Breakage of lysozyme crystals due to compressive stresses during cake filtration

Cornehl, Bianca; Overbeck, Achim; Schwab, Annika; Büser, Jan-Philipp; Kwade, Arno; Nirschl, Hermann

doi:10.1016/j.ces.2014.02.016

Cited by 22 publications

(18 citation statements)

References 11 publications

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“…At higher revolutions than 3000 rpm no further reduction of the particle size is visible. Because 3000 rpm correspond approximately 0.9 × 10 5 Pa, those results are in good agreement with the results obtained by Cornehl et al (2014) with the pressure filter cell.…”

Section: Crystal Breakagesupporting

confidence: 89%

“…Due to its antibacterial effect it is often used in the food industries to increase the shelf life of cheese. Hen-egg white lysozyme is available on a large scale and has been well characterized (Durbin and Feher, 1986;Howard et al, 1988;Koizumi et al, 2004;Hekmat et al, 2007;Liu et al, 2010;Zamiri and De, 2010;Eichholz et al, 2011;Cornehl et al, 2013Cornehl et al, , 2014Liu et al, 2016). Because many properties have already been investigated and its good and inexpensive availability, lysozyme is a good model protein for this study.…”

Section: Lysozymementioning

confidence: 99%

“…The compressive breakage behavior of lysozyme crystals was studied by Cornehl et al (2014). With a compression-permeability cell and a pressure filter cell Cornehl et al investigated the breakage for different pressures, the kinetics of the crystal breakage and also the changes in filter cake resistance and porosity.…”

Section: Mechanical Stability Of Lysozymementioning

confidence: 99%

“…The compression can be explained by restructuring of the filter cake and also by crystal breakage. Cornehl et al (2014) demonstrated that even low pressure differences of 0.5 × 10 5 Pa can break lysozyme crystals.…”

Section: Filter Cake Resistancementioning

confidence: 99%

“…Cornehl et al found that even low mechanical stress can lead to crystal breakage or crystal abrasion. The smaller fragments lead to a decrease of the filter cake porosity and thus making the solid liquid separation a challenging task (Cornehl et al, 2013(Cornehl et al, , 2014. Therapeutic proteins, especially monoclonal antibodies are expensive products.…”

Section: Introductionmentioning

confidence: 99%

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Determination of filtration and consolidation properties of protein crystal suspensions using analytical photocentrifuges with low volume samples

Radel

Funck

Nguyen

et al. 2019

Chemical Engineering Science

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Proteins and in particular pharmaceutical proteins like monoclonal antibodies are expensive products. Protein crystallization is an interesting alternative process step to the common purification and formulation of such proteins. The solid-liquid separation of proteins however, is a challenging task because the proteins are sensitive to mechanical stress, which could lead to crystal breakage and often are only available in small amounts for research and development. In this study a newly developed filtration cell for the LUMiSizer R centrifuge is used to analyze the filtration behavior with low volume samples. Isometric and needle shaped lysozyme crystals serve as model protein crystals. The needle shaped crystals showed about twice as high compressibility and much higher cake resistance than the isometric lysozyme crystals. After the filtration at maximum pressure in the centrifuge the mean particle diameter decreased compared to the unstressed median diameter. With the filtration cell the cake height and filter cake compression can be monitored in situ and the filter cake resistance and solids volume fraction can be calculated with one experiment using 200 to 300 µl sample.

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Section: Crystal Breakagesupporting

confidence: 89%

Section: Lysozymementioning

confidence: 99%

Section: Mechanical Stability Of Lysozymementioning

confidence: 99%

Section: Filter Cake Resistancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of filtration and consolidation properties of protein crystal suspensions using analytical photocentrifuges with low volume samples

Radel

Funck

Nguyen

et al. 2019

Chemical Engineering Science

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Calculation of the flux density function for protein crystals from small scale settling and filtration experiments

2021

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Development and engineering of protein crystals regarding mechanical stability and crystallizability occurs on a small scale. Later in the process chain of industrial production however, filtration properties are important to separate the crystals from mother liquor. Many protein crystals are sensitive to mechanical stress which is why it is important to know the filtration behavior early on. In this study, we analyze settling and filtration behavior of isometric, rod-like and needle shaped lysozyme and rod-like alcohol dehydrogenase (ADH) crystals on a small scale using an optical analytical centrifuge. Needle shaped lysozyme and rod-like ADH crystals show compressible material behavior. With the results from settling and filtration experiments, the flux density function is calculated and modeled which can be used to describe the whole settling and permeation process in dependency of the solids volume fraction. This is also an issue for simulations of industrial processes.

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Preparative Protein Crystallization

2019

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Preparative protein crystallization can possibly replace one or more chromatographic steps in downstream processing. The development of such crystallization processes is demanding: first, promising principal crystallization conditions must be identified; second, details about the process must be defined; and third, the crystals have to be separated from the mother liquor without putting harm of their crystalline integrity. State‐of‐the‐art about these three steps is developing fast by (i) employing new screening methods which are based on fundamental understanding of the interaction of the protein molecules, (ii) application of existing concepts of technical bulk crystallization of small molecules to preparative protein crystallization, and (iii) making available specific gentle separation machinery.

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Breakage of lysozyme crystals due to compressive stresses during cake filtration

Cited by 22 publications

References 11 publications

Determination of filtration and consolidation properties of protein crystal suspensions using analytical photocentrifuges with low volume samples

Determination of filtration and consolidation properties of protein crystal suspensions using analytical photocentrifuges with low volume samples

Calculation of the flux density function for protein crystals from small scale settling and filtration experiments

Preparative Protein Crystallization

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