Bovine Fetal Hemoglobin. I. Postnatal Persistence and Relation to Adult Hemoglobins

Grimes, R.M.; Duncan, C.W.; Lassiter, C. A.

doi:10.3168/jds.s0022-0302(58)91130-5

Cited by 37 publications

(15 citation statements)

References 18 publications

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“…For example, the detection of foetal haemoglobin, which is only expressed in animals younger than a few months [64], suggests that the cape from Møgelmose, was produced from a calf slaughtered within a few months of birth. A skin element from this cape was previously identified as the genus Martes [11], thus the present analysis represents crucial new information, also on preferences for specific qualities for production, as calfskin is much softer than skins from older animals.…”

Section: Discussionmentioning

confidence: 99%

Species Identification of Archaeological Skin Objects from Danish Bogs: Comparison between Mass Spectrometry-Based Peptide Sequencing and Microscopy-Based Methods

et al. 2014

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Denmark has an extraordinarily large and well-preserved collection of archaeological skin garments found in peat bogs, dated to approximately 920 BC – AD 775. These objects provide not only the possibility to study prehistoric skin costume and technologies, but also to investigate the animal species used for the production of skin garments. Until recently, species identification of archaeological skin was primarily performed by light and scanning electron microscopy or the analysis of ancient DNA. However, the efficacy of these methods can be limited due to the harsh, mostly acidic environment of peat bogs leading to morphological and molecular degradation within the samples. We compared species assignment results of twelve archaeological skin samples from Danish bogs using Mass Spectrometry (MS)-based peptide sequencing, against results obtained using light and scanning electron microscopy. While it was difficult to obtain reliable results using microscopy, MS enabled the identification of several species-diagnostic peptides, mostly from collagen and keratins, allowing confident species discrimination even among taxonomically close organisms, such as sheep and goat. Unlike previous MS-based methods, mostly relying on peptide fingerprinting, the shotgun sequencing approach we describe aims to identify the complete extracted ancient proteome, without preselected specific targets. As an example, we report the identification, in one of the samples, of two peptides uniquely assigned to bovine foetal haemoglobin, indicating the production of skin from a calf slaughtered within the first months of its life. We conclude that MS-based peptide sequencing is a reliable method for species identification of samples from bogs. The mass spectrometry proteomics data were deposited in the ProteomeXchange Consortium with the dataset identifier PXD001029.

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Section: Discussionmentioning

confidence: 99%

Species Identification of Archaeological Skin Objects from Danish Bogs: Comparison between Mass Spectrometry-Based Peptide Sequencing and Microscopy-Based Methods

et al. 2014

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“…It is likely that young calves have a higher number of young erythrocytes which contain more magnesium than old erythrocytes (BERNSTEIN, 1959;GINSBURG et al, 1962) in early life. In could also be that foetal haemoglobin (HbF) (GRIMES et al, 1958) has a higher affinity for magnesium. This would lead to high levels of bound magnesium, i. e. unexchangeable magnesium (CARE, 1960).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore any type of haemoglobin with high affinity for magnesium would lead to high levels of bound (unexchangeable) EMg concentrations (CARE, 1960). It is also possible that in a newborn calf, the foetal erythrocytes which are replaced within 2-3 months (GRIMES et al, 1958) have a higher metabolic rate and hence are able to maintain higher EMg concentrations than adult erythrocytes. Erythrocytes with high metabolic rate have a higher EMg concentration than those with low metabolic rate (BERNSTEIN, 1959;GINS-BURG et al, 1962).…”

Section: Discussionmentioning

confidence: 99%

Changes in Concentrations of Certain Plasma and Erythrocyte Minerals Associated with Hypomagnesaemia in Young Calves

