Burkholderia pseudomallei Biofilm Promotes Adhesion, Internalization and Stimulates Proinflammatory Cytokines in Human Epithelial A549 Cells

Kunyanee, Chanikarn; Kamjumphol, Watcharaporn; Taweechaisupapong, Suwimol; Kanthawong, Sakawrat; Wongwajana, Suwin; Wongratanacheewin, Surasakdi; Hahnvajanawong, Chariya; Chareonsudjai, Sorujsiri

doi:10.1371/journal.pone.0160741

Cited by 33 publications

(58 citation statements)

References 49 publications

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“…It is possible that B. pseudomallei adaptation might employ biofilm formation for survival [ 61 ]. This concurs with a previous study that found that the biofilm of B. pseudomallei increased bacterial adherence to host cells [ 75 ]. The T6SS genes, which encode proteins that facilitate cell-to-cell spreading, are reportedly induced by iron deprivation [ 76 ].…”

Section: Molecular Mechanisms For B Pseudomallei supporting

confidence: 93%

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Duangurai

Indrawattana

Pumirat

2018

BioMed Research International

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Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, which can be fatal in humans. Melioidosis is prevalent in the tropical regions of Southeast Asia and Northern Australia. Ecological data have shown that this bacterium can survive as a free-living organism in environmental niches, such as soil and water, as well as a parasite living in host organisms, such as ameba, plants, fungi, and animals. This review provides an overview of the survival and adaptation of B. pseudomallei to stressful conditions induced by hostile environmental factors, such as salinity, oxidation, and iron levels. The adaptation of B. pseudomallei in host cells is also reviewed. The adaptive survival mechanisms of this pathogen mainly involve modulation of gene and protein expression, which could cause alterations in the bacteria's cell membrane, metabolism, and virulence. Understanding the adaptations of this organism to environmental factors provides important insights into the survival and pathogenesis of B. pseudomallei, which may lead to the development of novel strategies for the control, prevention, and treatment of melioidosis in the future.

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Section: Molecular Mechanisms For B Pseudomallei supporting

confidence: 93%

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Duangurai

Indrawattana

Pumirat

2018

BioMed Research International

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“…Potential bacterial toxicity may be enhanced when cells are present in biofilms, which are heterogeneously sessile bacterial communities that adhere to each other and to solid surfaces (Wang et al, 2012 ). Kunyanee et al ( 2016 ) reported the important role of Burkholderia pseudomallei biofilms in bacterial pathogenesis in human epithelial cells with respect to initial attachment and invasion. In the Δ bcsR mutant of C. sakazakii , the decrease of biofilm formation suggests that this gene may affect adhesion/invasion by regulating biofilm synthesis.…”

Section: Discussionmentioning

confidence: 99%

A Negative Regulator of Cellulose Biosynthesis, bcsR, Affects Biofilm Formation, and Adhesion/Invasion Ability of Cronobacter sakazakii

Gao

et al. 2017

Front. Microbiol.

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Cronobacter sakazakii is an important foodborne pathogen that causes neonatal meningitis and sepsis, with high mortality in neonates. However, very little information is available regarding the pathogenesis of C. sakazakii at the genetic level. In our previous study, a cellulose biosynthesis-related gene (bcsR) was shown to be involved in C. sakazakii adhesion/invasion into epithelial cells. In this study, the detailed functions of this gene were investigated using a gene knockout technique. A bcsR knockout mutant (ΔbcsR) of C. sakazakii ATCC BAA-894 showed decreased adhesion/invasion (3.9-fold) in human epithelial cell line HCT-8. Biofilm formation by the mutant was reduced to 50% of that exhibited by the wild-type (WT) strain. Raman spectrometry was used to detect variations in biofilm components caused by bcsR knockout, and certain components, including carotenoids, fatty acids, and amides, were significantly reduced. However, another biofilm component, cellulose, was increased in ΔbcsR, suggesting that bcsR negatively affects cellulose biosynthesis. This result was also verified via RT-PCR, which demonstrated up-regulation of five crucial cellulose synthesis genes (bcsA, B, C, E, Q) in ΔbcsR. Furthermore, the expression of other virulence or biofilm-related genes, including flagellar assembly genes (fliA, C, D) and toxicity-related genes (ompA, ompX, hfq), was studied. The expression of fliC and ompA in the ΔbcsR mutant was found to be remarkably reduced compared with that in the wild-type and the others were also affected excepted ompX. In summary, bcsR is a negative regulator of cellulose biosynthesis but positively regulates biofilm formation and the adhesion/invasion ability of C. sakazakii.

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“…Virulence factors like flagellin, Type III Secretion Systems (T3SS) [ 7 ], adhesins [ 5 ], pili [ 5 ], and biofilm [ 8 ] have all been described for B. pseudomallei and B. mallei. Actin-based motility [ 7 ] and flagellae permit rapid transit through cells.…”

Section: Discussionmentioning

confidence: 99%

Multiple recurrent abscesses in a patient with undiagnosed IL-12 deficiency and infection by Burkholderia gladioli

Vigliani

Cunha

2018

IDCases

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We report the occurrence of two severe illnesses experienced by one patient over a 19 year period of time. Both illnesses were characterized by severe inflammation and tissue destruction. Signs and symptoms of the first illness were characteristic of lymphogranuloma venereum (LGV). The second illness mimicked scrofula. During the second illness the patient was discovered to have a rare immunodeficiency due to auto-antibodies to Interleukin (IL)-12 and infection by Burkholderia gladioli, a plant pathogen usually harmless in humans.We were able to retrieve biopsies from the first illness to establish that B. gladioli was already present during the original presentation. That first illness lasted 5 year s, but she survived without the correct pathogen ever being identified, and without a diagnosis of immunodeficiency. After a remission of 10 year s, she experienced her second illness.The responses to treatment before and after the correct diagnoses were established provide us with an excellent opportunity to consider and discuss how disease expression reflects complex relationships between host defenses and microbial characteristics.

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Burkholderia pseudomallei Biofilm Promotes Adhesion, Internalization and Stimulates Proinflammatory Cytokines in Human Epithelial A549 Cells

Cited by 33 publications

References 49 publications

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

A Negative Regulator of Cellulose Biosynthesis, bcsR, Affects Biofilm Formation, and Adhesion/Invasion Ability of Cronobacter sakazakii

Multiple recurrent abscesses in a patient with undiagnosed IL-12 deficiency and infection by Burkholderia gladioli

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