Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption

Olaya-Galán, Nury Nathalia; Corredor-Figueroa, Adriana P.; Guzmán-Garzón, T C; Ríos-Hernandez, K S; Salas-Cárdenas, Sandra P.; Patarroyo, Manuel A.; Gutiérrez, María Lucía Lahoz

doi:10.1017/s0950268817002229

Cited by 43 publications

(42 citation statements)

References 39 publications

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“…BLV is endemic in cattle in many countries where it causes leukosis in 2-5% of infected animals [109]. Its presence in meat and dairy products has been shown [110] and Buehring et al (2003) [111] found antibodies against BLV capsid antigen in 74% of human serum samples using immunoblotting. There are also several reports claiming an association of breast or colon cancer with BLV [73,112].…”

Section: Foodborne Animal Viruses With Unclear Zoonotic Potentialmentioning

confidence: 99%

Selected Viruses Detected on and in our Food

Bachofen

2018

Curr Clin Micro Rpt

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Purpose of Review The purpose of this review is to provide an update on recent literature and findings concerning selected foodborne viruses. Two groups of viruses were selected: (a) the most important viruses contaminating food, based on numbers of publications in the last 5 years and (b) viruses infecting sources of food that might have an impact on human health. Recent Findings Important foodborne viruses such as norovirus, hepatitis A and rotavirus are usually "only" contaminating food and are detected on the surface of foodstuffs. However, they are threats to human public health and make up for the majority of cases. In contrast, the meaning of viruses born from within the food such as natural animal and plant viruses is still in many cases unknown. An exception is Hepatitis E virus that is endemic in pigs, transmitted via pork meat and is recognised as an emerging zoonosis in industrialised countries. Summary Even though the clinical meaning of "new" foodborne viruses, often detected by next generation sequencing, still needs clarification, the method has great potential to enhance surveillance and detection particularly in view of an increasingly globalised food trade.

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Section: Foodborne Animal Viruses With Unclear Zoonotic Potentialmentioning

confidence: 99%

Selected Viruses Detected on and in our Food

Bachofen

2018

Curr Clin Micro Rpt

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“…Previous studies report the presence of BLV in the bovine mammary epihtelium (Buehring et al, 1994;Yoshikawa et al, 1997). However, most recent works are focused in the detection of BLV infected mononuclear cells in milk (Jaworski et al, 2016;Kuckleburg et al, 2003), and its potential risk to human (Olaya-Galán et al, 2017;Sellers et al, 2008).…”

mentioning

confidence: 99%

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

et al. 2018

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Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production in cattle. The aim of this study was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfully infected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blot and transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stably infected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immune response in the mammary gland and the relationship with other agents causing mastitis.

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“…In the other hand, variability of genotypes as well as results of structural and functional properties of its envelope proteins [36] provide useful information for research focused on vaccines production with specific strains, that in the case of Colombia, will be appropriate to include conserved epitopes shared between genotypes 1, 6 and 3 according to the results obtained in this study and previously reported [31]. In addition, phylogenetic studies are important to understand the geographical distribution of the virus and thus, identify conserved and hypervariable regions including specific mutations that could be related with different levels of virulence or pathogenicity [6,[37][38][39][40][41].…”

Section: Discussionmentioning

confidence: 82%

“…Given the importance of cattle industry in the country and in Latin America, it would be relevant if governmental entities and policy-maker agencies consider including the enzootic bovine leukosis as an official control disease, facilitating the diagnosis to the cattle industry members to identify infected animals, and furthermore, to control the dissemination of the virus focused in the future on the promotion of eradication programs, and likewise, minimizing the impact that BLV has on cattle and eventually on humans who consume bovine-derived food products [37].…”

Section: Discussionmentioning

confidence: 99%

Masked Genotype 6 of Bovine Leukemia Virus Is Found in Colombian Cattle

Corredor-Figueroa

Olaya-Galán

Salas

et al. 2019

Preprint

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Background Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. Results A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. Conclusions This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities.

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Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption

Cited by 43 publications

References 39 publications

Selected Viruses Detected on and in our Food

Selected Viruses Detected on and in our Food

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

Masked Genotype 6 of Bovine Leukemia Virus Is Found in Colombian Cattle

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