2002
DOI: 10.1590/s0100-879x2002000200004
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Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor and nitric oxide

Abstract: We demonstrated that 4 mM butyrate induces apoptosis in murine peritoneal macrophages in a dose-and time-dependent manner as indicated by studies of cell viability, flow cytometric analysis of annexin-V binding, DNA ladder pattern and the determination of hypodiploid DNA content. The activity of caspase-3 was enhanced during macrophage apoptosis induced by butyrate and the caspase inhibitor z-VAD-FMK (100 µM) inhibited the butyrate effect, indicating the major role of the caspase cascade in the process. The le… Show more

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Cited by 24 publications
(13 citation statements)
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“…The ability of butyrate to induce apoptosis has been previously described in several cell types including cells of the immune system, such as murine thymocytes, splenic T cells, human Jurkat T cells (Kurita-Ochiai et al, 1997) and lymphocytic leukemia cells (Santini et al, 1999). The mechanism by which butyrate induces cell death in different cell types are not known, but there is evidence that butyrate triggers the activation of the caspase cascade in cancer cells leading to their death by apoptosis (Medina et al, 1997;Ramos et al, 2002;Ruemmele et al, 1999). Other authors have also demonstrated that high butyrate concentrations induce caspase-3 activation in peritoneal macrophages (Ramos et al, 2002) or in peripheral blood mononuclear cells (Kurita-Ochiai et al, 1999).…”
Section: Discussionmentioning
confidence: 99%
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“…The ability of butyrate to induce apoptosis has been previously described in several cell types including cells of the immune system, such as murine thymocytes, splenic T cells, human Jurkat T cells (Kurita-Ochiai et al, 1997) and lymphocytic leukemia cells (Santini et al, 1999). The mechanism by which butyrate induces cell death in different cell types are not known, but there is evidence that butyrate triggers the activation of the caspase cascade in cancer cells leading to their death by apoptosis (Medina et al, 1997;Ramos et al, 2002;Ruemmele et al, 1999). Other authors have also demonstrated that high butyrate concentrations induce caspase-3 activation in peritoneal macrophages (Ramos et al, 2002) or in peripheral blood mononuclear cells (Kurita-Ochiai et al, 1999).…”
Section: Discussionmentioning
confidence: 99%
“…We examined the influence of butyrate on T-lymphocyte viability since previous studies have reported the ability of butyrate to induce apoptosis in different cultured cells (Comalada et al, 2006;McBain et al, 1997;Medina et al, 1997;Ramos et al, 2002). In this sense, both dead/viable cell counting (Fig.…”
Section: Butyrate Induces Apoptosis In Con A-activated T Lymphocytesmentioning
confidence: 99%
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“…Butyrate has been shown to induce a number of effects in normal [3,4] as well as transformed cells [5]. These effects include arrest of cell division, induction of cell differentiation, and alteration of gene expression and apoptosis [6].…”
Section: Introductionmentioning
confidence: 99%
“…Il-10, Il-6, Il-2, and TNF-α), 20 but also affect the ability of leukocytes to migrate to the foci of inflammation and to destroy microbial pathogens. 18,21 Furthermore, SCFAs can also lead to apoptosis of neutrophils, 22 macrophages, 23 and lymphocytes. 24,25 The effect may come about as the result of the inflammatory process and the immune response to gut microbiota, which produce SCFAs, these capable of exerting an anti-inflammatory action.…”
Section: Gut Microbiota: Definitionmentioning
confidence: 99%