Buprofezin dissipation and safety assessment in open field cabbage and cauliflower using GC/ITMS employing an analyte protectant

Mandal

et al. 2021

Preprint

The present study was conducted to investigate the residue status of two insecticides (acetamiprid and buprofezin) and their dissipation kinetics in three matrices viz. paddy grain, straw and soil. The extraction protocol for residues of these two insecticides was executed using acetonitrile solvent. The analytical method was validated, which showed good linearity with the limit of quantification (LOQ) value of 0.01 and 0.02 mg kg− 1 for acetamiprid and buprofezin respectively. The recovery range was 79.67–98.33 % concerning all the matrices in both the insecticides. Acetamiprid (20% SP) and Buprofezin (25% SC) were applied separately in the paddy field in two doses: single dose (recommended dose) and double dose along with untreated control throughout the experiment. Residue analysis of these two insecticides in paddy (grain and straw) and soil was accomplished employing high-pressure liquid chromatography (HPLC) with a UV detector. The dissipation data showed that acetamiprid exhibited higher dissipation in comparison with buprofezin. However, their persistence was found slightly higher in soil. The dissipation dynamics in the rice and soil were discussed with biological half-lives of both the insecticides. Consumer risk assessment study was also made considering its fate to the consumers.

Section: Dissipation Kinetics and Waiting Periodsmentioning

confidence: 99%

A Liquid Chromatographic Method for Determination of Acetamiprid and Buprofezin Residues and Their Dissipation Kinetics in Paddy Matrices and Soil

Mandal

et al. 2021

Preprint

Section: Introductionmentioning

confidence: 99%

“…Nihon Nohyaku Co., Ltd. (Tokyo, Japan) developed buprofezin for registration in 1983, 1 which is an insect growth regulator (IGR). 1 Its chemical formula is C 16 H 23 N 3 SO, and its relative molecular weight is 305.4. The substance is a white crystal with a melting point of 104.5−105.5 °C and a relative density of 1.18 (25 °C), and it is stable under conditions of acid, alkali, light, and heat.…”

Section: Introductionmentioning

confidence: 99%

“…The instrument detection methods for IGR insecticides mainly include gas chromatography (GC) (GB/T 5009.184− 2003; SN/T 1594−2019), GC with ion trap mass spectrometry (GC/ITMS), 1 high-performance liquid chromatography (HPLC) (Ding et al, 2019), 13 mass spectrometry (GC−MS/MS), 14 and liquid chromatography-tandem mass spectrometry (HPLC−MS/MS). 15 Confirmatory analysis methods provide better sensitivity, precision, and accuracy but are expensive and have slow analysis speeds as sample preparation is complex.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Generating Monoclonal Antibodies against Buprofezin and Developing Immunoassays for Its Residue Detection in Tea Samples

Xu,

Abd El Aty,

Cao

et al. 2023

J. Agric. Food Chem.

The synthesis of a hapten and antigen for the preparation of a monoclonal antibody (mAb) for buprofezin is described. The recognition mechanism of hapten and buprofezin by monoclonal antibodies (mAb-19F2) is described. The effectiveness of the mAb-19F2 immunoassay technique was assessed, and the effective detection of buprofezin in tea samples was achieved through the establishment of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatography assay (GICA). The mAb-19F2 subtype was IgG 1 , with an IC 50 of 1.8 ng/mL and a linear range (IC 20 − IC 80 ) of 0.6−5.4 μg/L, and had a cross-reaction rate of less than 0.18% with 29 other pesticides (neonicotinoids and insect growth regulators). The study identified π−π stacking interactions between hapten and TYR-61 at the mAb-19F2 site and alkyl/phosphate interactions with TRP-105 and ARG-103. The ic-ELISA had an IC 50 of 12.9 ng/mL in green tea and 5.65 ng/mL in black tea, with a recovery rate of 92.4%−101.0% and RSD of 2.1%−4.8%. The GICA had a limit of detection (LOD) was 500 ng/mL, with the complete disappearance of the test lines visible to the naked eye. The limit of quantitation (LOQ, IC 20 ) was determined to be 16.8 ng/mL. Additionally, the developed GICA showed no cross-reactivity with neonicotinoid pesticides. The recovery rate of tea spiked recovered samples was 83.6%−92.2%, with an RSD of 5.3%−12.6%, and the results were consistent with the LC/MS method. This study is important for the real-time detection of buprofezin residues to ensure food safety and human health.

