2012
DOI: 10.4236/abb.2012.31012
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Abstract: Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the … Show more

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Cited by 3 publications
(5 citation statements)
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“…Plasmid carrying the bls gene injected in to Balb C mice induced both humoral and cellular immune responses (Velikovsky et al, 2003). Bellido et al, 2012 was able to use BLS as an immuno modulator to enhance the antibody response in hens in order to increase egg yolk antibodies. BLS has been reported as a carrier for antigen delivery (Sciutto et al, 2005), based on its physicochemical and immunogenic properties (Zylberman et al, 2004).…”
Section: Resultsmentioning
confidence: 99%
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“…Plasmid carrying the bls gene injected in to Balb C mice induced both humoral and cellular immune responses (Velikovsky et al, 2003). Bellido et al, 2012 was able to use BLS as an immuno modulator to enhance the antibody response in hens in order to increase egg yolk antibodies. BLS has been reported as a carrier for antigen delivery (Sciutto et al, 2005), based on its physicochemical and immunogenic properties (Zylberman et al, 2004).…”
Section: Resultsmentioning
confidence: 99%
“…Rosas et al, (2006) showed that BLS can be used as both an antigen-carrier and as an adjuvant in the design of new oral subunit vaccines. There are other reports of using BLS as an immuno modulator to enhance the antibody response in hens (Bellido et al, 2012). Hence, the recombinant BLS protein expressed in E. coli can be exploited as diagnostic antigen or immunogen for development of immuno assay and vaccine for brucellosis.…”
Section: Resultsmentioning
confidence: 99%
“…Based on our previous work, BLS was coupled to VP8d, a shorter fragment of VP8 ∗ , and transplastomic BLSVP8d plants were obtained. Since properly folded BLSVP8d had already been produced in bacteria and its potent immunomodulatory properties had already been demonstrated in female mice and laying hen models, it was a good candidate to further analyze its immunogenic properties in a recombinant plant system ( Bellido et al, 2009 , 2012 ).…”
Section: Discussionmentioning
confidence: 99%
“…The supernatant was stored at 4°C and used for IgY analysis. Titers of IgY against BLSVP8d were determined according to Bellido et al (2012) . Briefly, 1 μg of purified VP8d was directly adsorbed onto each well overnight in carbonate-bicarbonate buffer pH 9.6 and blocked with PBS-T, 5% normal horse serum for 1 h at 37°C.…”
Section: Methodsmentioning
confidence: 99%
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