Butyrate induces reactive oxygen species production and affects cell cycle progression in human gingival fibroblasts

Chang, Mei‐Chi; Tsai, Yao-Chen; Chen, Yi-Wen; Chan, Chiu‐Po; Huang, Chih‐Fen; Lan, Wan–Hong; Lin, Chung Chih; Jeng, Jiiang‐Huei

doi:10.1111/j.1600-0765.2012.01504.x

Cited by 77 publications

(72 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S4a). Butyrate in the oral cavity is suggested to play a role in the development of periodontal disease [56,57]. Members of the genus Megasphaera are known to produce butyrate [58,59].…”

Section: Discussionmentioning

confidence: 99%

Stability and Resilience of Oral Microcosms Toward Acidification and Candida Outgrowth by Arginine Supplementation

Koopman

Röling²,

Buijs

et al. 2014

Microb Ecol

View full text Add to dashboard Cite

Dysbiosis induced by low pH in the oral ecosystem can lead to caries, a prevalent bacterial disease in humans. The amino acid arginine is one of the pH-elevating agents in the oral cavity. To obtain insights into the effect of arginine on oral microbial ecology, a multi-plaque "artificial mouth" (MAM) biofilm model was inoculated with saliva from a healthy volunteer and microcosms were grown for 4 weeks with 1.6 % (w/v) arginine supplement (Arginine) or without (Control), samples were taken at several time-points. A cariogenic environment was mimicked by sucrose pulsing. The bacterial composition was determined by 16S rRNA gene amplicon sequencing, the presence and amount of Candida and arginine deiminase system genes arcA and sagP by qPCR. Additionally, ammonium and short-chain fatty acid concentrations were determined. The Arginine microcosms were dominated by Streptococcus, Veillonella, and Neisseria and remained stable in time, while the composition of the Control microcosms diverged significantly in time, partially due to the presence of Megasphaera. The percentage of Candida increased 100-fold in the Control microcosms compared to the Arginine microcosms. The pH-raising effect of arginine was confirmed by the pH and ammonium results. The abundances of sagP and arcA were highest in the Arginine microcosms, while the concentration of butyrate was higher in the Control microcosms. We demonstrate that supplementation with arginine serves a health-promoting function; it enhances microcosm resilience toward acidification and suppresses outgrowth of the opportunistic pathogen Candida. Arginine facilitates stability of oral microbial communities and prevents them from becoming cariogenic.

show abstract

“…S4a). Butyrate in the oral cavity is suggested to play a role in the development of periodontal disease [56,57]. Members of the genus Megasphaera are known to produce butyrate [58,59].…”

Section: Discussionmentioning

confidence: 99%

Stability and Resilience of Oral Microcosms Toward Acidification and Candida Outgrowth by Arginine Supplementation

Koopman

Röling²,

Buijs

et al. 2014

Microb Ecol

View full text Add to dashboard Cite

show abstract

“…Short-chain fatty acids can disrupt host defense systems by different mechanisms, such as induction of apoptosis in immune cells (Stehle et al, 2001;Abe, 2012) and gingival epithelial cells (Tsuda et al, 2010) and alteration of cell function and gene expression in human gingival fibroblasts (Chang et al, 2013). Previous studies have demonstrated higher concentrations of short-chain fatty acids in the gingival crevicular fluid of periodontitis patients (Lu et al, 2008;Qiqiang et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic and functional gene structure shifts of the oral microbiomes in periodontitis patients

