bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm.

Carbonero, Pilar; Vicente‐Carbajosa, Jesús; Mena, Montaña; Oñate, Luis; Lara, Pilar; Dı́az, Isabel; Black, Morgan; Bradford, Kent J.; Vázquez‐Ramos, Jorge M.

doi:10.1079/9780851994048.0027

Cited by 4 publications

(7 citation statements)

References 17 publications

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“…Ultimately, over expression and knock out of SPA could be of interest in studying the influence of this gene on storage protein synthesis. Moreover, it should be remembered that other transcriptional factors (Carbonero et al 2000;Diaz et al 2002) are involved in the complex regulatory pathway of cereal storage proteins, the dissection of which has just begun.…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptional factors binding to specific DNA motifs are involved in such a regulation, as shown in the cases of Opaque-2 (Motto et al 1989) and prolamin box binding factor (PBF) (Vicente-Carbajosa et al 1997). In barley, four transcription factors involved in the expression of genes coding for hordein seed storage proteins have been identified (Mena et al 1998;Vicente-Carbajosa et al 1998;Oñate et al 1999;Carbonero et al 2000;Diaz et al 2002). In wheat, Hammond-Kosack et al (1993) demonstrated that two protein factors, endosperm box factors I and II (ESBF-I and ESBF-II), could bind to the bifactorial cis-acting element of an LMW-GS gene (Colot et al 1989).…”

Section: Introductionmentioning

confidence: 97%

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Colocation between a gene encoding the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat (Triticum aestivum)

Guillaumie¹,

Charmet²,

Linossier³

et al. 2004

Genome

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The quality of wheat grain is largely determined by the quantity and composition of storage proteins (prolamins) and depends on mechanisms underlying the regulation of expression of prolamin genes. The endosperm-specific wheat basic region leucine zipper (bZIP) factor storage protein activator (SPA) is a positive regulator that binds to the promoter of a prolamin gene. The aim of this study was to map SPA (the gene encoding bZIP factor SPA) and genomic regions associated with quantitative variations of storage protein fractions using F7 recombinant inbred lines (RILs) derived from a cross between Triticum aestivum "Renan" and T. aestivum "Récital". SPA was mapped through RFLP using a cDNA probe and a specific single nucleotide polymorphism (SNP) marker. Storage protein fractions in the parents and RILs were quantified using capillary electrophoresis. Quantitative trait loci (QTLs) for protein were detected and mapped on six chromosome regions. One QTL, located on the long arm of chromosome 1B, explained 70% of the variation in quantity of the x subunit of Glu-B1. Genetic mapping suggested that SPA is located on chromosome arm 1L and is also present in the confidence interval of the corresponding QTL for Glu-B1x on 1BL, suggesting that SPA might be a candidate gene for this QTL.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Colocation between a gene encoding the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat (Triticum aestivum)

Guillaumie¹,

Charmet²,

Linossier³

et al. 2004

Genome

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“…The pattern of expression of cereal seed genes during endosperm development is highly regulated in a spatial and temporal manner and much information is available concerning cis motifs in their promoters and the transcription factors (TFs) that interact with them. A survey of protein storage genes ( Hor2 ) and other seed‐specific genes, putatively involved in defence, such as that encoding trypsin inhibitor CMe (gene Itr1 ), shows that several consensus sequences are highly conserved in their promoter regions (Carbonero et al. , 2000; Diaz et al.…”

