Bridging the gap: Super-resolution microscopy of epithelial cell junctions

Bartle, Emily I.; Rao, Tejeshwar C.; Urner, Tara; Mattheyses, Alexa L.

doi:10.1080/21688370.2017.1404189

Cited by 9 publications

(12 citation statements)

References 113 publications

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“…The tight junctions and vascular endothelial cells are important components of the blood labyrinth barrier as well as ion channels [ 30 , 53 – 57 ]. Our cluster analysis of the RNA transcriptome data from both pigs and mice showed that the tight junctions were significantly different in the stria vascularis of these two species.…”

Section: Discussionmentioning

confidence: 99%

Transcript Profiles of Stria Vascularis in Models of Waardenburg Syndrome

et al. 2020

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Background. Waardenburg syndrome is an uncommon genetic condition characterized by at least some degree of congenital hearing loss and pigmentation deficiencies. However, the genetic pathway affecting the development of stria vascularis is not fully illustrated. Methods. The transcript profile of stria vascularis of Waardenburg syndrome was studied using Mitf-M mutant pig and mice models. Therefore, GO analysis was performed to identify the differential gene expression caused by Mitf-M mutation. Results. There were 113 genes in tyrosine metabolism, melanin formation, and ion transportations showed significant changes in pig models and 191 genes in mice models. In addition, there were some spice’s specific gene changes in the stria vascularis in the mouse and porcine models. The expression of tight junction-associated genes, including Cadm1, Cldn11, Pcdh1, Pcdh19, and Cdh24 genes, were significantly higher in porcine models compared to mouse models. Vascular-related and ion channel-related genes in the stria vascularis were also shown significantly difference between the two species. The expression of Col2a1, Col3a1, Col11a1, and Col11a2 genes were higher, and the expression of Col8a2, Cd34, and Ncam genes were lower in the porcine models compared to mouse models. Conclusions. Our data suggests that there is a significant difference on the gene expression and function between these two models.

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Section: Discussionmentioning

confidence: 99%

Transcript Profiles of Stria Vascularis in Models of Waardenburg Syndrome

et al. 2020

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“…This study does not determine the precise subcellular protein localization in human endoneurial microvascular endothelial cells. Immunogold electron microscopy and super resolution microscopy provide avenues to more precisely determine the in situ subcellular localization of specific molecules restricted to the human BNB, particularly components of the restrictive intercellular junctional complex such as CLDN4, or determine the luminal or abluminal localization of transporters such as SLC1A1 prior to functional studies. This is an important future direction.…”

Section: Discussionmentioning

confidence: 99%

“…Immunogold electron microscopy and super resolution microscopy [46][47][48][49] ORCID Eroboghene E. Ubogu https://orcid.org/0000-0002-8307-9994…”

Section: Discussionmentioning

confidence: 99%

In situ molecular characterization of endoneurial microvessels that form the blood‐nerve barrier in normal human adult peripheral nerves

Ouyang

Dong

Ubogu

2019

J Peripheral Nervous Sys

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The blood‐nerve barrier (BNB) formed by tight junction‐forming endoneurial microvessels located in the innermost compartment of peripheral nerves, and the perineurium serve to maintain the internal microenvironment required for normal signal transduction. The specific molecular components that define the normal adult human BNB are not fully known. Guided by data derived from the adult human BNB transcriptome, we evaluated the in situ expression of 25 junctional complex, transporter, cell membrane, and cytoskeletal proteins in four histologically normal adult sural nerves by indirect fluorescent immunohistochemistry to determine proteins specifically expressed by restrictive endoneurial microvascular endothelium. Using Ulex Europaeus Agglutinin‐1 expression to detect endothelial cells, we ascertained that the selected proteins were uniformly expressed in ≥90% of endoneurial microvessels. P‐glycoprotein (also known as adenosine triphosphate‐binding cassette subfamily B member 1) and solute carrier family 1 member 1 demonstrated restricted expression by endoneurial endothelium only, with classic tight junction protein claudin‐5 also expressed on fenestrated epineurial macrovessels, and vascular‐specific adherens junction protein cadherin‐5 also expressed by the perineurium. The expression profiles of the selected proteins provide significant insight into the molecular composition of normal adult peripheral nerves. Further work is required to elucidate the human adult BNB molecular signature in order to better understand its development and devise strategies to restore function in peripheral neuropathies.

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“…The tight junctions and vascular endothelial cells are important components of the blood labyrinth barrier as well as ion channels [42][43][44][45][46] . Our cluster analysis of the RNA transcriptome data from both pigs and mice, showed that the tight junctions were significantly different in the stria vascularis of these two species.…”

Section: Discussionmentioning

confidence: 99%

Transcript profiles of stria vascularis in models of Waardenburg syndrome

Chen

Wang

Chen

et al. 2019

Preprint

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Background: Waardenburg syndrome is a common syndromic hereditary deafness disease caused by stria vascularis dysfunction. However, the genetic pathway affecting stria vascularis development is still not clear. In this paper, the transcript profile of stria vascularis of Waardenburg syndrome was studied using Mitf-M mutant pigs and mice models. GO analysis was performed to identify the differential gene expression caused by Mitf-M mutation. Results: There were over than one hundred genes mainly found in tyrosine metabolism, melanin formation and ion transportations showed significant changes in both models. In addition, there were some spiced specific gene changes in the stria vascularis in the mouse and porcine models. The expression of tight junction-associated genes, including Cadm1, Cldn11, Pcdh1, Pcdh19 and Cdh24 genes, were significantly higher in porcine models compared to mouse models. Vascular-related and ion channel-related genes in the stria vascularis were also shown significantly difference between the two species. The expression of Col2a1, Col3a1, Col11a1 and Col11a2 genes were higher and the expression of Col8a2, Cd34 and Ncam genes were lower in the porcine model compared to mouse model. In both models, Trpm1, Kcnj13, and Slc45a2 genes were both affected by the Mitf-M mutation. In the pig models, the expression of Kcnn1, Clcn2 and Trpm4 genes were higher than the mouse model; whereas the expression of Trpm7, Kcnq1 and Kcnj8 genes were higher in the mouse models than the pig models. However, there was no significant difference in the morphology of the stria vascularis between these two models. Conclusions: Our data suggests that there is a significant difference on the gene expression and function between these two models.

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Bridging the gap: Super-resolution microscopy of epithelial cell junctions

Cited by 9 publications

References 113 publications

Transcript Profiles of Stria Vascularis in Models of Waardenburg Syndrome

Transcript Profiles of Stria Vascularis in Models of Waardenburg Syndrome

In situ molecular characterization of endoneurial microvessels that form the blood‐nerve barrier in normal human adult peripheral nerves

Transcript profiles of stria vascularis in models of Waardenburg syndrome

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