Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion

Zou, Song-Song; Zou, Qing-Cui; Xiong, Wenjing; Cui, Ning-Yi; Wang, Ke; Liu, Haoxuan; Lou, Wen-Juan; Higazy, Doaa; Zhang, Yage; Cui, Min

doi:10.3389/fcimb.2021.701820

Cited by 12 publications

(15 citation statements)

References 39 publications

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“…Studies on the interaction between human brain endothelial cells and Flaviviruses have demonstrated CNS invasion by direct infection of these cells and suggest that the BBB is a key gateway for arbovirus brain infections. For instance, JEV and ZIKV are able to infect cerebral endothelial cells and cross the BBB [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity

Constant

Maarifi

Barthélémy

et al. 2023

Emerging Microbes & Infections

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Usutu (USUV) and West Nile (WNV) viruses are two closely related Flavivirus belonging to Japanese encephalitis virus serogroup. Evidence of increased circulation of these two arboviruses now exist in Europe. Neurological disorders are reported in humans mainly for WNV, despite the fact that the interaction and effects of viral infections on the neurovasculature are poorly described, notably for USUV. Using a human in vitro blood–brain barrier (BBB) and a mouse model, this study characterizes and compares the cerebral endothelial cell permissiveness, innate immunity and inflammatory responses and immune cell recruitment during infection by USUV and WNV. Both viruses are able to infect and cross the human BBB but with different consequences. We observed that WNV infects BBB cells resulting in significant endothelium impairment, potent neuroinflammation and immune cell recruitment, in agreement with previous studies. USUV, despite being able to infect BBB cells with higher replication rate than WNV, does not strongly affect endothelium integrity. Importantly, USUV also induces neuroinflammation, immune cell recruitment such as T lymphocytes, monocytes and dendritic cells (DCs) and was able to infect dendritic cells (DCs) more efficiently compared to WNV, with greater propensity for BBB recruitment. DCs may have differential roles for neuroinvasion of the two related viruses.

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Section: Introductionmentioning

confidence: 99%

Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity

Constant

Maarifi

Barthélémy

et al. 2023

Emerging Microbes & Infections

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“…In many flavivirus infections, especially DENV infection, a hallmark of viral pathogenesis leading to severe disease is alterations in the barrier function of the endothelium, resulting in transient vascular leak and hypotension that can lead to shock and potentially death [2, [54][55][56]. DENV, WNV, JEV, and ZIKV can alter the integrity of TJ/AJ proteins at the blood-brain and placental barriers [23,[57][58][59][60][61][62][63][64][65][66]. The altered expression and/or distribution of IJC proteins such as VE-cadherin, ZO-1, and β-catenin has been described in in vitro assays with epithelial and endothelial cells after DENV or other flaviviral infections [59][60][61][62][63][64][65][67][68][69][70][71], stimulation of human ECs with soluble factors circulating in sera from dengue patients [72][73][74] or secreted from DENV-infected cells [18,75], as well as in DENV and ZIKV NS1-stimulated umbilical vein ECs, ex vivo human chorionic explants, and dermal ECs [10,23,39,40,58,76,77].…”

Section: Discussionmentioning

confidence: 99%

“…DENV, WNV, JEV, and ZIKV can alter the integrity of TJ/AJ proteins at the blood-brain and placental barriers [23,[57][58][59][60][61][62][63][64][65][66]. The altered expression and/or distribution of IJC proteins such as VE-cadherin, ZO-1, and β-catenin has been described in in vitro assays with epithelial and endothelial cells after DENV or other flaviviral infections [59][60][61][62][63][64][65][67][68][69][70][71], stimulation of human ECs with soluble factors circulating in sera from dengue patients [72][73][74] or secreted from DENV-infected cells [18,75], as well as in DENV and ZIKV NS1-stimulated umbilical vein ECs, ex vivo human chorionic explants, and dermal ECs [10,23,39,40,58,76,77]. Importantly, IJC proteins have been found to circulate in the serum of dengue patients undergoing severe plasma leakage, and alterations of the IJC have been proposed as potential biomarkers for disease prognosis [15,22,78].…”

