Brillouin spectroscopy of biorelevant fluids in relation to viscosity and solute concentration

Adichtchev, S. V.; Karpegina, Yu. A.; Okotrub, Konstantin A.; Суровцева, М. А.; Zykova, V. A.; Surovtsev, N. V.

doi:10.1103/physreve.99.062410

Cited by 23 publications

(30 citation statements)

References 27 publications

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“…The former has previously been established through the use of pore-elastic models (30,31); however, recent work suggests that the contribution of water is far more complex, with cell mechanics heavily affected by water (32)(33)(34), in particular osmotic-induced volume change affects cell stiffness (32) and deformability (34), fluid flow through cell-cell gap junctions induces mechanical pattern formation (33), and cell migration in confinement is driven by cell volume regulation (35). The contribution of water to the Brillouin spectrum is still a subject of controversy with some reports that in highly hydrated fluids, simulating some aspects of the cell cytoplasm, the frequency shift of the Brillouin peak is determined by modes generated in the water phase (36,37).…”

Section: Introductionmentioning

confidence: 99%

Viscoelastic properties of biopolymer hydrogels determined by Brillouin spectroscopy: A probe of tissue micromechanics

et al. 2020

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Many problems in mechanobiology urgently require characterization of the micromechanical properties of cells and tissues. Brillouin light scattering has been proposed as an emerging optical elastography technique to meet this need. However, the information contained in the Brillouin spectrum is still a matter of debate because of fundamental problems in understanding the role of water in biomechanics and in relating the Brillouin data to low-frequency macroscopic mechanical parameters. Here, we investigate this question using gelatin as a model system in which the macroscopic physical properties can be manipulated to mimic all the relevant biological states of matter, ranging from the liquid to the gel and the glassy phase. We demonstrate that Brillouin spectroscopy is able to reveal both the elastic and viscous properties of biopolymers that are central to the structure and function of biological tissues.

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Section: Introductionmentioning

confidence: 99%

Viscoelastic properties of biopolymer hydrogels determined by Brillouin spectroscopy: A probe of tissue micromechanics

et al. 2020

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“…This response can be integrated with the refractive index to extract the Brillouin elastic contrast (

), which can reveal changes in stiffness of the sample examined. Variations in refractive index and mass density in tissues are usually small, leading to negligible changes in Brillouin shifts ( 8 ). For this reason, we interpret changes in the Brillouin shifts as the signature of drastic changes in cell wall stiffness.…”

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confidence: 99%

THESEUS1 modulates cell wall stiffness and abscisic acid production in Arabidopsis thaliana

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Schulz

Engelsdorf

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Plant cells can be distinguished from animal cells by their cell walls and high-turgor pressure. Although changes in turgor and the stiffness of cell walls seem coordinated, we know little about the mechanism responsible for coordination. Evidence has accumulated that plants, like yeast, have a dedicated cell wall integrity maintenance mechanism. It monitors the functional integrity of the wall and maintains integrity through adaptive responses induced by cell wall damage arising during growth, development, and interactions with the environment. These adaptive responses include osmosensitive induction of phytohormone production, defense responses, as well as changes in cell wall composition and structure. Here, we investigate how the cell wall integrity maintenance mechanism coordinates changes in cell wall stiffness and turgor in Arabidopsis thaliana. We show that the production of abscisic acid (ABA), the phytohormone-modulating turgor pressure, and responses to drought depend on the presence of a functional cell wall. We find that the cell wall integrity sensor THESEUS1 modulates mechanical properties of walls, turgor loss point, ABA biosynthesis, and ABA-controlled processes. We identify RECEPTOR-LIKE PROTEIN 12 as a component of cell wall integrity maintenance–controlling, cell wall damage–induced jasmonic acid (JA) production. We propose that THE1 is responsible for coordinating changes in turgor pressure and cell wall stiffness.

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“…56,57 This approximation also works for other solute molecules, not exclusively for polymers. 58 Yet, the general temperature dependence of the longitudinal modulus is not covered by a binary mixture model. We thus use a simplified binary mixture model and approximate the general temperature dependence of M by…”

Section: Temperature-dependent Micromechanics Of Pnipaam Microgelsmentioning

confidence: 99%

PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers

et al. 2022

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Brillouin spectroscopy of biorelevant fluids in relation to viscosity and solute concentration

Cited by 23 publications

References 27 publications

Viscoelastic properties of biopolymer hydrogels determined by Brillouin spectroscopy: A probe of tissue micromechanics

Viscoelastic properties of biopolymer hydrogels determined by Brillouin spectroscopy: A probe of tissue micromechanics

THESEUS1 modulates cell wall stiffness and abscisic acid production in Arabidopsis thaliana

PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers

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