Bovine milk extracellular vesicles induce the proliferation and differentiation of osteoblasts and promote osteogenesis in rats

Go, Gyeongyun; Jeon, Juhee; Lee, Ga Eun; Lee, Jun Hee

doi:10.1111/jfbc.13705

Cited by 19 publications

(26 citation statements)

References 42 publications

(45 reference statements)

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“…In an ensuing study, Oliveira et al showed that milk exosomes treatment on murine bone marrow cells induced an increase in osteoclast differentiation along with an inhibition of the osteoclast activity [ 149 ]. Recent research conducted by Go et al studied the addition of bovine milk exosomes to human osteoblastic Saos-2 cells in vitro, observing an increase in Saos-2 cells proliferation in a time-dependent and dose-dependent manner while promoting the expression of the osteoblast transcription factors RUNX2 and Osterix [ 150 ]. Moreover, treatment of Saos-2 and MC3T3-E1 pre-osteoblastic cells with milk exosomes increased alkaline phosphatase (ALP) and osteocalcin (OCN) levels, which are involved in extracellular matrix synthesis processes [ 150 ].…”

Section: Beneficial Effects Of Bovine Milk Exosomes On Overall Healthmentioning

confidence: 99%

“…Recent research conducted by Go et al studied the addition of bovine milk exosomes to human osteoblastic Saos-2 cells in vitro, observing an increase in Saos-2 cells proliferation in a time-dependent and dose-dependent manner while promoting the expression of the osteoblast transcription factors RUNX2 and Osterix [ 150 ]. Moreover, treatment of Saos-2 and MC3T3-E1 pre-osteoblastic cells with milk exosomes increased alkaline phosphatase (ALP) and osteocalcin (OCN) levels, which are involved in extracellular matrix synthesis processes [ 150 ]. Finally, the authors administered 50 mg/kg/day of exosomes to rats for 14 days and reported that the treatment was associated with significantly increased bone mineral density in trabecular and cortical tibiae [ 150 ].…”

Section: Beneficial Effects Of Bovine Milk Exosomes On Overall Healthmentioning

confidence: 99%

“…Moreover, treatment of Saos-2 and MC3T3-E1 pre-osteoblastic cells with milk exosomes increased alkaline phosphatase (ALP) and osteocalcin (OCN) levels, which are involved in extracellular matrix synthesis processes [ 150 ]. Finally, the authors administered 50 mg/kg/day of exosomes to rats for 14 days and reported that the treatment was associated with significantly increased bone mineral density in trabecular and cortical tibiae [ 150 ]. In the light of these studies, bovine milk exosomes could constitute food components with the potential to influence different aspects of bone remodeling.…”

Section: Beneficial Effects Of Bovine Milk Exosomes On Overall Healthmentioning

confidence: 99%

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Beneficial Effects of Bovine Milk Exosomes in Metabolic Interorgan Cross-Talk

et al. 2022

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Extracellular vesicles are membrane-enclosed secreted vesicles involved in cell-to-cell communication processes, identified in virtually all body fluids. Among extracellular vesicles, exosomes have gained increasing attention in recent years as they have unique biological origins and deliver different cargos, such as nucleic acids, proteins, and lipids, which might mediate various health processes. In particular, milk-derived exosomes are proposed as bioactive compounds of breast milk, which have been reported to resist gastric digestion and reach systemic circulation, thus being bioavailable after oral intake. In the present manuscript, we critically discuss the available evidence on the health benefits attributed to milk exosomes, and we provide an outlook for the potential future uses of these compounds. The use of milk exosomes as bioactive ingredients represents a novel avenue to explore in the context of human nutrition, and they might exert important beneficial effects at multiple levels, including but not limited to intestinal health, bone and muscle metabolism, immunity, modulation of the microbiota, growth, and development.

