Brain morphology and immunohistochemical localization of the gonadotropin-releasing hormone in the bluefin tuna, Thunnus thynnus

Palmieri, G; Acone, F; Desantis, Salvatore; Corriero, Aldo; Ventriglia, Gianluca; Addis, Pierantonio; Genovese, Silvia; Aprea, A; Spedicato, D; Losurdo, Michele; Deflorio, M.; Summa, Aldo Di; Metrio, G. De

doi:10.4081/1182

Cited by 10 publications

(7 citation statements)

References 47 publications

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“…Three types of neuronal cells, including large, medium and small-sized neurons, were sbGnRH-positive in the immunohistochemistry and immunofluorescence assay (Figs 4 and 5), indicating that these cells are involved in the production of the sbGnRH-like peptide in this fish. Several previous works also showed the occurrence of sbGnRH-ir neuronal cells in the preopticus-periventricularis nucleus of the brain in other fish species: Thunnus thynnus (Palmieri et al 2008), Paralichthys olivaceus (Pham et al 2007) and Scomber japonicus (Selvaraj et al 2009). However, these studies did not identify the neuronal types, so this is the first report that has shown that the large, medium and small-sized neurons in the preopticus-periventricularis nucleus can produce the GnRH-like peptide.…”

Section: Discussionmentioning

confidence: 75%

Distribution and changes in the sbGnRH system in Rastrelliger brachysoma males during the breeding season

Senarat

Kettratad

Jiraungkoorskul

et al. 2021

scimar

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Rastrelliger brachysoma is a mariculture candidate species, but reproduction in captive fish has been problematic. This report examines the difference in the HPG axis, the neuroendocrine system and the development of reproductive tissues between captive vs. wild male R. brachysoma. The gonadosomatic index (GSI) of sexually mature male wild R. brachysoma was 1.12±0.34 and 1.94±0.26 during the non-breeding and breeding seasons, respectively. Captive R. brachysoma had a GSI of 1.88±0.17. All wild R. brachysoma were in the late spermatogenic stage irrespective of seasons. Immunostaining results showed that sbGnRH-immunoreactive neurons were distributed in three areas of the brain, namely the nucleus periventricularis, nucleus preopticus and nucleus lateralis tuberis. Follicle stimulating hormone and luteinizing hormone immunoreactivities were also observed in the pituitary gland. The levels of brain sbGnRH and GtH mRNA were not significantly different between the non-breeding and breeding seasons, but captive fish displayed (times or percent difference) lower mRNA levels than wild fish. These results suggest that these hormones control the testicular development in R. brachysoma and that the impaired reproduction in captivity may be due to their relative lower expression levels of follicle stimulating hormone and luteinizing hormone genes.

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Section: Discussionmentioning

confidence: 75%

Distribution and changes in the sbGnRH system in Rastrelliger brachysoma males during the breeding season

Senarat

Kettratad

Jiraungkoorskul

et al. 2021

scimar

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“…These results indicate that GnRH2 does not regulate GTH secretion directly, but GnRH2 can be a neuromodulator and influence GTH in other ways. Although GnRH2-ir cell bodies were detected in the dorsal part of the PO as in the case of bluefin tuna Thunnus thynnus (Palmieri et al, 2008), gnrh2-expressing neurons were not detected in this region by in situ hybridization. Because the localization of GnRH1-ir cell bodies and that of GnRH2-ir cell bodies in the PO were different, and GnRH3-ir cell bodies were not detected in the PO, the GnRH2-ir cell bodies detected in the dorsal part of the PO were not the result of a cross-reaction between GnRH1 and GnRH3.…”