Section: Introductionmentioning

confidence: 99%

HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination

Rubio‐Somoza¹,

Martínez²,

Dı́az³

et al. 2005

The Plant Journal

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SummaryThe functional analysis of hydrolase gene promoters induced by gibberellin (GA) in barley aleurone cells upon germination has identified a tripartite GA-response complex (GARC) containing a 5¢-TATCCAC-3¢ box as well as the GA-responsive element (GARE) recognized by GAMYB and the pyrimidine box interacting with the DOF transcription factors BPBF and SAD. We show here that the MCB1 gene encoding a R1MYB protein binds to the 5¢-TATCCAC-3¢ (GATA core) box in vitro and is a transcriptional repressor of a GA-induced amylase (Amy6.4) promoter in bombarded aleurone layers. Northern blot and mRNA in situ hybridization analyses showed that the MCB1 transcripts accumulate in the aleurone cells upon germination, as well as in endosperm tissues during seed development. The HvMCB1 protein expressed in bacteria binds in a specific manner to a 27-mer oligonucleotide containing the 5¢-TATCCAC-3¢ sequence, derived from the promoter region of the Amy6.4 gene. Accumulation of the MCB1 transcript diminished in response to external GA incubation in aleurone cells, and in transient expression experiments HvMCB1 repressed transcription of the Amy6.4 promoter in GAtreated aleurone layers and reversed the GAMYB-mediated activation of this amylase promoter. In contrast, during endosperm maturation HvMCB1 acted as a transcription activator of the seed-specific Itr1 gene promoter through binding to a 5¢-GATAAGATA-3¢ box.

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“…A third motif, 5′‐AACAAC‐3′, has been shown to play a role in the expression of genes encoding glutelins (globulin‐like proteins) in rice of the grass subfamily Bambusoideae. These three types of motif are the target sites of different transcription factor (TF) classes: (i) the GLM is recognized by bZIP proteins of the Opaque2 family (Albani et al ., 1997; Muth et al ., 1996; Oñate et al ., 1999; Schmidt et al ., 1992; Vicente‐Carbajosa et al ., 1998; Wu et al ., 1998); (ii) the PB is bound by TFs of the DOF (DNA‐binding with one finger) class, generically designated as prolamine‐box binding factors (PBF; Carbonero et al. , 2000; Mena et al ., 1998; Vicente‐Carbajosa et al ., 1997; Yanagisawa, 1996); (iii) the 5′‐AACAAC‐3′ motif in rice glutelin gene promoters is recognized by a TF of the MYB class, OsMYB5 (Suzuki et al ., 1998).…”

Section: Introductionmentioning

confidence: 99%

The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm‐specific genes during seed development

Dı́az¹,

Vicente‐Carbajosa²,

Abraham³

et al. 2002

The Plant Journal

220

213

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SummaryHvGAMYB, a MYB transcription factor previously shown to be expressed in barley aleurone cells in response to gibberellin during germination, also has an important role in gene regulation during endosperm development. The mRNA was detected early (10 DAF) in the seeds where it accumulates, not only in the aleurone layer, starchy endosperm, nucellar projection and vascular tissue, but also in the immature embryo as shown by in situ hybridization analysis. The HvGAMYB protein, expressed in bacteria, binds to oligonucleotides containing the 5¢-TAACAAC-3¢ or 5¢-CAACTAAC-3¢ sequences, derived from the promoter regions of the endosperm-speci®c genes Hor2 and Itr1, encoding a B-hordein and trypsin-inhibitor BTI-CMe, respectively. Binding is prevented when these motifs are mutated to 5¢-TgACAAg-3¢ and 5¢-CgACTgAC-3¢. Transient expression experiments in co-bombarded developing endosperms demonstrate that HvGAMYB trans-activates transcription from native Hor2 and Itr1 promoters through binding to the intact motifs described above. Trans-activation of the Hor2 promoter also requires an intact prolamine box (PB). This suggests that HvGAMYB interacts in developing barley endosperms with the PB-binding factor BPBF, an endosperm-speci®c DOF transcriptional activator of the Hor2 gene. The in vivo interaction experiment between HvGAMYB and BPBF was done in the yeast twohybrid system, where HvGAMYB potentiates the BPBF trans-activation capacity through interaction with its C-terminal domain.

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bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm.

Cited by 4 publications

References 17 publications

Colocation between a gene encoding the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat (Triticum aestivum)

Colocation between a gene encoding the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat (Triticum aestivum)

HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination

The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm‐specific genes during seed development

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