Bronchial circulation angiogenesis in the rat quantified with SPECT and micro-CT

Wietholt, Christian; Roerig, David L.; Gordon, John B.; Haworth, Steven T.; Molthen, Robert C.; Clough, Anne V.

doi:10.1007/s00259-007-0684-y

Cited by 18 publications

(13 citation statements)

References 36 publications

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“…However, with increased pulmonary flow in the right lung, moderate increases lead to larger alveoli, whereas severe increases in pulmonary flow increase the number of alveoli. As shown in our study and those by Haworth et al in pigs (10) Others have made similar observations in adult dogs (21,22,31), rats (17,38,39), and mice (23,37), indicating that, within a prolonged enough period of pulmonary flow reduction, the systemic circulation is recruited to supply the obstructed lung. This recruitment of the systemic circulation may account for the increased density of small arteries observed in the banded left lungs in our study.…”

Section: Discussionsupporting

confidence: 91%

Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs

Razavi¹,

Stewart

Xu³

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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Pulmonary artery (PA) stenosis is a difficult obstructive defect to manage since clinicians cannot know a priori which obstructions to treat and when. Prognosis of PA stenosis and its chronic effects on lung development are poorly understood. This study aimed to characterize the hemodynamic and structural effects of PA stenosis during development. Fourteen male Sprague-Dawley rats underwent left PA (LPA) banding at age 21 days, and 13 underwent sham operation. Hemodynamic and structural impacts were studied longitudinally at 20, 36, 52, 100, and 160 days. Chronic LPA banding resulted in a significant reduction in LPA flow (P < 0.0001) and size of both proximal LPA (P < 0.0001) and distal LPA (P < 0.01), as well as a significant increase in flow and size of the right PA (P < 0.05) throughout development. Flows and sizes adapted such that normal levels of wall shear were restored after banding. At 160 days, LPA banding resulted in a significant decrease in left lung volume and an increase in right lung volume but no significant differences in total lung volume. There was an elevation of proximal LPA pressure as well as right ventricular hypertrophy in the banded animals. The banded lung exhibited arterial disorganization, loss of vessels, and enlargement of its bronchial arteries, whereas the contralateral lung showed signs of vascular pathology. There are consequences on development of both lungs in the presence of an LPA stenosis at young age. These results suggest that early intervention may be necessary to optimize left lung growth and minimize right lung vascular pathology.

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Section: Discussionsupporting

confidence: 91%

Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs

Razavi¹,

Stewart

Xu³

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Vascular perfusion is a common strategy in the evaluation of blood vessel parameters [34]. Recently, angiography with lCT has been successfully used to study blood vessels of the lung, kidneys, and tumors [35,36]. It is also applied in the exploration of blood supply to the bone [37].…”

Section: Discussionmentioning

confidence: 99%

Difference in intraosseous blood vessel volume and number in osteoporotic model mice induced by spinal cord injury and sciatic nerve resection

Ding¹,

Wang²,

Wei³

et al. 2011

J Bone Miner Metab

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In the present study, we examined intraosseous blood vessel parameters of the tibial metaphysis in mice using microcomputed tomography (µCT) to investigate the relationship between post-nerve-injury osteoporosis and local intraosseous blood vessel volume and number. Mice were randomly divided into groups receiving spinal cord injury (SCI), sciatic nerve resection group (NX), or intact controls (30 mice/group). Four weeks after surgery, mice were perfused with silicone and the distribution of intraosseous blood vessels analyzed by μCT. The bone density, μCT microstructure, biomechanical properties, and the immunohistochemical and biochemical indicators of angiogenesis were also measured. The SCI group showed significantly reduced tibial metaphysis bone density, μCT bone microstructure, tibial biomechanical properties, indicators of angiogenesis, and intraosseous blood vessel parameters compared to the NX group. Furthermore, the spinal cord-injured mice exhibited significantly decreased intraosseous blood vessel volume and number during the development of osteoporosis. In conclusion, these data suggest that decreased intraosseous blood vessel volume and number may play an important role in the development of post-nerve-injury osteoporosis.

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“…The CFD method and these models can assist in the development of new inhalation therapies and the optimization of inhaler devices (Coates et al, 2007;Kleinstreuer et al, 2007). Advances in small animal imaging using micro-CT or MRI have made it possible to study the respiratory system in a specific animal into great detail (Johnson, 2007;Lam et al, 2007;Wietholt et al, 2008). Earlier work by Chaturvedi and Lee (Chaturvedi and Lee, 2005) has used micro CT images to accurately study the morphology of the mouse and canine airway tract based on lung casts and segmentation principles.…”

mentioning

confidence: 99%

Study of the Variability in Upper and Lower Airway Morphology in Sprague–Dawley Rats Using Modern Micro‐CT Scan‐Based Segmentation Techniques

Backer

Vos

Burnell

et al. 2009

The Anatomical Record

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Animal models are being used extensively in pre-clinical and safety assessment studies to assess the effectiveness and safety of new chemical entities and delivery systems. Although never entirely replacing the need for animal testing, the use of computer simulations could eventually reduce the amount of animals needed for research purposes and refine the data acquired from the animal studies. Computational fluid dynamics is a powerful tool that makes it possible to simulate flow and particle behavior in animal or patient-specific respiratory models, for purposes of inhaled delivery. This tool requires an accurate representation of the respiratory system, respiration and dose delivery attributes. The aim of this study is to develop a representative airway model of the Sprague-Dawley rat using static and dynamic micro-CT scans. The entire respiratory tract was modeled, from the snout and nares down to the central airways at the point where no distinction could be made between intraluminal air and the surrounding tissue. For the selection of the representative model, variables such as upper airway movement, segmentation length, airway volume and size are taken into account. Dynamic scans of the nostril region were used to illustrate the characteristic morphology of this region in anaesthetized animals. It could be concluded from this study that it was possible to construct a highly detailed representative model of a Sprague-Dawley rat based on imaging modalities such as micro-CT scans.

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Bronchial circulation angiogenesis in the rat quantified with SPECT and micro-CT

Abstract: Introduction-As pulmonary artery obstruction results in proliferation of the bronchial circulation in a variety of species, we investigated this angiogenic response using single photon emission computed tomography (SPECT) and micro-CT.

Cited by 18 publications

References 36 publications

Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs

Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs

Difference in intraosseous blood vessel volume and number in osteoporotic model mice induced by spinal cord injury and sciatic nerve resection

Study of the Variability in Upper and Lower Airway Morphology in Sprague–Dawley Rats Using Modern Micro‐CT Scan‐Based Segmentation Techniques

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