Section: Discussionmentioning

confidence: 85%

Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus

Amano

Amiya

Okubo

et al. 2019

Fish Physiol Biochem

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The localization of gonadotropin-releasing hormone (GnRH) in the brain and pituitary of the self-fertilizing mangrove killifish Kryptolebias marmoratus was examined by immunohistochemistry and in situ hybridization to understand its neuroendocrine system. The genome assembly of K. marmoratus did not have any sequence encoding GnRH1, but sequences encoding GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH) were found. Therefore, GnRH1 was identified by in silico cloning. The deduced amino acid sequence of the K. marmoratus GnRH1 (mature peptide) was identical to that of the medaka GnRH. GnRH1 neurons were detected in the ventral part of the preoptic nucleus (PO) by immunohistochemistry and in situ hybridization and GnRH1-immunoreactive (ir) fibers were observed throughout the brain. GnRH1-ir fibers were in close contact with luteinizing hormone (LH)-ir cells in the pituitary using double immunohistochemistry. GnRH2 neurons were detected in the midbrain tegmentum by immunohistochemistry and in situ hybridization. Although GnRH2-ir fibers were observed throughout the brain, they were not detected in the pituitary. GnRH3 neurons were detected in the lateral part of the ventral telencephalic area by both methods. GnRH3-ir fibers were observed throughout the brain, and a few GnRH3-ir fibers were in close contact with LH-ir cells in the pituitary. These results indicate that GnRH1 and possibly GnRH3 are responsible for gonadal maturation through LH secretion and that all three forms of GnRH function as neurotransmitters or neuromodulators in the brain of K. marmoratus.

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“…A previous observation showed that all populations were positively reacted with anti-GnRH-like peptide antibody in R. brachysoma (Senarat et al, 2019), and at present it is unclear how the functions of these three neuronal populations are different. Since these three nuclei are important regions for GnRH production in R. brachysoma (Senarat et al, 2019) as well as other fishes (Peter et al, 1991;Palmieri et al, 2008), further investigation on these three types of neurons are required.…”

Section: Resultsmentioning

confidence: 99%

Immunoreactivity of estrogen receptor alpha in brain and ovary of the short mackerel Rastrelliger brachysoma (Bleeker, 1851)

Senarat

Kettratad

Kangwanrangsan

et al. 2019

APJMBB

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The reproductive physiology concerning the gonadotropin hormone (GTH) and its downstream target estrogen receptor alpha (ERα) is not well understood in the short mackerel Rastrelliger brachysoma, an economically important marine fish in Thailand. In this study, we tested the hypothesis that the expression of both GTH and ERα in the brain and ovary of R. brachysoma was as reported in other fish species. By applying immunohistochemical techniques, we identified the distribution of ERα-immunoreactive (ir) neurons in the brain and ovary of wild female R. brachysoma during the spawning season along with the distribution of GTHs-ir cells in the ovary. The nucleus lateralis tuberis in the diencephalon had a high number of ERα-ir neurons. In the mesencephalon, dense ERα-ir neuronal fibers were mainly found in the mesencephalic cells, stratum opticum, stratum fibrosum et griseum superficiale and stratum album centrale. Both the valvula and corpus cerebelli in the metencephalon contained ERα-ir neurons in granular and Purkinje cell layers as well as the molecular layer. The ERα-ir neurons were also observed in the medulla oblongata. In the ovary, weak ERα and moderate GTHs immunoreactivities were observed in follicular cells of oocytes in early and late vitellogenic stages. This information provides baseline data required to understand not only the activity of estrogen (E2) on the brain but also the regulatory mechanism of the hypothalamo-pituitary-ovarian axis of R. brachysoma.

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Brain morphology and immunohistochemical localization of the gonadotropin-releasing hormone in the bluefin tuna, Thunnus thynnus

Cited by 10 publications

References 47 publications

Distribution and changes in the sbGnRH system in Rastrelliger brachysoma males during the breeding season

Distribution and changes in the sbGnRH system in Rastrelliger brachysoma males during the breeding season

Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus

Immunoreactivity of estrogen receptor alpha in brain and ovary of the short mackerel Rastrelliger brachysoma (Bleeker, 1851)

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