Biomedical Chromatography

“…The dissipation of pesticides is influenced by several factors, including the plant species, plant growth (dilution), climate (rainfall, sunlight, temperature, etc. ), pesticide application method, plant uptake of the pesticide (via leaves, stem, or roots), and properties of the pesticide (Abdallah, El Agamy, et al, 2019;Abdallah, El-Hamid, & Raheem, 2019;Abdallah & Malhat, 2020). The dissipation behavior of myclobutanil, penconazole, triadimenol, and tebuconazole has been investigated individually in various crops under specific climate conditions and cultivation techniques (Abd-Alrahman & Ahmed, 2012;Abd-Alrahman & Ahmed, 2013;Abdella et al, 2015;Cui et al, 2011;Deng et al, 2010;Dong et al, 2010;Dong & Hu, 2014;Dubey et al, 2020;Hassan et al, 2013;Heshmati et al, 2020;Kaur et al, 2012;Liu et al, 2009;Liu et al, 2012;Lou et al, 2008;Lucini & Molinari, 2009;Matadha et al, 2019;Romeh et al, 2009;Saidi et al, 2017;Singh & Singh, 2014).…”

mentioning

confidence: 99%

Levels of residues and dietary risk assessment of the fungicides myclobutanil, penconazole, tebuconazole, and triadimenol in squash

Abdallah

Alrasheed

Al-Mundarij

et al. 2021

Self Cite

Triazole fungicides may potentially harm human health. The 'quick, easy, cheap, effective, rugged, and safe' approach has become popular for extraction and cleanup during pesticide residue analysis. We aimed to (a) validate a method for the simultaneous determination of myclobutanil, penconazole, tebuconazole, and triadimenol in squash using LC-MS/MS and (b) determine the pre-harvest intervals (PHIs) and assess the related risk of consuming squash cultivated under open-field conditions in Saudi Arabia. Using acetonitrile as the extraction solvent and fourfold dilution in deionized water led to weak signal suppression (<À6.1%). The limits of quantitation ranged from 10 to 40 μg/kg. Mean recovery and relative standard deviation ranged from 81.7 to 96.3% and from 3.6 to 11.4%. The half-lives ranged from 2.22 to 3.83 days, and the dissipation followed first-order kinetics. The terminal residues of myclobutanil, penconazole, tebuconazole, and triadimenol were <0.771, <0.307, <0.459, and <0.954 mg/kg, respectively, 7 days after two or three applications of recommended dosages. The PHIs of 7. 1-11.4, 8.7-13.1, 3.8-5.3, and 11.3-14.3 days are suggested after the application of the recommended dose and double the recommended dose. A consumer risk assessment based on estimated dietary intake indicated that the consumption of squash treated with the recommended doses does not pose a significant health risk. K E Y W O R D S dissipation kinetics, LC-MS/MS, risk assessment, squash, triazole fungicides 1 | INTRODUCTION Squash (Cucurbita sp.)-a rich source of nutrients such as carbohydrates, vitamins, and minerals (Roura et al., 2004)-is a major crop in Saudi Arabia, where the plants are cultivated under open-field and greenhouse conditions (FAOSTAT, 2018). Squash and other cucurbit plants are susceptible to powdery mildew disease, which is mainly caused by the fungus Podosphaera xanthii. In severe cases, powdery mildew can cause premature death of the leaves and reduce squash production over the long term (Velkov & Masheva, 2002).At present, powdery mildew infections of squash leaves are routinely treated with the triazole fungicides myclobutanil, penconazole, tebuconazole, and triadimenol (Figure 1). Triazoles are inhibitors of demethylation that disrupt the biosynthesis of fungal cell