Both silver ions and silver nanoparticles facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes

Lu, Ji; Wang, Yue; Jin, Min; Yuan, Zhiguo; Bond, Philip L.; Guo, Jianhua

doi:10.1016/j.watres.2019.115229

Cited by 239 publications

(116 citation statements)

References 70 publications

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“…The heavy metal NPs/ions-promoted natural transformation was found to be associated with ROS overproduction, as validated by the reversal of the transformation frequency to baseline levels with the addition of a ROS scavenger-thiourea ( Figure 2G), with ZnO NPs as an exception. The observed transformation promoted by heavy metal-based NPs/ions was in agreement with our previous findings that CuO and Ag NPs/ions could enhance conjugative ARGs transfer from Escherichia coli to Pseudomonas putida via over-generation of ROS (18,19) Likewise, recent studies have also indicated that solar disinfection and water disinfection by-products could increase the natural transformation rates of extracellular DNA in A. baylyi via inducing ROS (24,25).…”

Section: Heavy Metal Nanoparticles and Ions Promoted Args Transformatsupporting

confidence: 91%

“…For instance, heavy metal ions (e.g., Cu 2+ , Cd 2+ , Hg 2+ and Zn 2+ ) and nanoparticles (e.g., ZnO NPs, Al 2 O 3 NPs and Ag NPs) have been reported to induce antibiotic resistance via co-selection or direct mutation [12,13,15,16]. In addition, heavy metal ions (including Cu 2+ , Ag + , Cr 6+ , Ti 4+ and Zn 2+ ) [17][18][19] and nanoparticles (e.g., Al 2 O 3 NPs, CuO NPs, Ag NPs and TiO 2 NPs) [18][19][20][21] could promote the transfer of ARGs via conjugation. However, it is unclear whether heavy metal-based NPs and ions could enhance the dissemination of ARGs via natural transformation.…”

Section: Introductionmentioning

confidence: 99%

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Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Guo

Zhang

et al. 2020

Preprint

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Background: Antibiotic resistance genes (ARGs), heavy metal ions and nanoparticles (NPs) are emerging and ubiquitous contaminants in the environment. However, little is known about whether heavy metal-based NPs or ions could facilitate the dissemination of ARGs through natural transformation. This study evaluated the contributions of heavy metal-based NPs (Ag NPs, CuO NPs and ZnO NPs) and their ion forms (Ag + , Cu 2+ and Zn 2+ ) to the transformation of extracellular ARGs in Acinetobacter baylyi ADP1. Results: We found that these commonly-used NPs and ions from environmentally relevant concentrations can significantly promote the natural transformation frequency of ARGs by a factor of 11.0-folds, which is comparable to the effects of antibiotics. The enhanced transformation by Ag NPs, CuO NPs, Ag + and Cu 2+ was primarily associated with reactive oxygen species (ROS) over-production and cell membrane damage, which was also evident from up-regulations of both transcription and translation of ROS and outer membrane-related genes. Additionally, transmission electron microscope imaging revealed the roughened cell membrane after Ag NPs, CuO NPs, Ag + and Cu 2+ exposure. ZnO NPs and Zn 2+ might increase the natural transformation rate by stimulating the stress response and ATP synthesis. All tested NPs and ions resulted in up-regulating the competence and SOS response-associated genes. Conclusions: Our results demonstrate that Ag, CuO and ZnO-based NPs/ions from environmental concentrations could promote the natural transformation of plasmid-encoded ARGs into naturally competent A. baylyi . Our findings provide insights into the contributions of heavy metals and NPs to the spread of antibiotic resistance.

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Section: Heavy Metal Nanoparticles and Ions Promoted Args Transformatsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Guo

Zhang

et al. 2020

Preprint

Self Cite

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“…The consequent effect is an accumulation of ions that leads to intracellular damage. However, it is known that both Gram +ve and Gram −ve bacteria have a negatively charged cell wall that allows for Few studies have reported the resistance of bacteria to MNPs and has been attributed to the development of extracellular substances that leads to agglomeration and precipitation of the MNPs [74][75][76][77]. Recently, the exposure of E. coli 013, E. coli CCM 3954 and P. aeruginosa to subinhibitory concentrations of AgNPs was studied.…”

Section: Cell Membrane: Lipid and Protein Interactionmentioning

confidence: 99%

Bactericidal Antibacterial Mechanism of Plant Synthesized Silver, Gold and Bimetallic Nanoparticles

Fanoro

Oluwafemi

2020

Pharmaceutics

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As the field of nanomedicine develops and tackles the recent surge in antibiotic resistance, there is a need to have an in-depth understanding and a synergistic view of research on the effectiveness of a metal nanoparticle (NP) as an antibacterial agent especially their mechanisms of action. The constant development of bacterial resistance has led scientists to develop novel antibiotic agents. Silver, gold and its bimetallic combination are one of the most promising metal NPs because they show strong antibacterial activity. In this review we discuss the mode of synthesis and the proposed mechanism of biocidal antibacterial activity of metal NPs. These mechanisms include DNA degradation, protein oxidation, generation of reactive oxygen species, lipid peroxidation, ATP depletion, damage of biomolecules and membrane interaction.

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“…In Escherichia coli and Pseudomonas putida, genes related to the general stress response were upregulated. Genes protecting against hydrogen peroxide oxidative damage, catalase/hydroperoxidase, superoxide radicals degradation genes, superoxide dismutase, and superoxide removal transcriptional activator, were upregulated in a range varying from 3.2-fold to 9.2-fold after a 2 h incubation period with Ag NPs [171].…”

Section: Changes In Expression Of Metabolic Genesmentioning

confidence: 99%

Antimicrobial metal-based nanoparticles: a review on their synthesis, types and antimicrobial action

Correa

Martínez

Vidal

et al. 2020

Beilstein J. Nanotechnol.

106

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The investigation of novel nanoparticles with antimicrobial activity has grown in recent years due to the increased incidence of nosocomial infections occurring during hospitalization and food poisoning derived from foodborne pathogens. Antimicrobial agents are necessary in various fields in which biological contamination occurs. For example, in food packaging they are used to control food contamination by microbes, in the medical field the microbial agents are important for reducing the risk of contamination in invasive and routine interventions, and in the textile industry, they can limit the growth of microorganisms due to sweat. The combination of nanotechnology with materials that have an intrinsic antimicrobial activity can result in the development of novel antimicrobial substances. Specifically, metal-based nanoparticles have attracted much interest due to their broad effectiveness against pathogenic microorganisms due to their high surface area and high reactivity. The aim of this review was to explore the state-of-the-art in metal-based nanoparticles, focusing on their synthesis methods, types, and their antimicrobial action. Different techniques used to synthesize metal-based nanoparticles were discussed, including chemical and physical methods and “green synthesis” methods that are free of chemical agents. Although the most studied nanoparticles with antimicrobial properties are metallic or metal-oxide nanoparticles, other types of nanoparticles, such as superparamagnetic iron-oxide nanoparticles and silica-releasing systems also exhibit antimicrobial properties. Finally, since the quantification and understanding of the antimicrobial action of metal-based nanoparticles are key topics, several methods for evaluating in vitro antimicrobial activity and the most common antimicrobial mechanisms (e.g., cell damage and changes in the expression of metabolic genes) were discussed in this review.

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Both silver ions and silver nanoparticles facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes

Cited by 239 publications

References 70 publications

Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

Bactericidal Antibacterial Mechanism of Plant Synthesized Silver, Gold and Bimetallic Nanoparticles

Antimicrobial metal-based nanoparticles: a review on their synthesis, types and antimicrobial action

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