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Section: Beneficial Effects Of Bovine Milk Exosomes On Overall Healthmentioning

confidence: 99%

Section: Beneficial Effects Of Bovine Milk Exosomes On Overall Healthmentioning

confidence: 99%

Section: Beneficial Effects Of Bovine Milk Exosomes On Overall Healthmentioning

confidence: 99%

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Beneficial Effects of Bovine Milk Exosomes in Metabolic Interorgan Cross-Talk

et al. 2022

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“… [ 258 ] Cow Promotion of osteogenesis via proliferation and differentiation of osteoblasts in vitro (Saos-2 cell line) and in vivo (Sprague Dawley rats). [ 259 ] Cow Improved small intestinal dysfunction in malnutrition C57BL/6J mouse model. [ 260 ] Cow Enhancement of curcumin cell uptake and permeability in an intestinal model in vitro (Caco-2 cell line).…”

Section: Biological Effects Of Milk-derived Evsmentioning

confidence: 99%

Ruminant Milk-Derived Extracellular Vesicles: A Nutritional and Therapeutic Opportunity?

et al. 2021

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Milk has been shown to contain a specific fraction of extracellular particles that are reported to resist digestion and are purposefully packaged with lipids, proteins, and nucleic acids to exert specific biological effects. These findings suggest that these particles may have a role in the quality of infant nutrition, particularly in the early phase of life when many of the foundations of an infant’s potential for health and overall wellness are established. However, much of the current research focuses on human or cow milk only, and there is a knowledge gap in how milk from other species, which may be more commonly consumed in different regions, could also have these reported biological effects. Our review provides a summary of the studies into the extracellular particle fraction of milk from a wider range of ruminants and pseudo-ruminants, focusing on how this fraction is isolated and characterised, the stability and uptake of the fraction, and the reported biological effects of these fractions in a range of model systems. As the individual composition of milk from different species is known to differ, we propose that the extracellular particle fraction of milk from non-traditional and minority species may also have important and distinct biological properties that warrant further study.

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“…Regulation of BMP2/CBFA1 signaling molecules by SOS induced the construction of bone mineralized matrix. In postmenopausal osteoporosis, the homeostasis between osteoblast and osteoclast is destructed due to estrogen deficiency, leading to decline of osteogenesis (Eastell et al, 2016; Go, Jeon, Lee, Lee, & Lee, 2021). We found that SOS promoted osteogenesis BMP2 involved signaling in postmenopausal osteoporotic mice.…”

Section: Resultsmentioning

confidence: 99%

Promotion of osteogenesis by Sweroside via BMP2‐involved signaling in postmenopausal osteoporosis

Choi

Kim

2021

Phytotherapy Research

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Phlomis umbrosa has been traditionally used for bone diseases in traditional Korean Medicine. Sweroside (SOS), marker compounds of P. umbrosa, has been known to promote osteoblast differentiation. In this study, ameliorative effects of SOS on osteoporosis and potential target pathway were investigated. Ovariectomized mice were administered three doses of SOS three times a week for 4 weeks after inducing osteoporosis. Bone mineral content (BMC) and bone mineral density (BMD) were analyzed by dual energy X‐ray absorptiometry. A human osteosarcoma cell line (SaOS‐2) was differentiated to clarify the promoting effects of SOS on osteoblast differentiation and bone formation. Osteoblastic bone‐forming markers were evaluated in lumbar vertebrae (LV) and mineralized SaOS‐2 cells. SOS markedly elevated BMC and BMD levels and attenuated the bone marrow adipocytes in the femoral shaft. SOS increased the formation of bone matrix in SaOS‐2 cells. Bone morphogenetic protein‐2 (BMP2) and runt‐related transcription factor 2 (CBFA1) in LV and SaOS‐2 cells were up‐regulated by SOS. SOS increased alkaline phosphatase (ALPL), osteopontin (SPP1), and bone sialoprotein‐1 (BSPH1). In conclusion, SOS induced the formation of mineralized bone matrix by regulating BMP2/CBFA1‐mediated molecules. Therefore, SOS could be a therapeutic compound of treatment for osteoporosis by producing the new bone matrix.

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Bovine milk extracellular vesicles induce the proliferation and differentiation of osteoblasts and promote osteogenesis in rats

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 19 publications

References 42 publications

Beneficial Effects of Bovine Milk Exosomes in Metabolic Interorgan Cross-Talk

Beneficial Effects of Bovine Milk Exosomes in Metabolic Interorgan Cross-Talk

Ruminant Milk-Derived Extracellular Vesicles: A Nutritional and Therapeutic Opportunity?

Promotion of osteogenesis by Sweroside via BMP2‐involved signaling in postmenopausal osteoporosis

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