Bruceine D Acts as a Potential Insecticide by Antagonizing 20E–EcR/USP Signal Transduction

Chen, Dongping; Li, Kang; Wang, Bingfeng; Chen, Huimin; Jiang, Heng; Zhao, Chen; Yao, Guangkai; Li, Sheng; Xu, Hanhong

doi:10.1021/acs.jafc.3c02275

Cited by 5 publications

(5 citation statements)

References 47 publications

(81 reference statements)

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“…javanica (L.) Merr. have been reported to be due to the presence of Bruceine D and other substances effectively controlling agricultural pests such as the DBM. , According to our findings, incorporating BJO leads to a significant enhancement in the insecticidal effect of the β-CYP EC emulsion against DBM. The enhancement can be attributed to the presence of insecticidally active compounds, such as Bruceine D, in BJO, along with the superior leaf-wetting properties of β-CYP EC-2, enabling the leaves to accommodate a greater amount of β-CYP.…”

Section: Resultssupporting

confidence: 65%

Effect of Brucea javanica Oil on the Toxicity of β-Cypermethrin Emulsifiable Concentrate Formulation

Wu,

Yang,

Zheng

et al. 2024

ACS Appl. Mater. Interfaces

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Enhancing the performance of traditional pesticide formulations by improving their leaf surface wetting capabilities is a crucial approach for maximizing the pesticide efficiency. This study develops an emulsifiable concentrate (EC) of 4.5% β-cypermethrin containing Brucea javanica oil (BJO). The incorporation of BJO aims to improve the leaf-wetting properties of the EC formulation and enhance its insecticidal effectiveness. The droplet size and emulsion characteristics of β-CYP EC emulsion with varying concentrations of the emulsifier were evaluated, and changes after incorporating BJO were assessed to develop the optimal formulation. A comprehensive comparison was conducted among commercial 4.5% β-cypermethrin EC (β-CYP EC-1), 4.5% βcypermethrin EC with BJO (β-CYP EC-2), and 4.5% βcypermethrin EC without BJO (β-CYP EC-3). This comparison encompassed various factors including storage stability, insecticidal activity, cytotoxicity, and wetting performance on cabbage leaves. The results indicated that the ideal emulsifier concentration was 15% emulsifier 0201B. β-CYP EC-2 demonstrated superior wetting properties on cabbage leaves (the wetting performance of β-CYP EC-2 emulsion on cabbage leaves is 2.60 times that of the β-CYP EC-1 emulsion), heightened insecticidal activity against the third larvae of Plutella xylostella [diamondback moth (DBM)] [the insecticidal activity of the β-CYP EC-2 emulsion against the third larvae of DBM is 1.93 times that of the β-CYP EC-1 emulsion (12 h)], and more obvious inhibitory effects on the proliferation of DBM embryo cells than the other tested formulations. These findings have significant implications for advancing pest control strategies and promoting sustainable and effective agricultural practices.

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Section: Resultssupporting

confidence: 65%

Effect of Brucea javanica Oil on the Toxicity of β-Cypermethrin Emulsifiable Concentrate Formulation

Wu,

Yang,

Zheng

et al. 2024

ACS Appl. Mater. Interfaces

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“…According to the electrophysiological experiments, Bruceine D demonstrated observable inhibition of the PxRDL1 receptor. Bruceine D demonstrated notable inhibitory effects on the PxRDL1 receptor 31 …”

Section: Discussionmentioning

confidence: 98%

“…In this study, we provide a perspective to explain the mechanism of growth inhibition activity focusing on the perspective of nutrient absorption and assimilation. Coincidentally, the latest reports revealed that bruceine D can inhibit insect growth and metamorphosis through inhibition of the expression of 20E response genes by antagonizing 20E−EcR/USP signal transduction 31 . This might explain the reason for disrupting the titer of ecdysone and juvenile hormone.…”

Section: Discussionmentioning

confidence: 99%

“…Coincidentally, the latest reports revealed that bruceine D can inhibit insect growth and metamorphosis through inhibition of the expression of 20E response genes by antagonizing 20E−EcR/USP signal transduction. 31 This might explain the reason for disrupting the titer of ecdysone and juvenile hormone. Further analysis of the transcriptomics and metabolomics showed bruceine D also acts on nervous system, which contributes to feeding deterrents.…”

Section: Discussionmentioning

confidence: 99%

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Bruceine D inhibits the growth of Spodoptera litura by inducing cell apoptosis in the midgut via an oxidative burst

