volume 66, issue 6, P3373-3384 1992
DOI: 10.1128/jvi.66.6.3373-3384.1992
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Abstract: We have localized a cis-acting sequence that promotes initiation of lytic-phase DNA replication (oriLyt) within the Hindlll D fragment of the human cytomegalovirus (HCMV) AD169 genome and investigated its sequence requirements by testing the ability of plasmid constructs to mediate DNA replication in a transient transfection-plus-infection assay. Replication of plasmids containing HCMV oriLyt required at least the virus-specified DNA polymerase activity supplied by HCMV infection of transfected cells and was a…

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