Brown Norway rat model of food allergy: Effect of plant components on the development of oral sensitization

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

“…These results indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable with that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans (Atkinson et al 1996).…”

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

“…These results indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable with that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans (Atkinson et al 1996).…”

ISCOMs: an adjuvant with multiple functions

“…The authors' conclusion was that there was no correlation between hemolytic activity, lethality, and adjuvant activity. No specific IgE was induced with any of these four fractions in contrast to other reports using Quil A [66], Gypsophilia saponins [67], or saponins from un-named sources [68,69] all of which have shown increased levels of IgE after the addition of saponin to vaccines.…”

Assessment of Protein Allergenicity

“…These studies indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable to that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans 15. Although the induced antibodies in the BN rat apparently react to relevant proteins compared to the human situation, it remains to be elucidated whether the induced specific antibodies in the rat react to the same epitopes as the antibodies in the sera from patients.…”

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

“…These results indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable with that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans (Atkinson et al 1996).…”

ISCOMs: an adjuvant with multiple functions

“…The authors' conclusion was that there was no correlation between hemolytic activity, lethality, and adjuvant activity. No specific IgE was induced with any of these four fractions in contrast to other reports using Quil A [66], Gypsophilia saponins [67], or saponins from un-named sources [68,69] all of which have shown increased levels of IgE after the addition of saponin to vaccines.…”

Assessment of Protein Allergenicity

“…These studies indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable to that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans 15. Although the induced antibodies in the BN rat apparently react to relevant proteins compared to the human situation, it remains to be elucidated whether the induced specific antibodies in the rat react to the same epitopes as the antibodies in the sera from patients.…”

Assessment of the allergic potential of food protein extracts and proteins on oral application using the brown Norway rat model.

“…These results indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable with that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans (Atkinson et al 1996).…”

ISCOMs: an adjuvant with multiple functions

“…The authors' conclusion was that there was no correlation between hemolytic activity, lethality, and adjuvant activity. No specific IgE was induced with any of these four fractions in contrast to other reports using Quil A [66], Gypsophilia saponins [67], or saponins from un-named sources [68,69] all of which have shown increased levels of IgE after the addition of saponin to vaccines.…”

Assessment of Protein Allergenicity

“…These studies indicate that upon daily intragastric dosing with HEW proteins or CM proteins, the specific protein recognition of induced antibodies in the BN rat is comparable to that observed in sera from allergic patients. The same phenomenon was described in BN rats intraperitoneally sensitized with CM, which produced a profile of IgE antibodies to milk proteins similar to that observed in humans 15. Although the induced antibodies in the BN rat apparently react to relevant proteins compared to the human situation, it remains to be elucidated whether the induced specific antibodies in the rat react to the same epitopes as the antibodies in the sera from patients.…”