterilization, and have been validated to have wild-type lifespans at the restrictive temperature of 25.5°C (Fabian and Johnson, 1994) and wild-type brood sizes at the permissive temperatures of 16°C and 20 °C (Singson et al, 1998). We used spe-9(hc88) to avoid the known confounding effects of FUDR (Anderson et al, 2016) and to eliminate issues due to the timing of administration (such as being administered at different phases of life for fast- and slow-developing individuals on the same slide).…”
Section: Methods and Resourcesmentioning
confidence: 99%