Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds

Chen, Xing; Cheng, Zhangrui; Werling, Dirk; Pollott, G.E.; Salavati, Mazdak; Johnson, Kate F.; Khan, Faheem Ahmed; Wathes, D C; Zhang, Shujun

doi:10.1016/j.rvsc.2016.08.004

Cited by 5 publications

(10 citation statements)

References 62 publications

(57 reference statements)

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“…As described previously [18], we initially identified 13 SNP in the exons of three selectin genes of a defined group of British Holstein Friesian cows. SELP was found to have the highest level of polymorphism as nine of the 13 SNPs were located in SELP exons, including three missense mutations.…”

Section: Resultsmentioning

confidence: 99%

“…This is highly likely to be driven by host-pathogen co-evolution, such as to malaria parasites, as SELP is involved in the adherence of Plasmodium falciparum -infected erythrocytes to vascular-endothelial cells [57]. Similar to the human system, we have shown previously that SELP is also highly polymorphic in the dairy cow population with a significant reversal in the breed distribution of SELP Ex13 between Holstein and Friesian or Jersey cows [18]. …”

Section: Discussionmentioning

confidence: 99%

“…The method used for SNP identification has been described previously [18]. DNA from blood samples was extracted using a FlexiGene DNA Kit (Qiagen, North Manchester, UK) following the protocol supplied and quantified with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc. Wilmington, USA).…”

Section: Methodsmentioning

confidence: 99%

“…We showed previously using an in vitro system that pre-activation of cultured bovine aortic endothelial cells (BoAEC) with lipopolysaccharide (LPS) caused a rapid (10 min) and slower (4 h) enhancement of adhesion by bovine peripheral blood leukocytes. This adhesion was accompanied by an up-regulation of both SELP and SELE mRNA expression in BoAEC and could be blocked using antibody against SELP [18]. …”

Section: Introductionmentioning

confidence: 99%

“…Both this analysis and some of the disease association studies have provided evidence for a diversity in genotype frequencies for SNP in SELL and SELP according to European, Asian or African ethnicity [29, 32, 33]. In the cow, we previously investigated the presence of gene polymorphism for bovine selectins in a population of mainly British Holstein Friesian cows, together with several other dairy breeds (Friesian, Jersey, Ayrshire and Brown Swiss) [18]. SELP was highly polymorphic, with nine of the 13 SNPs identified located in its exons, whereas only three synonymous SNPs were found in SELL and one in SELE .…”

Section: Introductionmentioning

confidence: 99%

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Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows

et al. 2017

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Selectins are adhesion molecules, which mediate attachment between leucocytes and endothelium. They aid extravasation of leucocytes from blood into inflamed tissue during the mammary gland’s response to infection. Selectins are also involved in attachment of the conceptus to the endometrium and subsequent placental development. Poor fertility and udder health are major causes for culling dairy cows. The three identified bovine selectin genes SELP, SELL and SELE are located in a gene cluster. SELP is the most polymorphic of these genes. Several SNP in SELP and SELE are associated with human vascular disease, while SELP SNP rs6127 has been associated with recurrent pregnancy loss in women. This study describes the results of a gene association study for SNP in SELP (n = 5), SELL (n = 2) and SELE (n = 1) with fertility, milk production and longevity traits in a population of 337 Holstein Friesian dairy cows. Blood samples for PCR-RFLP were collected at 6 months of age and animals were monitored until either culling or 2,340 days from birth. Three SNP in SELPEx4-6 formed a haplotype block containing a Glu/Ala substitution at rs42312260. This region was associated with poor fertility and reduced survival times. SELPEx8 (rs378218397) coded for a Val475Met variant locus in the linking region between consensus repeats 4 and 5, which may influence glycosylation. The synonymous SNP rs110045112 in SELEEx14 deviated from Hardy Weinberg equilibrium. For both this SNP and rs378218397 there were too few AA homozygotes present in the population and AG heterozygotes had significantly worse fertility than GG homozygotes. Small changes in milk production associated with some SNP could not account for the reduced fertility and only SELPEx6 showed any association with somatic cell count. These results suggest that polymorphisms in SELP and SELE are associated with the likelihood of successful pregnancy, potentially through compromised implantation and placental development.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows

et al. 2017

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Endothelial Cells Exhibit Two Waves of P-selectin Surface Aggregation Under Endotoxic and Oxidative Conditions

et al. 2019

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Metabolomic Profiling of Bos taurus Beef, Dairy, and Crossbred Cattle: A Between-Breeds Meta-Analysis

Gómez

Salvetti²,

Gatien³

et al. 2020

J. Agric. Food Chem.

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Cattle breeds may differ substantially in their metabolism. However, the metabolomes of dairy and beef cattle are not well-known. Knowledge of breed-specific metabolic features is essential for biomarker identification and to adopt specific nutritional strategies. The muscle hypertrophy (mh), a beef cattle phenotype present in Asturiana de los Valles (AV) but absent in Asturiana de la Montaña (AM) and Holsteins, may underlie such differences. We compared the plasma metabolomes of Holstein, AV, AM, and crossbred cattle recipients selected for meta-analysis within an embryo transfer (ET) program. Blood samples were collected on day 0 (oestrus) and day 7 (prior to ET) (N = 234 samples × 2 days). Nuclear magnetic resonance quantified N = 36 metabolites in plasma, and more metabolic differences between breeds were found on day 0 (N = 19 regulated metabolites) than on day 7 (N = 5). AV and AM largely differed from Holstein cattle (N = 55 and 35 enriched metabolic pathways, respectively); however, AV and AM differed in N = 6 enriched pathways. Metabolic activity was higher in AV than in Holstein cattle, as explained in part by the mh phenotype. The metabolomic characterization of breeds facilitates biomarker research and helps to define the healthy ranges of metabolite concentrations.

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Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds

Cited by 5 publications

References 62 publications

Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows

Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows

Endothelial Cells Exhibit Two Waves of P-selectin Surface Aggregation Under Endotoxic and Oxidative Conditions

Metabolomic Profiling of Bos taurus Beef, Dairy, and Crossbred Cattle: A Between-Breeds Meta-Analysis

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