Bowman-Birk inhibitor concentrate prevents atrophy, weakness, and oxidative stress in soleus muscle of hindlimb-unloaded mice

Arbogast, Sandrine; Smith, Jacqueline; Matuszczak, Yves; Hardin, Brian J.; Moylan, Jennifer S.; Smith, Jeffrey D.; Ware, Jeffrey H.; Kennedy, Ann R.; Reid, Michael B.

doi:10.1152/japplphysiol.00538.2006

Cited by 67 publications

(67 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6) suggests a source nearby (e.g., Nox2, subsarcolemmal mitochondria). Previously, Abrogast et al (4) reported that oxidative stress was elevated in unloaded skeletal muscle after 1-3 days. Bowman-Birk inhibitor complex (BBIC), which exhibits antiproteasome and antioxidant properties, significantly reduced unloading-induced fiber atrophy and reduction of force-generating capacity (4).…”

Section: Discussionmentioning

confidence: 98%

“…Indeed, while some antioxidant strategies have been successful in reducing skeletal muscle atrophy with mechanical unloading (4,25,30), other interventions have not (6,20). For example, administration of BBIC significantly attenuated muscle atrophy in limb muscles (4), while Trolox was less successful (6).…”

Section: Discussionmentioning

confidence: 99%

“…Muscle atrophy that occurs with unloading is coupled to a net loss of contractile proteins, a function of increased protein degradation combined with a decrease in protein synthesis (12,23). Recent studies emphasize the importance of proteolytic pathways, including ubiquitin proteasome, calpains, autophagy, and caspase-3 in triggering the early stages of atrophy (4,10,11,26,34,35,39). For example, elevation of ubiquitin ligases with unloading is initiated via dephosphorylation of FoxO3a and activation of nuclear factor-B (NF-B) (26,34,42).…”

mentioning

confidence: 99%

See 2 more Smart Citations

EUK-134 ameliorates nNOSμ translocation and skeletal muscle fiber atrophy during short-term mechanical unloading

Lawler

Kunst

Hord

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

. EUK-134 ameliorates nNOS translocation and skeletal muscle fiber atrophy during short-term mechanical unloading. Am J Physiol Regul Integr Comp Physiol 306: R470-R482, 2014. First published January 29, 2014 doi:10.1152/ajpregu.00371.2013.-Reduced mechanical loading during bedrest, spaceflight, and casting, causes rapid morphological changes in skeletal muscle: fiber atrophy and reduction of slow-twitch fibers. An emerging signaling event in response to unloading is the translocation of neuronal nitric oxide synthase (nNOS) from the sarcolemma to the cytosol. We used EUK-134, a cellpermeable mimetic of superoxide dismutase and catalase, to test the role of redox signaling in nNOS translocation and muscle fiber atrophy as a result of short-term (54 h) hindlimb unloading. Fischer-344 rats were divided into ambulatory control, hindlimb-unloaded (HU), and hindlimb-unloaded ϩ EUK-134 (HU-EUK) groups. EUK-134 mitigated the unloading-induced phenotype, including muscle fiber atrophy and muscle fiber-type shift from slow to fast. nNOS immunolocalization at the sarcolemma of the soleus was reduced with HU, while nNOS protein content in the cytosol increased with unloading. Translocation of nNOS from the sarcolemma to cytosol was virtually abolished by EUK-134. EUK-134 also mitigated dephosphorylation at Thr-32 of FoxO3a during HU. Hindlimb unloading elevated oxidative stress (4-hydroxynonenal) and increased sarcolemmal localization of Nox2 subunits gp91phox (Nox2) and p47phox, effects normalized by EUK-134. Thus, our findings are consistent with the hypothesis that oxidative stress triggers nNOS translocation from the sarcolemma and FoxO3a dephosphorylation as an early event during mechanical unloading. Thus, redox signaling may serve as a biological switch for nNOS to initiate morphological changes in skeletal muscle fibers. atrophy; disuse; skeletal muscle; nNOS; oxidative stress; FoxO3a SKELETAL MUSCLE IS A HIGHLY specialized and adaptable mesodermic tissue capable of rapid remodeling in response to changes in mechanical loading and stretch (28, 38). Transmittance and detection of loading in skeletal muscle are paramount in regulating differentiation, cell growth, and protein turnover. The ability to sense and relay loading (i.e., mechanotransduction) is, in part, performed by proteins adjacent to and spanning cell membranes (i.e., sarcolemma) in skeletal muscle (47). Mechanical unloading that occurs with limb casting, splinting, bed rest, and spaceflight, elicits a substantial loss of forcegenerating capacity, linked to a diminishment in muscle fiber cross-sectional area or atrophy (12, 23). The unloading phenotype also includes a shift of a portion of skeletal muscle fibers from slow-twitch to fast-twitch (12). Muscle atrophy that occurs with unloading is coupled to a net loss of contractile proteins, a function of increased protein degradation combined with a decrease in protein synthesis (12,23). Recent studies emphasize the importance of proteolytic pathways, including ubiquitin proteasome, calpains, autophagy...