Mulei

Daniel

1989

Journal of Veterinary Medicine Series A

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Summary Hypomagnesaemia was induced in young calves 1–2 weeks old by feeding them with a diet composed of skimmed milk and barley straw to which urea, potassium chloride and soya‐bean oil were added. Blood samples were collected from each calf once a week from the jugular vein until there was a significantly lower (P < 0.01) plasma magnesium (PMg) concentration in the experimental compared to the control calves receiving MgO instead of KCl. In addition to PMg, the blood samples were analysed for plasma sodium, potassium, calcium, inorganic phosphate and erythrocyte concentrations of magnesium, sodium and potassium and red blood cell counts. The erythrocyte magnesium (EMg) concentration decreased significantly (P < 0.01) in both groups. The decrease was, however, greater (P < 0.05) in the magnesium deficient group than in the control group. Erythrocyte sodium (ENa) concentration increased and erythrocyte potassium (EK) concentration decreased in both groups, and the changes did not differ significantly between the two groups. Plasma concentrations of sodium (PNa) and potassium (PK) and red blood cell counts did not change significantly in either group. Significant (P < 0.05) decreases in plasma calcium and inorganic phosphate concentrations occurred in the magnesium deficient group only. Zusammenfassung Veränderungen von Mineralstoff‐Konzentrationen im Plasma und in den Erythrozyten bei der Hypomagnesämie junger Kälber Bei 1–2 Wochen alten Kälbern wurde durch Verfütterung von Magermilch und Gerstenstroh mit Harnstoff‐Kaliumchlorid‐ und Sojaöl‐Zusatz eine Hypomagnesämie induziert. Dabei wurde einmal wöchentlich bei jedem Kalb eine Blutprobe aus der Vena jugularis entnommen, bis sich ein im Vergleich zu Kontrollkälbern, die anstatt KCl MgO erhielten, ein signifikant niedrigerer (p < 0,01) Plasma‐Magnesium‐(PMg)‐Spiegel ergab. Neben dem PMg wurden der Plasma‐Natrium‐, ‐Kalium‐, ‐Calcium‐ und ‐Phosphat‐Spiegel sowie die Konzentrationen von Magnesium, Natrium und Kalium in den Erythrozyten bestimmt. Die Zahl der Erythrozyten wurde ebenfalls ermittelt. Die Magnesium‐Konzentration in den Erythrozyten (EMg) nahm bei beiden Gruppen signifikant (p < 0,01) ab. Diese Abnahme war jedoch bei der Magnesium‐Mangelgruppe größer. Die Natrium‐Konzentration in den Erythrozyten (ENa) erhöhte sich, und die Kalium‐Konzentration in den Erythrozyten (EK) erniedrigte sich in beiden Gruppen. Dabei ergaben sich keine signifikanten Gruppenunterschiede. Die Plasma‐Natrium‐(PNa)‐ und Plasma‐Kalium‐(PK)‐Konzentrationen sowie die Zahl der Erythrozyten veränderten sich in keiner der Gruppen signifikant. Eine signifikante (p < 0,05) Abnahme der Plasma‐Calcium‐ und ‐Phosphat‐Konzentration ergab sich bei der Magnesium‐Mangelgruppe.

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“…In most animals it is completely replaced within 65 to 124 days, but in two Guernsey bull calves it persists beyond 125 days, indicating that breed, age, sex and individual differences exist in cattle. 4 Foetal haemoglobin was also found to the extent of 7.45% in adult hill cattle reared at an altitude of 1500 to 2000 m. 5 The natural habitat of yaks is between 3000 and 6000 m above sea level. The oxygen pressure (PO 2 ) at 3000 m is 68% of the value at sea level and it further decreases with increase in altitude.…”

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confidence: 99%

Postnatal persistence of foetal haemoglobin in yaks

Sarkar

et al. 1999

Aust Veterinary J

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Bovine Fetal Hemoglobin. I. Postnatal Persistence and Relation to Adult Hemoglobins

Cited by 37 publications

References 18 publications

Species Identification of Archaeological Skin Objects from Danish Bogs: Comparison between Mass Spectrometry-Based Peptide Sequencing and Microscopy-Based Methods

Species Identification of Archaeological Skin Objects from Danish Bogs: Comparison between Mass Spectrometry-Based Peptide Sequencing and Microscopy-Based Methods

Changes in Concentrations of Certain Plasma and Erythrocyte Minerals Associated with Hypomagnesaemia in Young Calves

Postnatal persistence of foetal haemoglobin in yaks

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