Brevilin A enhances innate immunity and the resistance of oxidative stress in Caenorhabditis elegans via p38 MAPK pathway

Zhu, Xiao‐Feng; Liu, Fang; Wu, Qin-Yi; Li, Sanhua; Ruan, Guoyong; Yang, Jian‐Bo; Yu, Changyan; Jiang, Nian; Xiao, Yi; Liu, Yun

doi:10.1016/j.intimp.2022.109385

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…50 Zhu et al found that Brevilin A failed to confer resistance to P. aeruginosa 14 infection in nsy-1 and sek-1 mutants, suggesting that Brevilin A enhances the innate immunity of nematodes through the p38 MAPK pathway. 51 The lifespan analysis experiment results from the research of Negi et al showed that the mean lifespan of mutants with deletions of crucial genes in the JNK MAPK pathway, including jnk-1 and jkk-1 , did not exhibit significant changes under ursolic acid exposure, suggesting that the ursolic acid was mediating longevity in a JNK-1 dependent manner. 52 Previous studies on C. elegans have shown that SKN-1, activated by sek-1 , nsy-1 and pmk-1 , could synthesize ROS-resistant detoxifying enzymes, thereby assisting in the regulation of DAF-16.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Song,

Liu,

Xie

et al. 2024

Food Funct.

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Section: Discussionmentioning

confidence: 99%

Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Song,

Liu,

Xie

et al. 2024

Food Funct.

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“…TCM products have demonstrated anticancer effects through diverse pathways and mechanisms [7]. Brevilin A, a sesquiterpene lactone derived from C. minima, exhibits a spectrum of pharmacological activities encompassing anti-cancer [8,9], anti-oxidative [10], antiin ammatory [11,12], and immune-enhancing effects [13]. Previous studies have shown the potential of Brevilin A in combating various human malignancies, including lung cancer [14], nasopharyngeal carcinoma [15], multiple myeloma [16], gastric cancer [17], breast cancer [18], and prostate cancer [19].…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Prediction and validation of Brevilin A Therapeutic Effects in Lung Cancer

Wang,

Gao,

et al. 2024

Preprint

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Background Traditional Chinese medicine (TCM) has found widespread application in neoplasm treatment, yielding promising therapeutic candidates. Previous studies have revealed the anti-cancer properties of Brevilin A, a naturally occurring sesquiterpene lactone derived from Centipeda minima (L.) A.Br. (C. minima), a TCM herb, specifically against lung cancer. However, the underlying mechanisms of its effects remain elusive. This study employs network pharmacology and experimental analyses to unravel the molecular mechanisms of Brevilin A in lung cancer. Methods The Batman-TCM, Swiss Target Prediction, Pharmmapper, SuperPred, and BindingDB databases were screened to identify Brevilin A targets. Lung cancer-related targets were sourced from GEO, Genecards, OMIM, TTD, and Drugbank databases. Utilizing Cytoscape software, a protein-protein interaction (PPI) network was established. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), set enrichment analysis (GSEA), and gene-pathway correlation analysis were conducted using R software. To validate network pharmacology results, molecular docking, molecular dynamics simulations, and in vitro experiments were performed. Results We identified 599 Brevilin A-associated targets and 3864 lung cancer-related targets, with 155 overlapping genes considered as candidate targets for Brevilin A against lung cancer. The PPI network highlighted STAT3, TNF, HIF1A, PTEN, ESR1, and MTOR as potential therapeutic targets. GO and KEGG analyses revealed 2893 enriched GO terms and 157 enriched KEGG pathways, including the PI3K-Akt signaling pathway, FoxO signaling pathway, and HIF-1 signaling pathway. GSEA demonstrated a close association between hub genes and lung cancer. Gene-pathway correlation analysis indicated significant associations between hub genes and the cellular response to hypoxia pathway. Molecular docking and dynamics simulations confirmed Brevilin A's interaction with PTEN and HIF1A, respectively. In vitro experiments demonstrated Brevilin A-induced dose- and time-dependent cell death in A549 cells. Notably, Brevilin A treatment significantly reduced HIF-1α mRNA expression while increasing PTEN mRNA levels. Conclusions This study elucidates the intricate molecular mechanisms responsible for the therapeutic effects of Brevilin A on lung cancer, providing a molecular foundation for future exploration of Brevilin A's therapeutic potential.

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“…Brevilin A (BA) possesses anti-inflammatory properties [20,21], acts as an antioxidant [22,23], enhances skin inflammation [24], and exhibits various other biological functions. Although BA exhibits remarkable anti-inflammatory properties, it struggles with limited water solubility and low bioavailability.…”

Section: Introductionmentioning

confidence: 99%

“…Notably, it exhibits potential as a drug carrier [30]. Burns are frequently treated with CO in traditional medicine, and research has demonstrated that CO possesses antioxidant, protective, anti-inflammatory, and antibacterial properties [24,31,32].…”

Section: Introductionmentioning

confidence: 99%

Dual-Delivery Temperature-Sensitive Hydrogel with Antimicrobial and Anti-Inflammatory Brevilin A and Nitric Oxide for Wound Healing in Bacterial Infection

Ruan,

Pan,

Ran

et al. 2024

Gels

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Bacterial infections impede the wound healing process and can trigger local or systemic inflammatory responses. Therefore, there is an urgent need to develop a dressing with antimicrobial and anti-inflammatory properties to promote the healing of infected wounds. In this study, BA/COs/NO-PL/AL hydrogels were obtained by adding brevilin A (BA) camellia oil (CO) submicron emulsion and nitric oxide (NO) to hydrogels consisting of sodium alginate (AL) and Pluronic F127 (PL). The hydrogels were characterized through dynamic viscosity analysis, differential scanning calorimetry, and rheology. They were evaluated through anti-inflammatory, antimicrobial, and wound healing property analyses. The results showed that BA/COs/NO-PL/AL hydrogels were thermo-responsive and had good ex vivo and in vivo anti-inflammatory activity, and they also exhibited strong antimicrobial activity against methicillin-resistant Staphylococcus aureus Pseudomonas aeruginosa (MRPA) and methicillin-resistant Staphylococcus aureus (MRSA). They were able to effectively promote healing of the infected wound model and reduce inflammation and bacterial burden. H&E and Masson’s staining showed that BA/COs/NO-PL/AL hydrogels promoted normal epithelial formation and collagen deposition. In conclusion, BA/COs/NO-PL/AL hydrogels are promising candidates for promoting the healing of infected wounds.

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Brevilin A enhances innate immunity and the resistance of oxidative stress in Caenorhabditis elegans via p38 MAPK pathway

Cited by 10 publications

References 35 publications

Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Mechanistic Prediction and validation of Brevilin A Therapeutic Effects in Lung Cancer

Dual-Delivery Temperature-Sensitive Hydrogel with Antimicrobial and Anti-Inflammatory Brevilin A and Nitric Oxide for Wound Healing in Bacterial Infection

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