Mao,

Lu,

et al. 2024

Pest Management Science

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BACKGROUNDSpodoptera litura is one of the most harmful lepidoptera pests in China, which is difficult to control due to its strong resistance to the current frequently‐used insecticide species. Requirement of developing pesticides with novel toxicology mechanisms to control S. litura is urgent. The quassinoid of bruceine D display outstanding systemic properties and strong insecticidal activity against S. litura, which possess notable application potential for integrative management of S. litura. But the mechanism of toxicity remains unclear.RESULTSIn this study, we found that bruceine D exerts potent growth inhibitory activity against S. litura, disrupt the ecdysone and juvenile hormone titers, and causing long‐term adverse effects. Association analysis between transcriptomics and metabolomics suggested that bruceine D affected the digestion and absorption capacity of S. litura larvae by inducing a strong oxidative stress response and cell apoptosis in the intestine. Further analysis demonstrated that bruceine D can inhibit the activities of digestive and antioxidant enzymes and induce MDA and ROS overaccumulation in the midgut. Moreover, the protein level of Bax, cleavage caspase 3, and cytochrome c expressed in cytoplasm (cyto) were up‐regulated by bruceine D, while Bcl‐2 and cytochrome c expressed in mitochondria (mito) were down‐regulated. In addition, there was a noticeable increase in caspase‐3 protease activity. Histopathological observations revealed that bruceine D damages the structure of midgut epithelial cells and activates lysosomes, which subsequently disrupts the midgut tissue.CONCLUSIONOverall, our findings suggested that bruceine D induced excessive ROS accumulation in midgut epithelial cells. The resulting cell apoptosis disrupted midgut tissue, leading ultimately to reduced nutrient digestion and absorption in the midgut and the inhibition of larval growth.This article is protected by copyright. All rights reserved.

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“…javanica Merr is a shrub and grows primarily in the wet tropical biome from tropical and subtropical Asia to North Australia. The typical active ingredients of the genus Brucea are quassinoids that exhibit various biological activities including antibacterial, antimalarial, antitubercular, anti-HIV, and anticancer activities . Currently, there are more than 10 quassinoids isolated, of which Bruceine A, C, D, and H, BR, and dehydrobrucatol have been found to display potent anticancer activities such as inhibiting cell proliferation, inducing apoptosis, inhibiting migration/invasion, inducing autophagy, and restraining angiogenesis .…”

Section: Discussionmentioning

confidence: 99%

Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf

Li,

Zhu,

Sun

et al. 2023

ACS Pharmacol. Transl. Sci.

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The oncogenic transcription factor c-Maf has been proposed as an ideal therapeutic target for multiple myeloma (MM), a not-yet-curable malignancy of plasma cells. In the present study, we establish a c-Maf-based luciferase screen system and apply it to screen a homemade library composed of natural products from which bruceine B (BB) is identified to display potent antimyeloma activity. BB is a key ingredient isolated from the Chinese traditional medicinal plant Brucea javanica (L.) Merr. (Simaroubaceae). BB inhibits MM cell proliferation and induces MM cell apoptosis in a caspase-3-dependent manner. The mechanism studies showed that BB inhibits c-Maf transcriptional activity and downregulates the expression of CCND2 and ITGB7, the downstream genes typically modulated by c-Maf. Moreover, BB induces c-Maf degradation via proteasomes by inducing c-Maf for K48-linked polyubiquitination in association with downregulated Otub1 and USP5, two proven deubiquitinases of c-Maf. We also found that c-Maf activates STAT3 and BB suppresses the STAT3 signaling. In the in vivo study, BB displays potent antimyeloma activity and almost suppresses the growth of myeloma xenografts in 7 days but shows no overt toxicity to mice. In conclusion, this study identifies BB as a novel inhibitor of c-Maf by promoting its degradation via the ubiquitin-proteasomal pathway. Given the safety and the successful clinical application of bruceine products in traditional medicine, BB is ensured for further investigation for the treatment of patients with MM.

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Bruceine D Acts as a Potential Insecticide by Antagonizing 20E–EcR/USP Signal Transduction

Cited by 5 publications

References 47 publications

Effect of Brucea javanica Oil on the Toxicity of β-Cypermethrin Emulsifiable Concentrate Formulation

Effect of Brucea javanica Oil on the Toxicity of β-Cypermethrin Emulsifiable Concentrate Formulation

Bruceine D inhibits the growth of Spodoptera litura by inducing cell apoptosis in the midgut via an oxidative burst

Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf

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