et al. 2014

The ISME Journal

150

126

View full text Add to dashboard Cite

Determining the composition and function of subgingival dental plaque is crucial to understanding human periodontal health and disease, but it is challenging because of the complexity of the interactions between human microbiomes and human body. Here, we examined the phylogenetic and functional gene differences between periodontal and healthy individuals using MiSeq sequencing of 16S rRNA gene amplicons and a specific functional gene array (a combination of GeoChip 4.0 for biogeochemical processes and HuMiChip 1.0 for human microbiomes). Our analyses indicated that the phylogenetic and functional gene structure of the oral microbiomes were distinctly different between periodontal and healthy groups. Also, 16S rRNA gene sequencing analysis indicated that 39 genera were significantly different between healthy and periodontitis groups, and Fusobacterium, Porphyromonas, Treponema, Filifactor, Eubacterium, Tannerella, Hallella, Parvimonas, Peptostreptococcus and Catonella showed higher relative abundances in the periodontitis group. In addition, functional gene array data showed that a lower gene number but higher signal intensity of major genes existed in periodontitis, and a variety of genes involved in virulence factors, amino acid metabolism and glycosaminoglycan and pyrimidine degradation were enriched in periodontitis, suggesting their potential importance in periodontal pathogenesis. However, the genes involved in amino acid synthesis and pyrimidine synthesis exhibited a significantly lower relative abundance compared with healthy group. Overall, this study provides new insights into our understanding of phylogenetic and functional gene structure of subgingival microbial communities of periodontal patients and their importance in pathogenesis of periodontitis.

show abstract

“…Periodontal pathogens produce butyrate and recently, butyrate was shown to participate in periodontitis development by inhibiting gingival fibroblast cell growth. Butyrate toxicity against human fibroblasts was attributed to increased reactive oxygen species produced by pathogenic bacteria (34). Other than butyrate, acetic acid and succinic acid were also found to be related to gingival inflammation (35).…”

Section: Discussionmentioning

confidence: 99%

Growth behavior of eikenella corrodens and streptococcus gordonii in response to a short chain fatty acid metabolite-acetic acid

Yüce¹,

Tülü²,

Inis³

et al. 2017

J Turgut Ozal Med Cent

View full text Add to dashboard Cite

Aim: Periodontal diseases are chronic, inflammatory and infectious diseases. Therefore, periodontal treatment aims to eliminate periodontopathogenic bacteria causing periodontal diseases. The aim of present study was to evaluate the effect of a bacterial end metabolite, acetic acid, on periodontopathogenic bacteria, Streptococcus gordonii and Eikenella corrodens. Material and Method: In present research, Eikenella corrodens (ATCC® 23834™) and Streptococcus gordonii (NCTC 7870) were tested. Acetic acid was used in 5% concentration dissolved in distilled water. Negative control agent was distilled water and positive control agents were 0.012% chlorhexidine, penicillin, tetracycline and ciprofloxacin. The antibacterial efficacy of acetic acid against bacteria was tested via disc-diffusion method, MIC test and minimum bactericidal concentration tests. Results: The inhibition zone of ciprofloxacin, penicillin, tetracycline, CHX and acetic acid against Eikenella corrodens and Streptococcus gordonii were 32 and 37 mm, 16 and 14 mm, 21 and 16 mm, 13 and 17 mm, and 14 and 11 mm respectively. Ciprofloxacin and penicillin inhibited bacterial growth in MIC and MBC tests against both bacteria. MIC tests of acetic acid and chlorhexidine against Eikenella corrodens revealed inhibitory effect at 7.81 µl/mL and 0.97 µl/mL concentrations, respectively. Against Streptococcus gordonii, MIC of acetic acid and chlorhexidine were 1.95 µl/mL and 3.90 µl/mL, respectively. Conclusion: Acetic acid is a bacterial end product and has a daily consumption as vinegar. Due to the antibacterial efficacy against periodontopathogenic bacteria, it can be useful in adjunction to periodontal treatment. Further studies to evaluate clinical use of acetic acid as mouthwash, dentifrice, gel and/or irrigation agent are necessary.

show abstract

Butyrate induces reactive oxygen species production and affects cell cycle progression in human gingival fibroblasts

Cited by 77 publications

References 36 publications

Stability and Resilience of Oral Microcosms Toward Acidification and Candida Outgrowth by Arginine Supplementation

Stability and Resilience of Oral Microcosms Toward Acidification and Candida Outgrowth by Arginine Supplementation

Phylogenetic and functional gene structure shifts of the oral microbiomes in periodontitis patients

Growth behavior of eikenella corrodens and streptococcus gordonii in response to a short chain fatty acid metabolite-acetic acid

Contact Info

Product

Resources

About