Broad-spectrum antimicrobial activity of volatile organic compounds from endophytic <i>Pseudomonas putida</i> BP25 against diverse plant pathogens

Agisha, V. N.; Kumar, Aundy; Eapen, Santhosh J.; Sheoran, Neelam; Suseelabhai, Rajamma

doi:10.1080/09583157.2019.1657067

Cited by 62 publications

(33 citation statements)

References 54 publications

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“…2,5-Dimethylpyrazine (4), attenuated (> 30%) the growth of all plant pathogens at the dosage of 7.5 mg/Petri. This VOC may be produced by various soil bacteria and has been shown to inhibit the growth of several diverse plant pathogens (Haidar et al, 2016;Agisha et al, 2019). The broad distribution and activity indicate a possible role of this compound under environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Volatile Organic Compounds From Lysobacter capsici AZ78 as Potential Candidates for Biological Control of Soilborne Plant Pathogens

Vlassi

Nesler²,

Perazzolli

et al. 2020

Front. Microbiol.

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The genus Lysobacter includes several bacterial species which show potential for being used in biological control of plant diseases. It was shown recently that several Lysobacter type strains produce volatile organic compounds (VOCs) which controlled the growth of Phytophthora infestans in vitro when the bacteria were grown on a protein rich medium. In the present study, Lysobacter capsici AZ78 (AZ78) has been tested for its potential to produce VOCs that may contribute to the bioactivity against soilborne plant pathogens. To this end, split Petri dish assays of bacterial cultures have been combined with GC-MS measurements with the aim to reveal the identity of the VOCs which inhibit the growth of Pythium ultimum Rhizoctonia solani, and Sclerotinia minor. While AZ78 completely suppressed the growth of P. ultimum and S. minor, the growth of R. solani was still reduced significantly. The GC-MS analysis revealed 22 VOCs to be produced by AZ78, the majority of which were (putatively) identified as mono-and dialkylated methoxypyrazines. Based on additional cultivation and GC-MS experiments, 2,5-dimethylpyrazine, 2-ethyl-3-methoxypyrazine and 2-isopropyl-3-methoxypyrazine were selected as presumable bioactive compounds. Further bioassays employing indirect exposure to standard solutions (1-10 mg per Petri dish) of the synthetic compounds via the gas phase, revealed that each of these pyrazines was able to suppress the growth of the pathogens under investigation. The results of this study highlight the possible future implementation of pyrazine derivatives in the control of soilborne plant diseases and further support the biocontrol potential of L. capsici AZ78.

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Section: Discussionmentioning

confidence: 99%

Volatile Organic Compounds From Lysobacter capsici AZ78 as Potential Candidates for Biological Control of Soilborne Plant Pathogens

Vlassi

Nesler²,

Perazzolli

et al. 2020

Front. Microbiol.

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“…Of the VC identified in these bacterial isolates, S-methyl thiobutyrate, butyl isovalerate, ethyl 3,3-dimethylacrylate, and 1-methoxy-4-methylbenzene showed more than 90% nematicidal activity against M. incognita ( Xu et al, 2015 ). VCs from Pseudomonas putida BP25 were tested against Radopholus similis and showed more than 92% mortality ( Agisha et al 2019 ; Sheoran et al, 2015 ). Many VCs were identified such as 1-undecene; disulfide dimethyl; pyrazine, methyl-pyrazine, 2,5-dimethyl-; isoamyl alcohol; pyrazine, and methyl-; dimethyl trisulfide.…”

Section: Bacterial Volatile Compounds Against Plant-parasitic Nematodmentioning

confidence: 99%

Volatile compounds as potential bio-fumigants against plant-parasitic nematodes – a mini review

Bui

Desaeger

2021

Journal of Nematology

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Soil fumigation remains the standard practice to manage soilborne pathogens such as plant-parasitic nematodes, bacteria, and fungi, especially in high-value crops. However, increasing regulatory pressure due to the inherent and broad-spectrum toxicity and negative environmental impact of chemical soil fumigants, its negative effect on overall soil health, and increasing demand for organic produce, has created a growing interest in biological fumigants. Many plants and microorganisms emit volatile compounds, which can potentially be used as bio-fumigants. In this mini-review, we summarize the current status of nematology studies focused on the development of volatile compounds emitted from plants and microorganisms as fumigants to control plant-parasitic nematodes. The gap of knowledge and challenges of studying volatile compounds are also addressed.

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“…Furthermore, due to their highly diffusible capacity, microbial VOCs are considered ideal for biocontrol approaches because they do not require the contact between antagonist and pathogen to perform their activity ( Contarino et al, 2019 ). Previous studies have shown VOCs potential from Pseudomonas ( Agisha et al, 2019 ), Bacillus ( Gao et al, 2018 ), and Alcaligenes ( Gong et al, 2019 ) to inhibit the growth of different pathogens, such as fungal, oomycete, and nematode, as well as disease suppression caused by them. Besides VOCs, both siderophores and lytic enzymes could be also involved in the inhibition mechanism of Pss growth.…”

Section: Discussionmentioning

confidence: 99%

Screening the Olive Tree Phyllosphere: Search and Find Potential Antagonists Against Pseudomonas savastanoi pv. savastanoi

et al. 2020

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Olive knot (OK) is a widespread bacterial disease, caused by Pseudomonas savastanoi pv. savastanoi ( Pss ), which currently has not effective control methods. The use of naturally occurring microbial antagonists, such as bacteria, as biocontrol agents could be a strategy to manage this disease. The objective of this work was to select bacteria from olive tree phyllosphere able to antagonize Pss using in vitro and in planta experiments. The elucidation of their modes of action and the potential relationship between antagonism and bacteria origin has been investigated, as well. To this end, 60 bacterial isolates obtained from the surface and inner tissues of different organs (leaves, twigs, and knots), from two olive cultivars of varying susceptibilities to OK, were screened for their in vitro antagonistic effect against Pss . A total of 27 bacterial strains were able to significantly inhibit Pss growth, being this effect linked to bacteria origin. Strains from OK-susceptible cultivar and colonizing the surface of plant tissues showed the strongest antagonistic potential. The antagonistic activity was potentially due to the production of volatile compounds, siderophores and lytic enzymes. Bacillus amyloliquefaciens P41 was the most effective antagonistic strain and their capacity to control OK disease was subsequently assayed using in planta experiments. This strain significantly reduces OK disease severity (43.7%), knots weight (55.4%) and population size of Pss (26.8%), while increasing the shoot dry weight (55.0%) and root water content (39.6%) of Pss -infected olive plantlets. Bacterial isolates characterized in this study, in particular B. amyloliquefaciens P41, may be considered as promising biocontrol candidates for controlling OK disease.

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Broad-spectrum antimicrobial activity of volatile organic compounds from endophytic Pseudomonas putida BP25 against diverse plant pathogens

Cited by 62 publications

References 54 publications

Volatile Organic Compounds From Lysobacter capsici AZ78 as Potential Candidates for Biological Control of Soilborne Plant Pathogens

Volatile Organic Compounds From Lysobacter capsici AZ78 as Potential Candidates for Biological Control of Soilborne Plant Pathogens

Volatile compounds as potential bio-fumigants against plant-parasitic nematodes – a mini review

Screening the Olive Tree Phyllosphere: Search and Find Potential Antagonists Against Pseudomonas savastanoi pv. savastanoi

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