1996
DOI: 10.1001/archderm.132.2.151
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Bullous pemphigoid and epidermolysis bullosa acquisita. Differentiation by fluorescence overlay antigen mapping

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Cited by 33 publications
(35 citation statements)
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“…Currently, a diagnosis of EBA should be confirmed by at least one of the following tests, which are only performed in academic centres and are not widely available to the average dermatologist worldwide: 13,15,35 (i) among tests requiring skin or MM biopsieselectron microscopy (EM) and direct IEM, 44 serration pattern analysis by DIF 41,45,46 and fluorescent overlay antigen mapping (FOAM); [47][48][49] and (ii) among serological tests for the detection of circulating autoAbs to Col7enzyme-linked immunosorbent assay (ELISA), IIF on BIOCHIP TM with noncollagenous 1 (NC1) Col7-transfected human cells, 50 immunoblotting (IB), IIF on skin deficient in Col7 51,52 and/or indirect IEM. 44 Obviously, all these serological tests require that the patient with EBA has autoAbs to Col7 circulating in their blood and it must be kept in mind that anti-Col7 autoAbs are also present in bullous systemic lupus erythematosus (BSLE).…”
Section: Laboratory Testing For Epidermolysis Bullosa Acquisitamentioning
confidence: 99%
“…Currently, a diagnosis of EBA should be confirmed by at least one of the following tests, which are only performed in academic centres and are not widely available to the average dermatologist worldwide: 13,15,35 (i) among tests requiring skin or MM biopsieselectron microscopy (EM) and direct IEM, 44 serration pattern analysis by DIF 41,45,46 and fluorescent overlay antigen mapping (FOAM); [47][48][49] and (ii) among serological tests for the detection of circulating autoAbs to Col7enzyme-linked immunosorbent assay (ELISA), IIF on BIOCHIP TM with noncollagenous 1 (NC1) Col7-transfected human cells, 50 immunoblotting (IB), IIF on skin deficient in Col7 51,52 and/or indirect IEM. 44 Obviously, all these serological tests require that the patient with EBA has autoAbs to Col7 circulating in their blood and it must be kept in mind that anti-Col7 autoAbs are also present in bullous systemic lupus erythematosus (BSLE).…”
Section: Laboratory Testing For Epidermolysis Bullosa Acquisitamentioning
confidence: 99%
“…Immunoelectron microscopy is considered the gold standard for EBA diagnosis. However, based on practicability and availability, other methods, for example, u-serrated pattern analysis and the combination of direct IF microscopy with the detection of circulating autoantibodies, are more frequently used [199, 200]. …”
Section: Diagnosis Of Ebamentioning
confidence: 99%
“…This technique is able to distinguish IgG deposits that are above the lamina densa, as seen in BP patients, from those that are below the lamina densa, as seen in EBA patients [70, 71]. FOAM is able to demonstrate different antigens of the BMZ by using monoclonal antibodies on perilesional skin from patients with autoimmune skin diseases to the different components of the BMZ, such as C7.…”
Section: Laboratory Testsmentioning
confidence: 99%