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

EUK-134 ameliorates nNOSμ translocation and skeletal muscle fiber atrophy during short-term mechanical unloading

Lawler

Kunst

Hord

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…vitamin E or soya protein extract) has been published (14,101,187,188) . However, contradictory results have been obtained regarding the impact of vitamin E on muscle atrophy.…”

Section: Nutritional Interventions To Reduce the Anabolism Threshold mentioning

confidence: 99%

Nutritional strategies to counteract muscle atrophy caused by disuse and to improve recovery

Magne¹,

Savary-Auzeloux²,

Rémond³

et al. 2013

Nutr. Res. Rev.

View full text Add to dashboard Cite

Periods of immobilisation are often associated with pathologies and/or ageing. These periods of muscle disuse induce muscle atrophy which could worsen the pathology or elderly frailty. If muscle mass loss has positive effects in the short term, a sustained/uncontrolled muscle mass loss is deleterious for health. Muscle mass recovery following immobilisation-induced atrophy could be critical, particularly when it is uncompleted as observed during ageing. Exercise, the best way to recover muscle mass, is not always applicable. So, other approaches such as nutritional strategies are needed to limit muscle wasting and to improve muscle mass recovery in such situations. The present review discusses mechanisms involved in muscle atrophy following disuse and during recovery and emphasises the effect of age in these mechanisms. In addition, the efficiency of nutritional strategies proposed to limit muscle mass loss during disuse and to improve protein gain during recovery (leucine supplementation, whey proteins, antioxidants and anti-inflammatory compounds, energy intake) is also discussed.

show abstract

“…Skeletal muscular atrophy can be reproduced experimentally in rodents by hind-limb unloading. Dietary supplementation containing 1% BBIC has been reported to inhibit unloading-induced weakness in mice [89], promoting redox homeostasis in muscle fibers and blunting atrophy-induced weakness [90]. Morris et al [89] suggested that inhibiting muscle degrading proteases may provide a new pharmacological strategy in treating skeletal m u s c u l a r a t r o p h y ; h o wever, such proteases remain uncharacterized.…”

Section: Beneficial Properties Of Bowman-birk Inhibitors In Non-relatmentioning

confidence: 99%

Bowman-Birk Inhibitors from Legumes: Utilisation in Disease Prevention and Therapy

Clemente¹,

Marín-Manzano²,

Arques³

et al. 2013

Bioactive Food Peptides in Health and Disease

View full text Add to dashboard Cite

Bowman-Birk inhibitor concentrate prevents atrophy, weakness, and oxidative stress in soleus muscle of hindlimb-unloaded mice

Abstract: inhibitor concentrate prevents atrophy, weakness, and oxidative stress in soleus muscle of hindlimb-unloaded mice.

Cited by 67 publications

References 36 publications

EUK-134 ameliorates nNOSμ translocation and skeletal muscle fiber atrophy during short-term mechanical unloading

EUK-134 ameliorates nNOSμ translocation and skeletal muscle fiber atrophy during short-term mechanical unloading

Nutritional strategies to counteract muscle atrophy caused by disuse and to improve recovery

Bowman-Birk Inhibitors from Legumes: Utilisation in Disease Prevention and Therapy

Contact Info

Product

Resources

About