Section: Discussionmentioning

confidence: 99%

Flavivirus NS1 Triggers Tissue-Specific Disassembly of Intercellular Junctions Leading to Barrier Dysfunction and Vascular Leak in a GSK-3β-Dependent Manner

et al. 2022

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The flavivirus nonstructural protein 1 (NS1) is secreted from infected cells and contributes to endothelial barrier dysfunction and vascular leak in a tissue-dependent manner. This phenomenon occurs in part via disruption of the endothelial glycocalyx layer (EGL) lining the endothelium. Additionally, we and others have shown that soluble DENV NS1 induces disassembly of intercellular junctions (IJCs), a group of cellular proteins critical for maintaining endothelial homeostasis and regulating vascular permeability; however, the specific mechanisms by which NS1 mediates IJC disruption remain unclear. Here, we investigated the relative contribution of five flavivirus NS1 proteins, from dengue (DENV), Zika (ZIKV), West Nile (WNV), Japanese encephalitis (JEV), and yellow fever (YFV) viruses, to the expression and localization of the intercellular junction proteins β-catenin and VE-cadherin in endothelial cells from human umbilical vein and brain tissues. We found that flavivirus NS1 induced the mislocalization of β-catenin and VE-cadherin in a tissue-dependent manner, reflecting flavivirus disease tropism. Mechanistically, we observed that NS1 treatment of cells triggered internalization of VE-cadherin, likely via clathrin-mediated endocytosis, and phosphorylation of β-catenin, part of a canonical IJC remodeling pathway during breakdown of endothelial barriers that activates glycogen synthase kinase-3β (GSK-3β). Supporting this model, we found that a chemical inhibitor of GSK-3β reduced both NS1-induced permeability of human umbilical vein and brain microvascular endothelial cell monolayers in vitro and vascular leakage in a mouse dorsal intradermal model. These findings provide insight into the molecular mechanisms regulating NS1-mediated endothelial dysfunction and identify GSK-3β as a potential therapeutic target for treatment of vascular leakage during severe dengue disease.

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“…High mobility group protein b1 (HMGB1) is a ubiquitous nuclear protein that is released extracellularly after cell activation, stress, injury, or apoptosis and plays an important role in neurodegenerative diseases by mediating neuroinflammation 103,104 ;…”

Section: Diabetes-related Cognitive Decline and Pyroptosismentioning

confidence: 99%

Trend Research of Diabetes and Pyroptosis: Bibliometric Analysis and Literature Review

Li¹,

Xiaojuan²,

Mingchao³

2023

Preprint

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Objective: To visualize and analyze the published literature on diabetes and pyroptosis based on a bibliometric approach, so as to provide a comprehensive picture of the hot research directions and dynamic progress in this field. Methods: This study was based on the web of science core collection database to conduct a comprehensive search of the published literature in the field of diabetes and Pyroptosis from January 1985 to August 2022, including the published research literature in this field, as well as a visual analysis of the number of citations, year of publication, journal, author, research institution, country, and research topic. Results: A total of 139 literature on research related to diabetes and cellular scorch from 2011 to 2022 were retrieved, with a total of 3009 citations and a maximum of 255 citations for a single article, which had a first authorSchmid-Burgk, JL The first author of this article is from Germany; among 20 publishing countries, China leads with 100 articles; among 222 publishing institutions, Harbin Medical University leads with 18 articles and 184 citations; among 980 authors, Chen, X from China tops the list of high-impact authors with 5 articles and 29 citations; Among the 98 journals, "CELL DEATH DISEASE" ranked first in both volume and high impact journals with 4 articles and 29 citations; among 349 keywords, "pyroptosis" ranked first with a cumulative frequency of 65 times. The cluster analysis was divided into three categories, chronic complications of diabetes and pyroptosis (67 articles), diabetes and pyroptosis (60 articles), and diabetes combined with other diseases and pyroptosis (12 articles), and the number of articles related to diabetes and its chronic complications increased rapidly from 2019, among which, diabetic cardiomyopathy (27 articles) had the highest number of articles. Conclusions: Based on a comprehensive analysis of published literature in the field of diabetes and pyroptosis from 2011-2022, this study achieved a visual analysis of studies with significant and outstanding contributions to the field, thus framing a picture showing the development and changes in the field. At the same time, this study provides research information and direction for clinicians and investigators to conduct diabetes and pyroptosis related research in the future.

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Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion

Cited by 12 publications

References 39 publications

Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity

Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity

Flavivirus NS1 Triggers Tissue-Specific Disassembly of Intercellular Junctions Leading to Barrier Dysfunction and Vascular Leak in a GSK-3β-Dependent Manner

Trend Research of Diabetes and Pyroptosis: Bibliometric Analysis and Literature Review

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