Bud-Derivatives, a Novel Source of Polyphenols and How Different Extraction Processes Affect Their Composition

Abstract

The use of herbal food supplements, as a concentrate form of vegetable extracts, increased so much over the past years to count them among the relevant sources of dietetic polyphenols. Bud-derivatives are a category of botanicals perceived as a “new entry” in this sector since they are still poorly studied. Due to the lack of a manufacturing process specification, very different products can be found on the market in terms of their polyphenolic profile depending on the experimental conditions of manufacturing. In this research two different manufacturing processes, using two different protocols, and eight species (Carpinus betulus L., Cornus mas L., Ficus carica L., Fraxinus excelsior L., Larix decidua Mill., Pinus montana Mill., Quercus petraea (Matt.) Liebl., Tilia tomentosa Moench), commonly used to produce bud-derivatives, have been considered as a case study. An untargeted spectroscopic fingerprint of the extracts, coupled to chemometrics, provide to be a useful tool to identify these botanicals. The targeted phytochemical fingerprint by HPLC provided a screening of the main bud-derivatives polyphenolic classes highlighting a high variability depending on both method and protocol used. Nevertheless, ultrasonic extraction proved to be less sensitive to the different extraction protocols than conventional maceration regarding the extract polyphenolic profile.

Comparative Evaluation of Four Extraction Methods of Antioxidant Compounds from Decatropis bicolor in Aqueous Medium Applying Response Surface Design

“…The most common methods used for the extraction of bioactive compounds from vegetal matrixes (plants, vegetables, fruits, and by-products), are maceration, Soxhlet [ 1 , 2 ], and conventional solid-liquid extraction [ 10 ], although shaking and heated reflux have also been used [ 1 , 10 ]. Other methods as microwave, microwave-assisted [ 11 , 12 , 13 , 14 ], ultrasound-assisted [ 1 , 2 , 14 , 15 , 16 , 17 , 18 ], and super-critical fluid extraction [ 1 , 2 , 14 , 18 ] have been used to increase extraction yield [ 2 ]. The efficiency of the extraction depends on several factors such as temperature, sample (the type of plant and pre-conditioning), time, and the solvent agent, among others.…”

Vitis vinifera L. Pruning Waste for Bud-Preparations as Source of Phenolic Compounds–Traditional and Innovative Extraction Techniques to Produce New Natural Products

“…However, these products are still poorly studied, even if some in vitro/in vivo studies for veterinary and human use have been already reported [1][2][3]. Moreover, the high number of herbal products on the botanical market and an almost absence of specific legislation to control their quality and safety causes many issues on these products (e.g., adulteration, falsification, and fraud) [4].…”

A Study of the Interaction, Morphology, and Structure in Trypsin-Epigallocatechin-3-Gallate Complexes

“…Meanwhile, binding to proteins may affect the absorption of polyphenol compounds and reduces their bioavailability and nutritional benefits [ 12 ]. Factors such as the structures of polyphenols and proteins and the ratio of polyphenol/protein can affect the mode of interaction between proteins and polyphenols [ 13 ]. The interaction between polyphenols and several enzymes such as tyrosinase, amylase, xanthine oxidase, and peroxidase has been reported [ 14 ].…”